目的 分析江西省脑膜炎奈瑟菌(Neisseria meningitidis,Nm)分离株的分子分型和菌群结构.方法 选取于1976-1987年和2005-2008年在江西省分离自患者、密切接触者和健康人群的123株Nm菌株作为研究对象.采用多位点序列分型(MLST)、外膜蛋白编码基因proA PCR扩增并测序等方法,检测Nm基因型特征及porA的序列特征.使用BioNumerics软件构建最小生成树,分析菌株菌群结构.结果 1976-1987年分离67株Nm菌,血清群分布为A群(43株)、B群(18株)、C群(1株)和W135群(5株)；2005-2008年分离56株Nm菌,血清群分布为A群(3株)、B群(7株)、C群(45株)和不可分群(1株).123株Nm菌株分为40个序列(ST)型,46株A群菌株分为14个ST型,以ST-3型为优势型别,共29株菌,占A群菌株的63.0％(29/46),分离于1976年至1987年,分离自患者(14株)、密切接触者(9株)、健康人群(6株)；46株C群菌株分为5个ST型,以ST-4821为优势型别,共41株,占89.1％(41/46),分离于2005年至2008年,分离自患者(6株)、密切接触者(6株)、健康人群(29株).123株Nm菌株的porA基因分属于32个不同的型别,包含了24个不同VR1型和22个VR2型.1976-1987年的流行菌株为ST-3型A群Nm,PorA分型为P1.7-1,10,为优势型别,菌株数为18株,占A群Nm的39.1％ (18/46),分离自患者(11株)、密切接触者(4株)、健康人群(3株)；2005-2008年的流行菌株为ST-4821型C群Nm,PorA分型2005年以P1.20,9为主,共19株,占ST-4821型C群Nm流行菌株的46.3％(19/41),分离自密切接触者(1株)、健康人群(18株)；2006年开始以P1.7-2,14型为主,共22株,占ST-4821型C群Nm流行菌株53.7％(22/41),分离自患者(6株)、密切接触者(5株)、健康人群(11株)；B群菌株不存在优势序列群,呈多型性特点；W135群菌株5株均为ST-174序列群,PorA分型为P1.21,16,分离于1979年至1980年,分离自密切接触者(3株)、健康人群(2株).结论 江西省Nm分离株具有基因多态性,同时也存在优势克隆群,在不同时期存在不同的流行克隆；流行克隆已由A:ST-3:P1.7-1,10变迁为C:ST-4821:P1.7-2,14.
目的 分析江西省腦膜炎奈瑟菌(Neisseria meningitidis,Nm)分離株的分子分型和菌群結構.方法 選取于1976-1987年和2005-2008年在江西省分離自患者、密切接觸者和健康人群的123株Nm菌株作為研究對象.採用多位點序列分型(MLST)、外膜蛋白編碼基因proA PCR擴增併測序等方法,檢測Nm基因型特徵及porA的序列特徵.使用BioNumerics軟件構建最小生成樹,分析菌株菌群結構.結果 1976-1987年分離67株Nm菌,血清群分佈為A群(43株)、B群(18株)、C群(1株)和W135群(5株)；2005-2008年分離56株Nm菌,血清群分佈為A群(3株)、B群(7株)、C群(45株)和不可分群(1株).123株Nm菌株分為40箇序列(ST)型,46株A群菌株分為14箇ST型,以ST-3型為優勢型彆,共29株菌,佔A群菌株的63.0％(29/46),分離于1976年至1987年,分離自患者(14株)、密切接觸者(9株)、健康人群(6株)；46株C群菌株分為5箇ST型,以ST-4821為優勢型彆,共41株,佔89.1％(41/46),分離于2005年至2008年,分離自患者(6株)、密切接觸者(6株)、健康人群(29株).123株Nm菌株的porA基因分屬于32箇不同的型彆,包含瞭24箇不同VR1型和22箇VR2型.1976-1987年的流行菌株為ST-3型A群Nm,PorA分型為P1.7-1,10,為優勢型彆,菌株數為18株,佔A群Nm的39.1％ (18/46),分離自患者(11株)、密切接觸者(4株)、健康人群(3株)；2005-2008年的流行菌株為ST-4821型C群Nm,PorA分型2005年以P1.20,9為主,共19株,佔ST-4821型C群Nm流行菌株的46.3％(19/41),分離自密切接觸者(1株)、健康人群(18株)；2006年開始以P1.7-2,14型為主,共22株,佔ST-4821型C群Nm流行菌株53.7％(22/41),分離自患者(6株)、密切接觸者(5株)、健康人群(11株)；B群菌株不存在優勢序列群,呈多型性特點；W135群菌株5株均為ST-174序列群,PorA分型為P1.21,16,分離于1979年至1980年,分離自密切接觸者(3株)、健康人群(2株).結論 江西省Nm分離株具有基因多態性,同時也存在優勢剋隆群,在不同時期存在不同的流行剋隆；流行剋隆已由A:ST-3:P1.7-1,10變遷為C:ST-4821:P1.7-2,14.
