中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2013年
1期
55-58
,共4页
田明杰%宋江南%刘培培%苏立宏%孙长颢%李颖
田明傑%宋江南%劉培培%囌立宏%孫長顥%李穎
전명걸%송강남%류배배%소립굉%손장호%리영
小鼠%血糖%β葡聚糖类%血脂
小鼠%血糖%β葡聚糖類%血脂
소서%혈당%β포취당류%혈지
Mice%Blood glucose%Beta-Glucans%Blood lipid
目的 研究青稞中β-葡聚糖对高脂膳食诱导的C57小鼠血糖、血脂的影响.方法 取C57B L/6雄性小鼠40只,采用随机数字表法,按体重分层随机分为4组(每组10只):β-葡聚糖高剂量组(4%β-葡聚糖+高脂饲料)、低剂量组(2%β-葡聚糖+高脂饲料)、高脂组及基础组.观察其摄食量及体重变化情况.干预的第11周末,进行葡萄糖耐量试验,并测定空腹血糖.第12周末测其体重,处死小鼠,留取血液标本测定血液相关生化指标.结果 β-葡聚糖高剂量组、β-葡聚糖低剂量组、高脂组和基础组体重分别为(32.8±1.5)、(40.4±1.9)、(40.7±2.1)、(33.5±1.3)g(F =55.26,P<0.05);平均摄食量分别为(3.48±0.56)、(3.69±0.76)、(3.66 ±0.81)、(3.54±0.61)g/d(F=0.26,P>0.05);空腹血糖分别为(5.29±1.59)、(6.13±1.75)、(7.63±1.09)、(4.24 ±0.98) mmol/L (F =9.54,P<0.01);血清胰岛素水平分别为(1.97 ±0.10)、(2.44 ±0.24)、(3.02 ±0.36)、(1.48±0.28) ng/ml(F=47.58,P<0.01);葡萄糖耐量试验曲线下面积分别为(25.81 ±1.44)、(30.42 ±2.01)、(35.17 ±1.20)、(21.03±1.24) mmol· L-1· h-1 (F=64.98,P<0.05);胰岛素抵抗指数分别为(9.84±3.78)、(13.69 ±4.48)、(21.54 ±3.27)、(5.81±1.59)(F=30.18,P<0.01);血清TC水平分别为(4.05 ±0.88)、(4.30±0.48)、(4.73 ±0.66)、(3.37±0.40) mmol/L(F=6.70,P<0.01);血清TG水平分别为(0.90±0.09)、(0.98±0.09)、(1.05±0.06)、(0.76±0.26) mmol/L(F=6.75,P<0.01);血清HDL-C水平分别为(2.91±0.59)、(3.34±0.46)、(4.89 ±0.42)、(3.24±0.37)mmol/L (F=31.73,P<0.01);血清LDL-C水平分别为(0.25±0.15)、(0.42 ±0.19)、(0.72 ±0.12)、(0.32 ±0.11) mmol/L(F=17.27,P<0.01);血清游离脂肪酸水平分别为(1.06 ±0.03)、(1.05 ±0.05)、(1.18 ±0.32)、(1.04 ±0.02)mmol/L (F=1.36,P>0.05); HDL-C/LDL-C分别为(13.77 ±5.51)、(9.11 ±3.53)、(7.04±1.65)、(11.21 ±3.31) (F=5.24,P<0.01).结论 青稞β-葡聚糖能够降低高脂诱导的C57小鼠血糖和血脂,并且可降低胰岛素抵抗和动脉硬化的风险.
