CAJ | 학술논문

目的调查2011年黑龙江省黑瞎子岛鼠群中钩端螺旋体的感染情况.方法于2011年4-10月在黑龙江省黑瞎子岛采用夹夜法捕鼠,共捕鼠356只,无菌操作采集鼠肾样品；提取鼠肾脏总核酸,采用PCR方法,利用钩体鉴别引物,对提取的肾脏总核酸中23 S rDNA的482 bp特异性片段进行扩增,以检测鼠体钩端螺旋体感染情况；抽取不同月份的15份阳性扩增产物进行纯化,用双脱氧终止法测序,并利用Mega 4.0软件与Genebank中已知的国内外菌株基因序列进行比对及系统发育分析.结果在捕捉到的356只鼠中,红背(鼠平)和黑线姬鼠所占比例较大,分别为39.3％(140/356)和36.0％(128/356).所有鼠中共检测到钩端螺旋体阳性39只,感染率为11.0％,除褐家鼠外,其他鼠种均有感染,黑线姬鼠、红背鼾、东方田鼠、花鼠和大林姬鼠的感染率分别为9.4％ (12/128)、12.9％(18/140)、10.8％(7/65),差异无统计学意义(x2=1.92,P＞0.05).4月份未检测到感染疫鼠,5、6、7、8、9和10月份的感染率分别为5.6％(4/72)、8.8％(5/57)、12.8％(5/39)、9.8％(5/51)、33.3％(11/33)和22.5％ (9/40),差异有统计学意义(x2=32.92,P＜0.05)；黑瞎子岛鼠群感染的钩端螺旋体在同一进化支上,相似度为97.1％～99.6％,与澳大利亚的菌株U90865在进化上最为相近,相似度＞96.3％.结论该地区鼠群中已存在钩端螺旋体的感染,所感染钩体在进化上相似,且存在季节性差异,而对宿主的选择性不明显.
목적 조사2011년흑룡강성흑할자도서군중구단라선체적감염정황.방법 우2011년4-10월재흑룡강성흑할자도채용협야법포서,공포서356지,무균조작채집서신양품；제취서신장총핵산,채용PCR방법,이용구체감별인물,대제취적신장총핵산중23 S rDNA적482 bp특이성편단진행확증,이검측서체구단라선체감염정황；추취불동월빈적15빈양성확증산물진행순화,용쌍탈양종지법측서,병이용Mega 4.0연건여Genebank중이지적국내외균주기인서렬진행비대급계통발육분석.결과 재포착도적356지서중,홍배(서평)화흑선희서소점비례교대,분별위39.3％(140/356)화36.0％(128/356).소유서중공검측도구단라선체양성39지,감염솔위11.0％,제갈가서외,기타서충균유감염,흑선희서、홍배한、동방전서、화서화대림희서적감염솔분별위9.4％ (12/128)、12.9％(18/140)、10.8％(7/65),차이무통계학의의(x2=1.92,P＞0.05).4월빈미검측도감염역서,5、6、7、8、9화10월빈적감염솔분별위5.6％(4/72)、8.8％(5/57)、12.8％(5/39)、9.8％(5/51)、33.3％(11/33)화22.5％ (9/40),차이유통계학의의(x2=32.92,P＜0.05)；흑할자도서군감염적구단라선체재동일진화지상,상사도위97.1％～99.6％,여오대리아적균주U90865재진화상최위상근,상사도＞96.3％.결론 해지구서군중이존재구단라선체적감염,소감염구체재진화상상사,차존재계절성차이,이대숙주적선택성불명현.
Objective To study the infection status of Leptospira in rodents on Heixiazi island Heilongjiang province in 2011.Methods A total of 356 rodents were captured by night trap on the Heixiazi island from April to October 2011.The kidney tissue samples were collected by asepsis operation and the genomic DNA were extracted from them.Leptospira strains were confirmed by polymerase chain reaction (PCR) amplification of the 482 bp 23 S rDNA gene.Fifteen PCR products selected by the month were purified and sequenced by the methods of Sanger dideoxy,the sequences then compared with other Leptospira strains in Genebank,and phylogenetic analyses were drafted by software Mega 4.0.Results Among 356 rodents,the dominant species were Clethrionomys rutilus (39.3％,140/356) and Apodemus agrarius (36.0％,128/356).The infection rate of Leptospira was 11.0％,with 39 rodent samples detected positive.All the rodent species were infected except for Rattus norvegicus.The infection rate was 9.4％ (12/128) in Apodemus agrarius,12.9％ (18/140) in Clethrionomys rutilus,10.8％ (7/65) in Microtus fortis Buchner.No significant difference was found between the infection rate and the species of rodents by chi square test (x2 =1.92,P ＞ 0.05).Among months,the infection rate was 5.6％ (4/72) in May,8.8％ (5/57) in June,12.8％ (5/39) in July,9.8％ (5/51) in August,33.3％ (11/33) in September,22.5％ (9/40) in October,but no infection in April.There was significant difference in infection in different months (x2 =32.92,P ＜ 0.05).All the Leptospira in rodents on the Heixiazi island were in the same phylogenetic branch with a high similarity of 97.1％-99.6％,close with the Australia strain U90865 by the similarity above 96.3％.Conclusion Leptospira is probably prevalent in rodents on the Heixiazi island,and the phylogene of the strains were similar.The infection rate in rodents was significantly different in months but not in hosts.