中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2012年
11期
987-991
,共5页
潘晓红%张佳峰%丁晓贝%徐云%陈琳%杨介者
潘曉紅%張佳峰%丁曉貝%徐雲%陳琳%楊介者
반효홍%장가봉%정효패%서운%진림%양개자
HIV%获得性免疫缺陷综合征%流行病学,分子%CRF01_AE%基因
HIV%穫得性免疫缺陷綜閤徵%流行病學,分子%CRF01_AE%基因
HIV%획득성면역결함종합정%류행병학,분자%CRF01_AE%기인
HIV%Acquired immunodeficiency syndrome%Epidemiology,molecular%CRF01_AE%Genes
目的 了解2009年浙江省HIV-1型CRF01_AE重组毒株在浙江省随访HIV感染者和艾滋病患者中的分布特征.方法 以浙江省地市为单位进行分层,对2009年随访的HIV感染者和艾滋病患者随机抽样确定303例研究对象,收集调查对象信息并采集样本提取DNA,用PCR方法扩增gag部分基因片段,测定序列并进行分子流行病学分析.结果 303例研究对象中扩增阳性并成功测序的共有225例.其中,CRF01 _AE重组型占58.67％ (132/225).感染CRF01_AE毒株的调查对象中,性传播者占90.91％ (120/132),其中异性性传播占65.91％( 87/132),男男性行为传播者占25.00％ (33/132).系统树分析表明,感染CRF01_AE毒株的HIV感染者及艾滋病患者的病毒系统进化树存在3个分支簇,组内基因遗传距离分别为0.037±0.011、0.034±0.008、0.047 ±0.010(P＜0.05).同性传播感染者序列高度集中在簇1(96.97％,32/33),且与异性传播感染者序列并存,且与江苏、河南郑州、辽宁和河北石家庄流行株有亲缘关系.结论 CRF01_AE毒株在浙江省随访的HIV感染者及艾滋病患者中分布最广,以性传播为主(异性传播居多),同性传播毒株自成体系,但有向异性传播感染者扩散的现象.
目的 瞭解2009年浙江省HIV-1型CRF01_AE重組毒株在浙江省隨訪HIV感染者和艾滋病患者中的分佈特徵.方法 以浙江省地市為單位進行分層,對2009年隨訪的HIV感染者和艾滋病患者隨機抽樣確定303例研究對象,收集調查對象信息併採集樣本提取DNA,用PCR方法擴增gag部分基因片段,測定序列併進行分子流行病學分析.結果 303例研究對象中擴增暘性併成功測序的共有225例.其中,CRF01 _AE重組型佔58.67％ (132/225).感染CRF01_AE毒株的調查對象中,性傳播者佔90.91％ (120/132),其中異性性傳播佔65.91％( 87/132),男男性行為傳播者佔25.00％ (33/132).繫統樹分析錶明,感染CRF01_AE毒株的HIV感染者及艾滋病患者的病毒繫統進化樹存在3箇分支簇,組內基因遺傳距離分彆為0.037±0.011、0.034±0.008、0.047 ±0.010(P＜0.05).同性傳播感染者序列高度集中在簇1(96.97％,32/33),且與異性傳播感染者序列併存,且與江囌、河南鄭州、遼寧和河北石傢莊流行株有親緣關繫.結論 CRF01_AE毒株在浙江省隨訪的HIV感染者及艾滋病患者中分佈最廣,以性傳播為主(異性傳播居多),同性傳播毒株自成體繫,但有嚮異性傳播感染者擴散的現象.
목적 료해2009년절강성HIV-1형CRF01_AE중조독주재절강성수방HIV감염자화애자병환자중적분포특정.방법 이절강성지시위단위진행분층,대2009년수방적HIV감염자화애자병환자수궤추양학정303례연구대상,수집조사대상신식병채집양본제취DNA,용PCR방법확증gag부분기인편단,측정서렬병진행분자류행병학분석.결과 303례연구대상중확증양성병성공측서적공유225례.기중,CRF01 _AE중조형점58.67％ (132/225).감염CRF01_AE독주적조사대상중,성전파자점90.91％ (120/132),기중이성성전파점65.91％( 87/132),남남성행위전파자점25.00％ (33/132).계통수분석표명,감염CRF01_AE독주적HIV감염자급애자병환자적병독계통진화수존재3개분지족,조내기인유전거리분별위0.037±0.011、0.034±0.008、0.047 ±0.010(P＜0.05).동성전파감염자서렬고도집중재족1(96.97％,32/33),차여이성전파감염자서렬병존,차여강소、하남정주、료녕화하북석가장류행주유친연관계.결론 CRF01_AE독주재절강성수방적HIV감염자급애자병환자중분포최엄,이성전파위주(이성전파거다),동성전파독주자성체계,단유향이성전파감염자확산적현상.
Objective To analyze the molecular epidemiological characteristics of HIV-1 subtype CRF01_AE strains being prevailed among HIV/AIDS in Zhejiang province in 2009.Methods A total of 303 subjects were identified by stratified random sampling among HIV infected individuals in Zhejiang province in 2009.Gag fragments of the HIV-1 strains were amplified by nested polymerase chain reaction from the DNA extracted from whole blood of HIV-1 infected individuals.PCR products were sequenced and analyzed by phylogenetic method.Results A total of 132 HIV-1 subtype CRF01_AE sequences were identified from the 225 samples that sequenced successfully,accounting for 58.67％ ( 132/225 ).A total of 90.91％ (120/132) CRF01_AE strains infected HIV/AIDS were transmitted mainly by sexual contacts.A total of 65.91％ (87/132) of the cases infected by heterosexual route and 25.00％ (33/132) by homosexual route.There were three main clusters in the phylogenetic tree.Pairwise DNA distance within three groups was 0.037 ± 0.011,0.034 ± 0.008 and 0.047 ± 0.010,which has statistical significance ( P ＜ 0.05 ).Distribution of the sequence of homosexual behavior infected individuals was relatively concentrated in clusters one(96.97％,32/33 ),and crossed with heterosexual behavior infectors,and presented the close relations with strains from Jiangsu province, Zhengzhou of Henan province, Liaoning province, Shijiazhuang of Hebei province.Conclusion The CRF01 _AE strains were the dominant subtypes among HIV infected individuals.The majority of the CRF01 _AE infected cases had high risk sexual behavior.The heterosexual infected cases were more than homosexual cases.The circulating status of CRF01 _AE strains in homosexual population was relatively independent, but also had evidence of transmission from man who have sex with man to heterosexual population.