目的 研究青稞中β-葡聚糖對高脂膳食誘導的C57小鼠血糖、血脂的影響.方法 取C57B L/6雄性小鼠40隻,採用隨機數字錶法,按體重分層隨機分為4組(每組10隻):β-葡聚糖高劑量組(4%β-葡聚糖+高脂飼料)、低劑量組(2%β-葡聚糖+高脂飼料)、高脂組及基礎組.觀察其攝食量及體重變化情況.榦預的第11週末,進行葡萄糖耐量試驗,併測定空腹血糖.第12週末測其體重,處死小鼠,留取血液標本測定血液相關生化指標.結果 β-葡聚糖高劑量組、β-葡聚糖低劑量組、高脂組和基礎組體重分彆為(32.8±1.5)、(40.4±1.9)、(40.7±2.1)、(33.5±1.3)g(F =55.26,P<0.05);平均攝食量分彆為(3.48±0.56)、(3.69±0.76)、(3.66 ±0.81)、(3.54±0.61)g/d(F=0.26,P>0.05);空腹血糖分彆為(5.29±1.59)、(6.13±1.75)、(7.63±1.09)、(4.24 ±0.98) mmol/L (F =9.54,P<0.01);血清胰島素水平分彆為(1.97 ±0.10)、(2.44 ±0.24)、(3.02 ±0.36)、(1.48±0.28) ng/ml(F=47.58,P<0.01);葡萄糖耐量試驗麯線下麵積分彆為(25.81 ±1.44)、(30.42 ±2.01)、(35.17 ±1.20)、(21.03±1.24) mmol· L-1· h-1 (F=64.98,P<0.05);胰島素牴抗指數分彆為(9.84±3.78)、(13.69 ±4.48)、(21.54 ±3.27)、(5.81±1.59)(F=30.18,P<0.01);血清TC水平分彆為(4.05 ±0.88)、(4.30±0.48)、(4.73 ±0.66)、(3.37±0.40) mmol/L(F=6.70,P<0.01);血清TG水平分彆為(0.90±0.09)、(0.98±0.09)、(1.05±0.06)、(0.76±0.26) mmol/L(F=6.75,P<0.01);血清HDL-C水平分彆為(2.91±0.59)、(3.34±0.46)、(4.89 ±0.42)、(3.24±0.37)mmol/L (F=31.73,P<0.01);血清LDL-C水平分彆為(0.25±0.15)、(0.42 ±0.19)、(0.72 ±0.12)、(0.32 ±0.11) mmol/L(F=17.27,P<0.01);血清遊離脂肪痠水平分彆為(1.06 ±0.03)、(1.05 ±0.05)、(1.18 ±0.32)、(1.04 ±0.02)mmol/L (F=1.36,P>0.05); HDL-C/LDL-C分彆為(13.77 ±5.51)、(9.11 ±3.53)、(7.04±1.65)、(11.21 ±3.31) (F=5.24,P<0.01).結論 青稞β-葡聚糖能夠降低高脂誘導的C57小鼠血糖和血脂,併且可降低胰島素牴抗和動脈硬化的風險.
목적 연구청과중β-포취당대고지선식유도적C57소서혈당、혈지적영향.방법 취C57B L/6웅성소서40지,채용수궤수자표법,안체중분층수궤분위4조(매조10지):β-포취당고제량조(4%β-포취당+고지사료)、저제량조(2%β-포취당+고지사료)、고지조급기출조.관찰기섭식량급체중변화정황.간예적제11주말,진행포도당내량시험,병측정공복혈당.제12주말측기체중,처사소서,류취혈액표본측정혈액상관생화지표.결과 β-포취당고제량조、β-포취당저제량조、고지조화기출조체중분별위(32.8±1.5)、(40.4±1.9)、(40.7±2.1)、(33.5±1.3)g(F =55.26,P<0.05);평균섭식량분별위(3.48±0.56)、(3.69±0.76)、(3.66 ±0.81)、(3.54±0.61)g/d(F=0.26,P>0.05);공복혈당분별위(5.29±1.59)、(6.13±1.75)、(7.63±1.09)、(4.24 ±0.98) mmol/L (F =9.54,P<0.01);혈청이도소수평분별위(1.97 ±0.10)、(2.44 ±0.24)、(3.02 ±0.36)、(1.48±0.28) ng/ml(F=47.58,P<0.01);포도당내량시험곡선하면적분별위(25.81 ±1.44)、(30.42 ±2.01)、(35.17 ±1.20)、(21.03±1.24) mmol· L-1· h-1 (F=64.98,P<0.05);이도소저항지수분별위(9.84±3.78)、(13.69 ±4.48)、(21.54 ±3.27)、(5.81±1.59)(F=30.18,P<0.01);혈청TC수평분별위(4.05 ±0.88)、(4.30±0.48)、(4.73 ±0.66)、(3.37±0.40) mmol/L(F=6.70,P<0.01);혈청TG수평분별위(0.90±0.09)、(0.98±0.09)、(1.05±0.06)、(0.76±0.26) mmol/L(F=6.75,P<0.01);혈청HDL-C수평분별위(2.91±0.59)、(3.34±0.46)、(4.89 ±0.42)、(3.24±0.37)mmol/L (F=31.73,P<0.01);혈청LDL-C수평분별위(0.25±0.15)、(0.42 ±0.19)、(0.72 ±0.12)、(0.32 ±0.11) mmol/L(F=17.27,P<0.01);혈청유리지방산수평분별위(1.06 ±0.03)、(1.05 ±0.05)、(1.18 ±0.32)、(1.04 ±0.02)mmol/L (F=1.36,P>0.05); HDL-C/LDL-C분별위(13.77 ±5.51)、(9.11 ±3.53)、(7.04±1.65)、(11.21 ±3.31) (F=5.24,P<0.01).결론 청과β-포취당능구강저고지유도적C57소서혈당화혈지,병차가강저이도소저항화동맥경화적풍험.
