中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2013年
6期
556-560
,共5页
黄夏宁%张永路%叶晓艳%肖雯晴%钟晴%谷康定
黃夏寧%張永路%葉曉豔%肖雯晴%鐘晴%穀康定
황하저%장영로%협효염%초문청%종청%곡강정
微囊藻毒素类%小鼠%单核细胞%淋巴细胞%细胞因子类
微囊藻毒素類%小鼠%單覈細胞%淋巴細胞%細胞因子類
미낭조독소류%소서%단핵세포%림파세포%세포인자류
Microcystins%Mice%Monocytes%Lymphocytes%Cytokines
目的 探讨微囊藻毒素-LR(MC-LR)对小鼠血液中单核细胞和淋巴细胞的影响及毒性效应,筛查血液中早期灵敏效应指标.方法 1月龄SPF级雄性昆明小鼠按体重采用随机数字表法分为5组,每组7只,分别分为对照组、低剂量组、中低剂量组、中高剂量组、高剂量组,每天给予1次腹腔注射染毒,分别给予生理盐水及3.125、6.250、12.500、25.000 μg/kg剂量的MC-LR.染毒7d后采用放射免疫方法检测重要相关细胞因子,KCl-SDS沉淀法检测淋巴细胞DNA-蛋白质交联(DNAprotein crosslinks,DPC)情况,流式细胞仪检测单核细胞吞噬功能及单核细胞和淋巴细胞的活性氧变化(reactive oxygen species,ROS),并分析其变化情况.结果 中低、中高和高剂量MC-LR染毒组小鼠白细胞介素-6[分别为(346.837±25.536)、(360.847±37.886)、(434.245±35.858) pg/ml]均高于对照组[(232.775±32.816) pg/ml](t值分别为-7.258、-6.760、-10.966,P值均＜0.05)；高剂量组肿瘤坏死因子-α含量[(10.782±0.966) fmol/ml]低于对照组[(16.878±3.378) fmol/ml](t=4.591,P＜0.05).中低和高剂量组淋巴细胞DPC[分别为(242.576±7.545)、(241.472±2.793) ng/ml]高于对照组[(228.657±4.130) ng/ml](t值分别为-4.282、-6.801,P值均＜0.05)；低、中低、中高和高剂量组淋巴细胞DCF荧光强度(依次为3299.37±120.54、3281.38±58.34、3308.06±136.12、3346.92±108.69)均低于对照组(3770.81±131.39)(t值分别为6.995、9.007、6.472、6.577,P值均＜0.05).低、中低、中高和高剂量单核细胞DCF荧光强度(依次为3271.51±140.79、3270.05±117.92、3326.90±114.39、3292.49±145.97)均低于对照组(3841.72±130.92)(t值分别为7.847、8.584、7.835、7.411,P值均＜0.05).其他检测指标未见明显改变.结论 在体内实验中,可观察到MC-LR对血液中单核细胞和淋巴细胞有一定的影响.经比较,白细胞ROS为本实验中最灵敏效应指标.
目的 探討微囊藻毒素-LR(MC-LR)對小鼠血液中單覈細胞和淋巴細胞的影響及毒性效應,篩查血液中早期靈敏效應指標.方法 1月齡SPF級雄性昆明小鼠按體重採用隨機數字錶法分為5組,每組7隻,分彆分為對照組、低劑量組、中低劑量組、中高劑量組、高劑量組,每天給予1次腹腔註射染毒,分彆給予生理鹽水及3.125、6.250、12.500、25.000 μg/kg劑量的MC-LR.染毒7d後採用放射免疫方法檢測重要相關細胞因子,KCl-SDS沉澱法檢測淋巴細胞DNA-蛋白質交聯(DNAprotein crosslinks,DPC)情況,流式細胞儀檢測單覈細胞吞噬功能及單覈細胞和淋巴細胞的活性氧變化(reactive oxygen species,ROS),併分析其變化情況.結果 中低、中高和高劑量MC-LR染毒組小鼠白細胞介素-6[分彆為(346.837±25.536)、(360.847±37.886)、(434.245±35.858) pg/ml]均高于對照組[(232.775±32.816) pg/ml](t值分彆為-7.258、-6.760、-10.966,P值均＜0.05)；高劑量組腫瘤壞死因子-α含量[(10.782±0.966) fmol/ml]低于對照組[(16.878±3.378) fmol/ml](t=4.591,P＜0.05).中低和高劑量組淋巴細胞DPC[分彆為(242.576±7.545)、(241.472±2.793) ng/ml]高于對照組[(228.657±4.130) ng/ml](t值分彆為-4.282、-6.801,P值均＜0.05)；低、中低、中高和高劑量組淋巴細胞DCF熒光彊度(依次為3299.37±120.54、3281.38±58.34、3308.06±136.12、3346.92±108.69)均低于對照組(3770.81±131.39)(t值分彆為6.995、9.007、6.472、6.577,P值均＜0.05).低、中低、中高和高劑量單覈細胞DCF熒光彊度(依次為3271.51±140.79、3270.05±117.92、3326.90±114.39、3292.49±145.97)均低于對照組(3841.72±130.92)(t值分彆為7.847、8.584、7.835、7.411,P值均＜0.05).其他檢測指標未見明顯改變.結論 在體內實驗中,可觀察到MC-LR對血液中單覈細胞和淋巴細胞有一定的影響.經比較,白細胞ROS為本實驗中最靈敏效應指標.
