CAJ | 학술논문

目的对山东省73株生物1A型小肠结肠炎耶尔森菌分离株进行PFGE分型,分析菌株相关性及PFGE分型与菌株生物学特征的联系.方法于2008-2009年在山东省高密市和五莲县2个监测点的动物粪便和肉制品中共分离生物1A型小肠结肠炎耶尔森菌73株.通过动力试验、血清凝集实验和毒力基因检测了解菌株生物学特征；采用PFGE对菌株进行分子分型,并分析PFGE分型与动力、血清型和毒力基因型的关系.结果 73株菌中5株动力中等,其余68株动力良好.血清型O∶5(17/73)和O∶8(14/73)为优势血清型,其次为O∶9(5/73)、O∶7,8(1/73),未见O∶3血清型,另有36株菌血清型无法确定.黏附侵袭位点基因(ail)、耐热肠毒素A基因(ystA)、黏附素基因(yadA)和毒力调节因子基因(virF)等毒力基因检测均为阴性,耐热肠毒素B基因(ystB)阳性率为72.6％ (53/73).73株菌可分为54个PFGE型别(K6GN11SD0001 ～K6GN11SD0054),大部分型别只含1～2株菌,无优势PFGE型.相同或相近的聚类簇中,均包含不同宿主来源的菌株；各血清型,毒力基因型在聚类树中分散存在,与PFGE分型亦无特异性关联.5株动力中等的菌株有4株聚类在同一分支,相似度为80.9％～ 100.0％,且毒力基因型均为B型.结论生物1A型小肠结肠炎耶尔森菌存在多克隆系,PFGE分型与血清型、毒力基因型、宿主未发现有联系,与动力有定相关性.
목적 대산동성73주생물1A형소장결장염야이삼균분리주진행PFGE분형,분석균주상관성급PFGE분형여균주생물학특정적련계.방법 우2008-2009년재산동성고밀시화오련현2개감측점적동물분편화육제품중공분리생물1A형소장결장염야이삼균73주.통과동력시험、혈청응집실험화독력기인검측료해균주생물학특정；채용PFGE대균주진행분자분형,병분석PFGE분형여동력、혈청형화독력기인형적관계.결과 73주균중5주동력중등,기여68주동력량호.혈청형O∶5(17/73)화O∶8(14/73)위우세혈청형,기차위O∶9(5/73)、O∶7,8(1/73),미견O∶3혈청형,령유36주균혈청형무법학정.점부침습위점기인(ail)、내열장독소A기인(ystA)、점부소기인(yadA)화독력조절인자기인(virF)등독력기인검측균위음성,내열장독소B기인(ystB)양성솔위72.6％ (53/73).73주균가분위54개PFGE형별(K6GN11SD0001 ～K6GN11SD0054),대부분형별지함1～2주균,무우세PFGE형.상동혹상근적취류족중,균포함불동숙주래원적균주；각혈청형,독력기인형재취류수중분산존재,여PFGE분형역무특이성관련.5주동력중등적균주유4주취류재동일분지,상사도위80.9％～ 100.0％,차독력기인형균위B형.결론 생물1A형소장결장염야이삼균존재다극륭계,PFGE분형여혈청형、독력기인형、숙주미발현유련계,여동력유정상관성.
Objective To investigate the molecular subtypes of 73 strains of Yersinia enterocolitica biotype 1A isolated in Shandong province by PFGE,and thereby to analyze the relationship between PFGE typing and biological characteristics.Methods Seventy-three strains of Yersinia enterocolitica biotype 1A were isolated from animal feces and meat pnoducts in Gaomi city and Wulian county in Shandong province from 2008 to 2009.Motility test,serum agglutination and virulent genes detection by PCR were used to learn the biological characteristics of the isolated strains.The molecular subtypes were determined by PFGE,whose relationships with motility,serotypes and virulent genotypes were also analyzed.Results Out of the 73strains of Yersinia enterocolitica,5 showed medium-active motility while the other 68 showed well-active motility.The dominated serotypes were O∶ 5 (17/73) and O∶ 8 (14/73),followed by O∶ 9 (5/73) and O∶ 7,8(1/73),and there was no O∶ 3 serotype found.Meanwhile,36 strains couldn't be serotyped.All the strains were negative with the gene ail,ystA,yadA and virF,yet the positive rate of ystB gene was 72.6％(53/73).The 73 strains of Yersinia enterocolitica isolated could be subtyped into 54 PFGE patterns (K6GN11SD0001-K6GN11SD0054),most of which only had 1 or 2 isolated strains,and no pattern was dominant.The strains in the same or similar cluster were from different hosts; each serotype and toxic genotype scattered in the clustering trees,without specific correlation with PFGE subtypes.4 out of 5 strains,which showed medium-active motility,belonged to one branch,with the similarity coefficient at 80.9％-100.0％ ; while all the toxic genotype belonged to type B.Conclusion Biotype 1A Yersinia enterocolitica has many clones,whose PFGE types had relations with motility but no relations with virulent genotype and host.