Objective Study the effects of β-glucan in highland barley on blood glucose and serum lipid in high fat diet induced C57 mouse.Methods Using table of random number,40 male C57BL/6 mice were randomly divided into 4 groups (10 mice in each group) by weight:high dosage group (4% β-glucan and high fat diet),low dosage group (2% β-glucan and high fat diet),high fat diet group and normal control group.Food-intake and body weight of C57 mouse were observed.Glucose tolerance tests and examinations of fasting blood glucose were performed at the end of 11 weeks of intervention.Mice were sacrificed after 12 wk of treatment,and serum specimens were obtained to test relevant biochemical indicators.Results After 12 weeks raise,among high dosage group,low dosage group,high fat diet group and normal control group,the weight was (32.8 ± 1.5),(40.4 ± 1.9),(40.7 ± 2.1) and (33.5 ± 1.3) g,respectively (F =55.26,P <0.05) ; average food intake was (3.48 ±0.56),(3.69 ±0.76),(3.66 ±0.81) and (3.54 ±0.61) g/d respectively (F =0.26,P > 0.05) ; fasting blood-glucose was (5.29 ± 1.59),(6.13 ± 1.75),(7.63 ±1.09) and (4.24 ±0.98) mmol/L respectively (F =9.54,P < 0.01) ; serum insulin level was (1.97 ±0.10),(2.44 ±0.24),(3.02 ±0.36) and (1.48 ±0.28) ng/ml respectively (F =47.58,P <0.01) ; the area under blood glucose concentration curve was (25.81 ± 1.44),(30.42 ± 2.01),(35.17 ± 1.20) and (21.03 ± 1.24) mmol · L-1 · h 1,respectively (F =64.98,P < 0.05) ; insulin resistance index was (9.84±3.78),(13.69 ±4.48),(21.54 ±3.27) and (5.81 ±1.59) respectively (F=30.18,P <0.01) ; serum total cholesterol (TC) level was (4.05 ± 0.88),(4.30 ± 0.48),(4.73 ± 0.66) and(3.37 ± 0.40) mmol/L respectively (F =6.70,P < 0.01); serum triglyceride (TG) level was(0.90 ± 0.09),(0.98 ± 0.09),(1.05 ± 0.06) and (0.76 ± 0.26) mmol/L respectively (F =6.75,P <0.01) ; serum high-density lipoprotein cholesterol (HDL-C) level was (2.91 ± 0.59),(3.34 ± 0.46),(4.89 ± 0.42) and (3.24 ± 0.37) mmol/L respectively (F =31.73,P <0.01) ; serum low-density lipoprotein cholesterol(LDL-C) level was (0.25 ± 0.15),(0.42 ± 0.19),(0.72 ± 0.12) and (0.32 ±0.11) mmol/L,respectively (F =17.27,P < 0.01) ; free fatty acids (FFA) level was (1.06 ± 0.03),(1.05 ± 0.05),(1.18 ± 0.32) and (1.04 ±0.02) mmol/L,respectively (F =1.36,P >0.05);HDL-C/LDL-C was (13.77 ±5.51),(9.11 ±3.53),(7.04 ± 1.65) and (11.21 ±3.31),respectively (F =5.24,P < 0.01).Conclusion The β-glucan in highland barley reduced the serum glucose and serum lipid,as well as insulin resistance and the risk of arterial sclerosis in high-fat induced C57 mouse.