목적 분석강서성뇌막염내슬균(Neisseria meningitidis,Nm)분리주적분자분형화균군결구.방법 선취우1976-1987년화2005-2008년재강서성분리자환자、밀절접촉자화건강인군적123주Nm균주작위연구대상.채용다위점서렬분형(MLST)、외막단백편마기인proA PCR확증병측서등방법,검측Nm기인형특정급porA적서렬특정.사용BioNumerics연건구건최소생성수,분석균주균군결구.결과 1976-1987년분리67주Nm균,혈청군분포위A군(43주)、B군(18주)、C군(1주)화W135군(5주)；2005-2008년분리56주Nm균,혈청군분포위A군(3주)、B군(7주)、C군(45주)화불가분군(1주).123주Nm균주분위40개서렬(ST)형,46주A군균주분위14개ST형,이ST-3형위우세형별,공29주균,점A군균주적63.0％(29/46),분리우1976년지1987년,분리자환자(14주)、밀절접촉자(9주)、건강인군(6주)；46주C군균주분위5개ST형,이ST-4821위우세형별,공41주,점89.1％(41/46),분리우2005년지2008년,분리자환자(6주)、밀절접촉자(6주)、건강인군(29주).123주Nm균주적porA기인분속우32개불동적형별,포함료24개불동VR1형화22개VR2형.1976-1987년적류행균주위ST-3형A군Nm,PorA분형위P1.7-1,10,위우세형별,균주수위18주,점A군Nm적39.1％ (18/46),분리자환자(11주)、밀절접촉자(4주)、건강인군(3주)；2005-2008년적류행균주위ST-4821형C군Nm,PorA분형2005년이P1.20,9위주,공19주,점ST-4821형C군Nm류행균주적46.3％(19/41),분리자밀절접촉자(1주)、건강인군(18주)；2006년개시이P1.7-2,14형위주,공22주,점ST-4821형C군Nm류행균주53.7％(22/41),분리자환자(6주)、밀절접촉자(5주)、건강인군(11주)；B군균주불존재우세서렬군,정다형성특점；W135군균주5주균위ST-174서렬군,PorA분형위P1.21,16,분리우1979년지1980년,분리자밀절접촉자(3주)、건강인군(2주).결론 강서성Nm분리주구유기인다태성,동시야존재우세극륭군,재불동시기존재불동적류행극륭；류행극륭이유A:ST-3:P1.7-1,10변천위C:ST-4821:P1.7-2,14.
Objective To study the molecular subtypes and microflora structure of Neisseria meningitidis (Nm) strains isolated in Jiangxi province.Methods A total of 123 Nm strains separately isolated from patients,close contacts and health people in 1976-1987 and 2005-2008 were investigated by multilocus sequence typing (MLST) and PorA subtyping,to test the characteristics of gene Nm and sequence porA.Minimum spanning tree was constructed by using BioNumerics software based on data of MLST; and the microflora structure was then analyzed.Results The serogroups of 67 Nm strains isolated in 1976-1987 included group A (43 strains),group B (18 strains),group C (1 strains) and group W135 (5 strains) ; while the serogroups of 56 Nm strains isolated in 2005-2008 included group A (3 strains),group B (7 strains),group C (45 strains) and 1 ungrouped strain.The total 123 Nm strains could be divided into 40 MLST types;while the 46 strains in group A could be divided into 14 MLST types,29 out of which belonged to ST-3 type,accounting for 63.0％ (29/46) as the dominant type.All of the 29 strains were isolated between 1976 and 1987,while 14 strains were isolated from patients,9 were from close contacts and 6 were from health people.The 46 strains in group C could be divided into 5 MLST types,41 out of which belonged to ST-4821 type,accounting for 89.1％ (41/46).All of the strains were isolated between 2005 and 2008,6 strains were isolated from patients,6 were from close contacts and 29 were from health people.The porA gene of the total 123 Nm strains were classified to 32 different types,including 24 different VR1 types and 22 different VR2 types.The dominant PorA type of the prevalent strain (ST-3 type,group A) between 1976 and 1987 was P1.7-1,10,accounting for 39.1％ (18/46) of the strains in group A; while the 18 strains were isolated from 11 patients,4 close contacts and 3 health people.The dominant PorA type of the prevalent strain (ST-4821 type,group C) between 2005 and 2008 was P1.20,9,accounting for 46.3％ (19/41) of the ST-4821 strains in group C; while the 19 strains were isolated from 1 close contacts and 18 health people.P1.7-2,14 dominated since 2006,including 22 strains,accounting for 53.7％ (22/41) of the ST-4821 strains in group C,isolated from 6 patients,5 close contacts and 11 health people.There were no dominant PorA type found in group B and all the 5 strains in group W135 belonged to ST-174 and the PorA type was P1.21,16,isolating from 3 close contacts and 2 health people between 1979 and 1980.Conclusion Nm isolated in Jiangxi province showed significant gene polymorphism,as well as predominant lineages existing.In different periods,the prevalent lineages varied a lot,as translating from serogroup A:ST-3:P1.7-1,10 to serogroup C:ST-4821:P1.7-2,14 nowadays.
