CAJ | 학술논문

目的采用基于准种分析的HIV暴露后感染溯源检测技术调查一起疑似输血传播HIV案件.方法采集可疑传播链上3例HIV感染者(编号T1～T3)及作为对照的13例已知HIV感染者或艾滋病患者(编号C1～C13)的血样,共计16份血样.提取血浆RNA后,进行逆转录套式PCR扩增,对扩增成功的PCR产物进行克隆、测序.利用BioEdit 6.0.7和MEGA 4.0软件分析基因序列,计算基因离散率、构建系统进化树.结果成功获得了13份样品的核酸序列,T1、T2和T3样品中毒株为CRF07_BC重组亚型,与T2和T3来自同一城市的6份对照样品中有5份毒株为CRF07_BC重组亚型,而与T1来自同一城市的4份对照样品中毒株全部为CRF01_AE重组亚型.T1与T2间的平均基因离散率最小(2.0％),随后依次为C12(2.8％)和T3(2.9％)等.系统进化树分析显示,T1、T2、T3和C12的所有克隆聚为一簇,并提示HIV的传播方向是从T3经T2到T1.结论实验结果支持HIV从T3经T2传播到T1的流行病学调查线索,显示分子流行病学技术可以为HIV暴露后感染溯源调查提供更直接的证据.
목적 채용기우준충분석적HIV폭로후감염소원검측기술조사일기의사수혈전파HIV안건.방법 채집가의전파련상3례HIV감염자(편호T1～T3)급작위대조적13례이지HIV감염자혹애자병환자(편호C1～C13)적혈양,공계16빈혈양.제취혈장RNA후,진행역전록투식PCR확증,대확증성공적PCR산물진행극륭、측서.이용BioEdit 6.0.7화MEGA 4.0연건분석기인서렬,계산기인리산솔、구건계통진화수.결과 성공획득료13빈양품적핵산서렬,T1、T2화T3양품중독주위CRF07_BC중조아형,여T2화T3래자동일성시적6빈대조양품중유5빈독주위CRF07_BC중조아형,이여T1래자동일성시적4빈대조양품중독주전부위CRF01_AE중조아형.T1여T2간적평균기인리산솔최소(2.0％),수후의차위C12(2.8％)화T3(2.9％)등.계통진화수분석현시,T1、T2、T3화C12적소유극륭취위일족,병제시HIV적전파방향시종T3경T2도T1.결론 실험결과지지HIV종T3경T2전파도T1적류행병학조사선색,현시분자류행병학기술가이위HIV폭로후감염소원조사제공경직접적증거.
Objective A molecular technique based on quasispecies analysis for tracing postexposure HIV transmission was applied in an investigation of a possible case of HIV transmission after blood transfusion.Methods Sixteen plasma specimens were collected from 3 HIV infections (T1-T3)involved in a possible HIV transmission chain and 13 HIV/AIDS (C1-C13) controls.The RNAs were extracted and then amplified by RT-PCR,the PCR products were cloned and sequenced.BioEdit 6.0.7 and MEGA 4.0 software were used to analyze gene sequences,calculate gene dispersion ratio and construct phylogenetic tree.Results The sequences of 13 specimens were successfully obtained.The HIV strains from T1,T2 and T3 were CRF07_BC recombinants,those from 5 out of the 6 controls lived in the same city with T2 and T3 were CRF07_BC recombinants as well,while those from 4 controls living in the same city with T1 were CRF01_AE recombinants.Compared with the clone sequences from T1,the mean gene dispersion ratio of T2 was the least (2.0％),followed by C12(2.8％),T3 (2.9％) and others.The phylogenetic tree showed that all clones from T1,T2,T3 and C12 might cluster together,and implied that the direction of HIV transmission was from T3 to T2,and then to T1.Conclusion The results support the possible epidemiological clue that HIV was transmitted from T3 to T2,and then to T1,indicating that molecular epidemiological investigation could provide more direct evidence for tracing postexposure HIV transmission.