中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2014年
5期
484-487
,共4页
王友%邓铂林%黄健%安刚
王友%鄧鉑林%黃健%安剛
왕우%산박림%황건%안강
糖尿病视网膜病变/治疗%血管生成抑制剂/治疗应用%抗体,单克隆/治疗应用%血管内皮生长因子类%炎症趋化因子类
糖尿病視網膜病變/治療%血管生成抑製劑/治療應用%抗體,單剋隆/治療應用%血管內皮生長因子類%炎癥趨化因子類
당뇨병시망막병변/치료%혈관생성억제제/치료응용%항체,단극륭/치료응용%혈관내피생장인자류%염증추화인자류
Diabetic retinopathy/therapy%Angiogenesis inhibitors/therapeutic use%Antibodies,monoclonal/therapeutic use%Vascular endothelial growth factors%Chemokines
目的 观察严重增生型糖尿病视网膜病变(PDR)患者玻璃体腔注射雷珠单抗后玻璃体细胞因子的变化.方法 临床检查确诊的严重PDR患者80例80只眼纳入研究.依照非主动随机方法将患眼分为单纯玻璃体切割手术组(A组)、玻璃体腔注射雷珠单抗联合玻璃体切割手术组(B组),均为40只眼.两组间性别(x2 =0.05)、年龄(t=0.59)、糖尿病病程(t=0.36)、HbA1c(t=0.13)比较,差异无统计学意义(P＞0.05).A组平均眼压,B组注药前、玻璃体切割手术前平均眼压比较,差异无统计学意义(F=0.81,P＞0.05).A组患眼行玻璃体切割手术时抽取玻璃体液0.4ml.B组患眼玻璃体腔注射雷珠单抗0.05 ml(含雷珠单抗0.5 mg);注药后7d行玻璃体切割手术,抽取玻璃体液0.4 ml.双抗体酶联免疫吸附试验(ELISA)法测定玻璃体血管内皮生长因子(VEGF)、白介素(IL)-6、IL-8、细胞间黏附分子-1(ICAM-1)、结缔组织生长因子(CTGF)浓度.结果 玻璃体VEGF、ICAM-1浓度B组分别为(10.70±3.60)、(224.64±90.32) pg/L,A组分别为(72.38±23.59)、(665.61±203.34) pg/L.两组玻璃体VEGF、ICAM-1浓度比较,差异有统计学意义(t=16.34、12.53,P＜0.001);玻璃体IL-6、IL-8浓度B组分为(210.64±80.27)、(156.00±57.74) pg/L,A组分别为(45.78±33.82)、(41.07±13.82)pg/L.两组玻璃体IL-6、IL-8浓度比较,差异有统计学意义(t=11.97、12.24,P＜0.001).A、B组玻璃体CTGF浓度比较,差异无统计学意义(t=1.39,P＞0.05).B组CTGF/VEGF较A组升高,差异有统计学意义(t=14.75,P＜0.001).结论玻璃体腔注射雷珠单抗1周后VEGF、ICAM-1明显下降;IL-6、IL-8明显升高;CTGF浓度无明显变化,但CTGF/VEGF明显升高.
目的 觀察嚴重增生型糖尿病視網膜病變(PDR)患者玻璃體腔註射雷珠單抗後玻璃體細胞因子的變化.方法 臨床檢查確診的嚴重PDR患者80例80隻眼納入研究.依照非主動隨機方法將患眼分為單純玻璃體切割手術組(A組)、玻璃體腔註射雷珠單抗聯閤玻璃體切割手術組(B組),均為40隻眼.兩組間性彆(x2 =0.05)、年齡(t=0.59)、糖尿病病程(t=0.36)、HbA1c(t=0.13)比較,差異無統計學意義(P＞0.05).A組平均眼壓,B組註藥前、玻璃體切割手術前平均眼壓比較,差異無統計學意義(F=0.81,P＞0.05).A組患眼行玻璃體切割手術時抽取玻璃體液0.4ml.B組患眼玻璃體腔註射雷珠單抗0.05 ml(含雷珠單抗0.5 mg);註藥後7d行玻璃體切割手術,抽取玻璃體液0.4 ml.雙抗體酶聯免疫吸附試驗(ELISA)法測定玻璃體血管內皮生長因子(VEGF)、白介素(IL)-6、IL-8、細胞間黏附分子-1(ICAM-1)、結締組織生長因子(CTGF)濃度.結果 玻璃體VEGF、ICAM-1濃度B組分彆為(10.70±3.60)、(224.64±90.32) pg/L,A組分彆為(72.38±23.59)、(665.61±203.34) pg/L.兩組玻璃體VEGF、ICAM-1濃度比較,差異有統計學意義(t=16.34、12.53,P＜0.001);玻璃體IL-6、IL-8濃度B組分為(210.64±80.27)、(156.00±57.74) pg/L,A組分彆為(45.78±33.82)、(41.07±13.82)pg/L.兩組玻璃體IL-6、IL-8濃度比較,差異有統計學意義(t=11.97、12.24,P＜0.001).A、B組玻璃體CTGF濃度比較,差異無統計學意義(t=1.39,P＞0.05).B組CTGF/VEGF較A組升高,差異有統計學意義(t=14.75,P＜0.001).結論玻璃體腔註射雷珠單抗1週後VEGF、ICAM-1明顯下降;IL-6、IL-8明顯升高;CTGF濃度無明顯變化,但CTGF/VEGF明顯升高.
