中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2011年
12期
1090-1092
,共3页
马娅%侯殿俊%刘伟%李洁清%周桂珍%乔建维%封丽
馬婭%侯殿俊%劉偉%李潔清%週桂珍%喬建維%封麗
마아%후전준%류위%리길청%주계진%교건유%봉려
受体,抗原,T细胞%辐射,电离%生物剂量计
受體,抗原,T細胞%輻射,電離%生物劑量計
수체,항원,T세포%복사,전리%생물제량계
Receptors,antigen,T-Cell%Radiation,ionizing%Biological dosimeter
目的 建立T细胞受体基因突变频率(T cell receptor mutation frequency,TCR MF)与电离辐射剂量效应关系曲线.方法 招募8名健康成年人,4男4女.采集其外周血,分离淋巴细胞,分装到12孔培养板中,不同剂量(0.00～8.00 Gy)照射后,用植物血凝素蛋白(PHA-P)刺激,加人重组白介素-2(IL-2)培育7d,以流式细胞仪测量TCR MF,拟合剂量-效应曲线.另招募16例不同类型、照射方式及照射剂量的癌症放疗患者,采集外周血,用同样方法分离培养淋巴细胞,测量TCR MF,用拟合的剂量-效应曲线估算肿瘤放疗患者全身等效剂量.采集外周血,观察淋巴细胞染色体双着丝粒和着丝粒环畸变情况,估算受照剂量.结果 TCR MF与电离辐射在0.00～8.00 Gy剂量范围内剂量效应关系良好；大剂量组(2.00 ～8.00 Gy)、小剂量组(0.00～1.00 Gy)及0.00～8.00 Gy剂量组拟合曲线均符合二次多项式模式,拟合方程分别为TCR MF=- 32.8579+ 20.5436D+0.6341D2、TCR MF=1.796+0.017D+ 5.155D2和TCR MF=-0.6229+6.305D+0.6919D2,D为辐射剂量(Gy).用实验建立的曲线估算肿瘤放疗患者的全身受照剂量,与用染色体双着丝粒和着丝粒环畸变率估算的剂量平均相对标准偏差为16.8％.结论 应用TCR基因突变分析技术拟合出的辐射剂量效应曲线,可应用于电离辐射事故受照者近期受照剂量的估算.
目的 建立T細胞受體基因突變頻率(T cell receptor mutation frequency,TCR MF)與電離輻射劑量效應關繫麯線.方法 招募8名健康成年人,4男4女.採集其外週血,分離淋巴細胞,分裝到12孔培養闆中,不同劑量(0.00～8.00 Gy)照射後,用植物血凝素蛋白(PHA-P)刺激,加人重組白介素-2(IL-2)培育7d,以流式細胞儀測量TCR MF,擬閤劑量-效應麯線.另招募16例不同類型、照射方式及照射劑量的癌癥放療患者,採集外週血,用同樣方法分離培養淋巴細胞,測量TCR MF,用擬閤的劑量-效應麯線估算腫瘤放療患者全身等效劑量.採集外週血,觀察淋巴細胞染色體雙著絲粒和著絲粒環畸變情況,估算受照劑量.結果 TCR MF與電離輻射在0.00～8.00 Gy劑量範圍內劑量效應關繫良好；大劑量組(2.00 ～8.00 Gy)、小劑量組(0.00～1.00 Gy)及0.00～8.00 Gy劑量組擬閤麯線均符閤二次多項式模式,擬閤方程分彆為TCR MF=- 32.8579+ 20.5436D+0.6341D2、TCR MF=1.796+0.017D+ 5.155D2和TCR MF=-0.6229+6.305D+0.6919D2,D為輻射劑量(Gy).用實驗建立的麯線估算腫瘤放療患者的全身受照劑量,與用染色體雙著絲粒和著絲粒環畸變率估算的劑量平均相對標準偏差為16.8％.結論 應用TCR基因突變分析技術擬閤齣的輻射劑量效應麯線,可應用于電離輻射事故受照者近期受照劑量的估算.
목적 건립T세포수체기인돌변빈솔(T cell receptor mutation frequency,TCR MF)여전리복사제량효응관계곡선.방법 초모8명건강성년인,4남4녀.채집기외주혈,분리림파세포,분장도12공배양판중,불동제량(0.00～8.00 Gy)조사후,용식물혈응소단백(PHA-P)자격,가인중조백개소-2(IL-2)배육7d,이류식세포의측량TCR MF,의합제량-효응곡선.령초모16례불동류형、조사방식급조사제량적암증방료환자,채집외주혈,용동양방법분리배양림파세포,측량TCR MF,용의합적제량-효응곡선고산종류방료환자전신등효제량.채집외주혈,관찰림파세포염색체쌍착사립화착사립배기변정황,고산수조제량.결과 TCR MF여전리복사재0.00～8.00 Gy제량범위내제량효응관계량호；대제량조(2.00 ～8.00 Gy)、소제량조(0.00～1.00 Gy)급0.00～8.00 Gy제량조의합곡선균부합이차다항식모식,의합방정분별위TCR MF=- 32.8579+ 20.5436D+0.6341D2、TCR MF=1.796+0.017D+ 5.155D2화TCR MF=-0.6229+6.305D+0.6919D2,D위복사제량(Gy).용실험건립적곡선고산종류방료환자적전신수조제량,여용염색체쌍착사립화착사립배기변솔고산적제량평균상대표준편차위16.8％.결론 응용TCR기인돌변분석기술의합출적복사제량효응곡선,가응용우전리복사사고수조자근기수조제량적고산.
Objective To establish the dose-effect curve between TCR MF and ionizing radiation.Methods Peripheral lymphocytes were collected from 8 healthy adults(4 males and 4 females) and cultured in vitro with 12 well culture plates.They were stimulated by PHA-P and IL-2 after exposed to different doses of irradiation (0.00 -8.00 Gy) and cultured for 7 d.The dose-effect curve was established after measuring TCR MF using flow cytometry.Also,using the same method,we separated and cultured the peripheral lymphocytes collected from 16 radiotherapy cancer patients,whose radiation styles and doses were different,and then measured TCR MF to estimate the whole equivalent dose of radiotherapy patients through the dose-effect curve.Peripheral blood was collected and cultured,chromosome aberration (dicentric and ring) was determined under microscope to estimate irradiation dose.Results The relationship of dose-effect between the TCR MF and ionizing radiation (0.00 -8.00 Gy) was well,the curve of large dose group (2.00 -8.00 Gy),low dose group(0.00 - 1.00 Gy) and 0.00 -8.00 Gy dose group were met with the quadratic polynomial model,the equation was TCR MF =-32.8579 + 20.5436D + 0.6341D2,TCR MF =1.796 + 0.017D + 5.155D2 and TCR MF =- 0.6229 + 6.305D + 0.6919D2,respectively.D was the radiation dose ( Gy ).Using the established curve and the chromosome aberration method to estimate the systemic exposure dosage,the average relative deviation was 16.8％.Conclusion The curve established by the TCR gene mutation analysis technology can be applied to exposure dose eatimation of victims in ionization radiation accidents.
