CAJ | 학술논문

目的观察视网膜中央静脉阻塞(CRVO)黄斑水肿患者房水中血管内皮生长因子(VEGF)、白细胞介素-6(IL-6)和巨噬细胞趋化蛋白-1(MCP-1)的表达水平.方法未经治疗的CRVO继发黄斑水肿患者连续病例40例40只眼纳入研究.其中,男性25例,女性15例;年龄38～76岁.均为初次就诊,既往无治疗史.选择同期单纯白内障行超声乳化白内障吸除手术患者20例20只眼作为对照组.其中,男性10例10只眼,女性10例10只眼.酶联免疫吸附试验测定两组患者房水中VEGF16.、VEGF165b、IL-6和MCP-1的含量;相关性分析VEGF165与IL-6、MCP-1之间的相关性.结果 CRVO组患者房水中VEGF165、IL-6、MCP-1含量中位数分别为1 089.0、165.6、1 253.0 pg/ml;对照组患者各相应检测指标含量中位数分别为168.2、4.7、216.4 pg/ml.CRVO组患者房水中VEGF165、IL-6、MCP-1含量较对照组明显升高,差异均有统计学意义(Z=-4.549、-6.008、-5.343,P＜0.001).CRVO组、对照组患者房水中VEGF165b含量分别为834.0、915.9 pg/ml,差异无统计学意义(Z=-0.207,P＞0.05).CRVO组患者VEGF165 b/VEGF165比值为2.71,对照组患者相应比值为7.28,二者比较,差异有统计学意义(t=-3.007,P＜0.05).相关性分析结果显示,CRVO组患者IL-6与VEGF165呈高度正相关(r=0.526,P=0.001);对照组患者IL-6与VEGF165亦呈正相关趋势(r=0.425,P=0.070).两组患者中MCP-1与VEGF165均未见相关趋势(CRVO组:r=0.211,P＞0.05;对照组:r=-0.019,P＞0.05).结论 CRVO继发黄斑水肿患者房水中VEGF165、IL-6和MCP-1表达量增高,VEGF165b表达量无明显变化,VEGF165b/VEGF165比值降低.
목적 관찰시망막중앙정맥조새(CRVO)황반수종환자방수중혈관내피생장인자(VEGF)、백세포개소-6(IL-6)화거서세포추화단백-1(MCP-1)적표체수평.방법 미경치료적CRVO계발황반수종환자련속병례40례40지안납입연구.기중,남성25례,녀성15례;년령38～76세.균위초차취진,기왕무치료사.선택동기단순백내장행초성유화백내장흡제수술환자20례20지안작위대조조.기중,남성10례10지안,녀성10례10지안.매련면역흡부시험측정량조환자방수중VEGF16.、VEGF165b、IL-6화MCP-1적함량;상관성분석VEGF165여IL-6、MCP-1지간적상관성.결과 CRVO조환자방수중VEGF165、IL-6、MCP-1함량중위수분별위1 089.0、165.6、1 253.0 pg/ml;대조조환자각상응검측지표함량중위수분별위168.2、4.7、216.4 pg/ml.CRVO조환자방수중VEGF165、IL-6、MCP-1함량교대조조명현승고,차이균유통계학의의(Z=-4.549、-6.008、-5.343,P＜0.001).CRVO조、대조조환자방수중VEGF165b함량분별위834.0、915.9 pg/ml,차이무통계학의의(Z=-0.207,P＞0.05).CRVO조환자VEGF165 b/VEGF165비치위2.71,대조조환자상응비치위7.28,이자비교,차이유통계학의의(t=-3.007,P＜0.05).상관성분석결과현시,CRVO조환자IL-6여VEGF165정고도정상관(r=0.526,P=0.001);대조조환자IL-6여VEGF165역정정상관추세(r=0.425,P=0.070).량조환자중MCP-1여VEGF165균미견상관추세(CRVO조:r=0.211,P＞0.05;대조조:r=-0.019,P＞0.05).결론 CRVO계발황반수종환자방수중VEGF165、IL-6화MCP-1표체량증고,VEGF165b표체량무명현변화,VEGF165b/VEGF165비치강저.
Objective To observe the levels of vascular endothelial growth factor (VEGF),interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in aqueous humor of patients with macular edema secondary to central retinal vein occlusion (CRVO).Methods Forty eyes of 40 consecutive patients with macular edema secondary to CRVO (CRVO group) were enrolled in this study.The patients included 25 males and 15 females.The patient age ranged from 38 to 76 years.The control group was 20 patients with senile cataract who underwent phacoemulsification,including 10 males and 10 females.The levels of VEGF165,VEGF165 b,IL-6 and MCP-1 in aqueous humor were determined by enzyme-linked immunosorbent assay.The correlation of VEGF,and IL-6,and MCP-1 were analyzed.Results The median aqueous level of VEGF165,IL-6 and MCP-1 were 1089.0,165.6,1253.0 pg/ml respectively in CRVO group,which were higher than the control group's results (168.2,4.7,216.4 pg/ml respectively),the differences were statistically significant (Z=-4.549,-6.008,-5.343; P＜0.001).The VEGF165b in CRVO group and control group were 834.0,915.9 pg/ml respectively,the difference was not statistically significant (Z=-0.207,P＞0.05).The ratio of VEGF165 b to VEGF165 in CRVO group and control group were 2.71,7.28 respectively,the difference was statistically significant (t=-3.007,P＜0.05).There was a highly positive correlation between IL-6 and VEGF in CRVO group (r=0.526,P=0.001) and also mild positive correlation in control group (r=0.425,P=0.070).No correlation between MCP-1 and VEGF was observed in both groups (CRVO group:r=0.211,P＞0.05.Control group:r=-0.019,P＞0.05).Conclusions VEGF165,IL-6 and MCP-1 levels were increased in CRVO patients while the VEGF165 b was normal.The ratio between VEGF165 b and VEGF165 in aqueous humor of patients with macular edema secondary to CRVO was decreased.