CAJ | 학술논문

目的调查2009-2011年广州诺如病毒的分子流行病学特征.方法分别收集广州市2010年1月至2011年5月的环境水样183份、2009年6月至2011年6月的海产品1162份和2009年7月至2010年12月的腹泻患者粪便标本1066份,以实时荧光定量逆转录PCR (qRT-PCR)检测诺如病毒,对扩增阳性标本测序后与GenBank中的诺如病毒参考株比较,绘制系统进化树.结果环境水样中诺如病毒阳性率为19.67％(36/183),海产品中的阳性率为8.26％(96/1162),腹泻患者粪便标本中的阳性率为37.05％(395/1066);扩增阳性样品中的诺如病毒可分为10株代表株,其中7个代表株(共208份标本)的基因型为G2-4型,并且水样、海产品和粪便标本同源性很高,相似度达94％～ 100％.结论广州诺如病毒流行优势株为G2-4型,同时标本中阳性率较高.
목적 조사2009-2011년엄주낙여병독적분자류행병학특정.방법 분별수집엄주시2010년1월지2011년5월적배경수양183빈、2009년6월지2011년6월적해산품1162빈화2009년7월지2010년12월적복사환자분편표본1066빈,이실시형광정량역전록PCR (qRT-PCR)검측낙여병독,대확증양성표본측서후여GenBank중적낙여병독삼고주비교,회제계통진화수.결과배경수양중낙여병독양성솔위19.67％(36/183),해산품중적양성솔위8.26％(96/1162),복사환자분편표본중적양성솔위37.05％(395/1066);확증양성양품중적낙여병독가분위10주대표주,기중7개대표주(공208빈표본)적기인형위G2-4형,병차수양、해산품화분편표본동원성흔고,상사도체94％～ 100％.결론 엄주낙여병독류행우세주위G2-4형,동시표본중양성솔교고.
Objective To investigate the molecular epidemiological characteristics of norovirus in Guangzhou from 2009 to 2011.Methods A total of 183 water samples,1162 seafood samples and 1066 diarrhea stool specimens were collected from January 2010 to May 2011,June 2009 to June 2011 and July 2009 to December 2010 respectively in Guangzhou.Norovirus was detected by real time reverse transcript-PCR(qRT-PCR).The partial polymerase gene was amplified from norovirus positive samples,then sequenced and compared with the sequences of norovirus in GenBank.The phylogenetic tree was created.Results The positive rate was 19.67％ (36/183),8.26％ (96/1162) and 37.05％ (395/1066)in water samples,seafood and diarrhea patients respectively.Noroviruscs from positive samples could be divided into 10 representative strains,in which 7 representative strains of genotype of 208 samples was type G2-4.The sequences from water,seafood and stool specimens were highly homologous with the similarity of 94％-100％.Conclusion In Guangzhou,the predominant Norovirus genotype was G24 and the positive rate of samples was high.