CAJ | 학술논문

目的调查2012年河南省丙型肝炎病毒(HCV)感染状况和基因型分布.方法采用多阶段随机抽样的方法,于2012年3-6月在河南省抽取1～74岁常住人群32 203名,对其进行问卷调查,收集一般人口学信息、既往病史以及危险因素的暴露史等.采集5 ml静脉血,经ELISA和巢式RT-PCR法分别进行HCV抗体和HCV RNA检测；并对HCV RNA阳性样本进行非结构蛋白5区(NS5B)基因扩增和测序,对所测序列进行系统进化树分析和基因分型；分析不同特征调查对象抗-HCV、HCV-RNA阳性率及HCV基因亚型分布的差异.结果在32 203名调查对象中,抗-HCV和HCV RNA阳性率分别为0.48％ (153/32 203)和0.24％ (78/32 203),其中男性分别为0.42％ (65/15 634)和0.23％ (36/15 634),女性分别为0.53％ (88/16 569)和0.25％ (42/16 596),性别间阳性率差异均无统计学意义(x2值分别为2.26和0.18,P值均>0.05).NS5B基因分型与分子进化分析结果显示,在71份HCV RNA阳性样本中,共有6种亚型,分别为lb、6a、3a、2a、3b和1a,所占比例分别为56.3％ (40/71)、19.7％(14/71)、11.3％ (8/71)、8.5％ (6/71)、2.8％(2/71)和1.4％(1/71)；感染者HCV基因亚型主要集中在lb和6a这两个分支上,两种亚型的Bootstrap值均为0.95.结论 2012年河南地区HCV流行处于较高水平,基因型亚型以1b和6a型为主.
목적 조사2012년하남성병형간염병독(HCV)감염상황화기인형분포.방법 채용다계단수궤추양적방법,우2012년3-6월재하남성추취1～74세상주인군32 203명,대기진행문권조사,수집일반인구학신식、기왕병사이급위험인소적폭로사등.채집5 ml정맥혈,경ELISA화소식RT-PCR법분별진행HCV항체화HCV RNA검측；병대HCV RNA양성양본진행비결구단백5구(NS5B)기인확증화측서,대소측서렬진행계통진화수분석화기인분형；분석불동특정조사대상항-HCV、HCV-RNA양성솔급HCV기인아형분포적차이.결과 재32 203명조사대상중,항-HCV화HCV RNA양성솔분별위0.48％ (153/32 203)화0.24％ (78/32 203),기중남성분별위0.42％ (65/15 634)화0.23％ (36/15 634),녀성분별위0.53％ (88/16 569)화0.25％ (42/16 596),성별간양성솔차이균무통계학의의(x2치분별위2.26화0.18,P치균>0.05).NS5B기인분형여분자진화분석결과현시,재71빈HCV RNA양성양본중,공유6충아형,분별위lb、6a、3a、2a、3b화1a,소점비례분별위56.3％ (40/71)、19.7％(14/71)、11.3％ (8/71)、8.5％ (6/71)、2.8％(2/71)화1.4％(1/71)；감염자HCV기인아형주요집중재lb화6a저량개분지상,량충아형적Bootstrap치균위0.95.결론 2012년하남지구HCV류행처우교고수평,기인형아형이1b화6a형위주.
Objective To investigate the prevalence and distribution of hepatitis C virus (HCV) genotypes in Henan province in 2012.Methods A total of 32 203 permanent residents (1 to 74 years old) in Henan were recruited using multi-stage random samping method from March to June 2012.All participants were asked to complete a questionnaire to collect demographic information,past medical history and the exposure history of risk factors.A blood sample of 5 ml was collected at the same time.The condition of anti-HCV and HCV RNA was determined through the ELISA test and nested RT-PCR.HCV RNA positive samples were further subject to the nonstmctural protein 5 region (NSSB) gene amplification and sequencing.The sequence was amplified for the phylogenetic tree and genetic analysis.The differences of the positive rate of anti-HCV and HCV RNA and the HCV genetic subtype distribution in different respondents' characteristics were analyzed.Results Among 32 203 subjects,the overall positive rate of anti-HCV and HCV RNA were 0.48％ (153/32 203) and 0.24％ (78/32 203),in which men were 0.42％ (65/15 634),and0.23％ (36/15 634),and women were 0.53％ (88/16 569) and 0.25％ (42/16 596).The differences between men and women were not statistically significant (x2 values were 2.26,0.18,respectively,both P values ＞ 0.05).The results of NS5B genotyping and molecular evolution analysis showed that there were six subtypes in the 71 HCV RNA positive samples.In those six subtypes,the proportion of genotypes lb,6a,3a,2a,3b and la were 56.3％ (40/71),19.7％ (14/71),11.3％ (8/71),8.5％ (6/71),2.8％ (2/71)and 1.4％ (1/71),respectively.The HCV genetic subtypes of infestor were mainly present with two branches of 1 b and 6a,and the two subtypes Bootstrap values were 0.95.Conclusion The prevalence of HCV infection was high in Henan.The major HCV genotypes in patients with HCV infection were 1b and 6a.