목적 관찰엄중증생형당뇨병시망막병변(PDR)환자파리체강주사뢰주단항후파리체세포인자적변화.방법 림상검사학진적엄중PDR환자80례80지안납입연구.의조비주동수궤방법장환안분위단순파리체절할수술조(A조)、파리체강주사뢰주단항연합파리체절할수술조(B조),균위40지안.량조간성별(x2 =0.05)、년령(t=0.59)、당뇨병병정(t=0.36)、HbA1c(t=0.13)비교,차이무통계학의의(P＞0.05).A조평균안압,B조주약전、파리체절할수술전평균안압비교,차이무통계학의의(F=0.81,P＞0.05).A조환안행파리체절할수술시추취파리체액0.4ml.B조환안파리체강주사뢰주단항0.05 ml(함뢰주단항0.5 mg);주약후7d행파리체절할수술,추취파리체액0.4 ml.쌍항체매련면역흡부시험(ELISA)법측정파리체혈관내피생장인자(VEGF)、백개소(IL)-6、IL-8、세포간점부분자-1(ICAM-1)、결체조직생장인자(CTGF)농도.결과 파리체VEGF、ICAM-1농도B조분별위(10.70±3.60)、(224.64±90.32) pg/L,A조분별위(72.38±23.59)、(665.61±203.34) pg/L.량조파리체VEGF、ICAM-1농도비교,차이유통계학의의(t=16.34、12.53,P＜0.001);파리체IL-6、IL-8농도B조분위(210.64±80.27)、(156.00±57.74) pg/L,A조분별위(45.78±33.82)、(41.07±13.82)pg/L.량조파리체IL-6、IL-8농도비교,차이유통계학의의(t=11.97、12.24,P＜0.001).A、B조파리체CTGF농도비교,차이무통계학의의(t=1.39,P＞0.05).B조CTGF/VEGF교A조승고,차이유통계학의의(t=14.75,P＜0.001).결론파리체강주사뢰주단항1주후VEGF、ICAM-1명현하강;IL-6、IL-8명현승고;CTGF농도무명현변화,단CTGF/VEGF명현승고.
Objective To observe the concentration of the inflammatory cytokines in vitreous of severe proliferative diabetic retinopathy (PDR) after intravitreal ranibizumab injection (IVR).Methods A total of 80 PDR patients (80 eyes) were enrolled in this study.The patients were randomly divided into vitrectomy group (group A) and IVR combined with vitrectomy group (group B),40 eyes in each group.The differences of sex(x2=0.05),age (t=0.59),duration of diabetes (t=0.36),HbA1c (t=0.13) and intraocular pressure (F=0.81) between two groups were not significant (P＞0.05).The eyes in group B received 0.5 mg (0.05 ml) ranibizumab injection at 7 days before operation.The vitreous samples (0.4 ml)were obtained before operation.The concentration of vascular endothelial growth factor (VEGF),interleukin (IL)-6,IL-8,intercellular adhesion molecule-1 (ICAM-1) and connective tissue growth factor (CTGF) were measured by enzyme-linked immunosorbent assays.Results The concentration of VEGF and ICAM-1 were (10.70±3.60),(224.64±90.32) pg/L in group B and (72.38±23.59),(665.61±203.34)pg/L in group A.The differences of VEGF and ICAM-1 concentration between two groups was significant (t=16.34,12.53 ; P＜ 0.001).The concentration of IL-6 and IL-8 were (210.64 ± 80.27),(156.00±57.74) pg/L in group B and (45.78±33.82),(41.07±13.82) pg/L in group A.The differences of IL-6 and IL-8 concentration between two groups was significant (t=11.97,12.24; P＜0.001).There was no difference of CTGF concentration between two groups (t=1.39,P=0.17).The CTGF/VEGF in group B was higher than that in group A (t=14.75,P＜0.001).Conclusions One week after IVR,the concentration of VEGF and ICAM-1 are decreased,while IL-6 and IL-8 increased.There is no obvious change in CTGF,but CTGF/VEGF is increased.