목적 탐토미낭조독소-LR(MC-LR)대소서혈액중단핵세포화림파세포적영향급독성효응,사사혈액중조기령민효응지표.방법 1월령SPF급웅성곤명소서안체중채용수궤수자표법분위5조,매조7지,분별분위대조조、저제량조、중저제량조、중고제량조、고제량조,매천급여1차복강주사염독,분별급여생리염수급3.125、6.250、12.500、25.000 μg/kg제량적MC-LR.염독7d후채용방사면역방법검측중요상관세포인자,KCl-SDS침정법검측림파세포DNA-단백질교련(DNAprotein crosslinks,DPC)정황,류식세포의검측단핵세포탄서공능급단핵세포화림파세포적활성양변화(reactive oxygen species,ROS),병분석기변화정황.결과 중저、중고화고제량MC-LR염독조소서백세포개소-6[분별위(346.837±25.536)、(360.847±37.886)、(434.245±35.858) pg/ml]균고우대조조[(232.775±32.816) pg/ml](t치분별위-7.258、-6.760、-10.966,P치균＜0.05)；고제량조종류배사인자-α함량[(10.782±0.966) fmol/ml]저우대조조[(16.878±3.378) fmol/ml](t=4.591,P＜0.05).중저화고제량조림파세포DPC[분별위(242.576±7.545)、(241.472±2.793) ng/ml]고우대조조[(228.657±4.130) ng/ml](t치분별위-4.282、-6.801,P치균＜0.05)；저、중저、중고화고제량조림파세포DCF형광강도(의차위3299.37±120.54、3281.38±58.34、3308.06±136.12、3346.92±108.69)균저우대조조(3770.81±131.39)(t치분별위6.995、9.007、6.472、6.577,P치균＜0.05).저、중저、중고화고제량단핵세포DCF형광강도(의차위3271.51±140.79、3270.05±117.92、3326.90±114.39、3292.49±145.97)균저우대조조(3841.72±130.92)(t치분별위7.847、8.584、7.835、7.411,P치균＜0.05).기타검측지표미견명현개변.결론 재체내실험중,가관찰도MC-LR대혈액중단핵세포화림파세포유일정적영향.경비교,백세포ROS위본실험중최령민효응지표.
Objective To investigate the influence of microcystin-LR (MC-LR) on monocytes and lymphocytes in blood of mice and to find a sensitive index of toxic effects.Methods Specific pathogen free Kunming male mice,aging 1 month-old,were randomly divided into 5 groups by weights,7 mice for each group.The mice in 5 groups were exposed to MC-LR through intraperitoneal injection at 0,3.125,6.250,12.500 and 25.000 μg/kg respectively for 7 days.Then cytokine levels in the serum were measured by radioimmunoassay,DNA-protein crosslinks (DPC) was measured by the SDS/KC1 precipitation technique,and the phagocytosis and ROS of leukocytes were detected by flow cytometry.Results The levels of interleukin 6 in the 6.250,12.500 and 25.000 μg · kg-1 · d-1 dose groups were (346.837 ± 25.536),(360.847 ± 37.886) and (434.245 ± 35.858) pg/ml respectively,which were significantly higher than those in the control group which the value was (232.775 ± 32.816) pg/ml (t values were-7.258,-6.760 and-10.966 respectively,P values were all ＜ 0.05).While the level of tumor necrosis factoralpha was (10.782 ±0.966) fmol/ml in 25 μg · kg-1 · d-1 dose group was statistically lower than it in the control group which the value was (16.878 ± 3.378) fmol/ml (t value was 4.591,P ＜ 0.05).The DPC levels of lymphocytes in 6.250,12.500 μg · kg-1 · d-1 dose group were (242.576 ± 7.545),(241.472 ±2.793) ng/ml,higher than it in the control group while the value was (228.657 ±4.130) ng/ml (t value was-4.282,-6.801,P values were all ＜0.05).The fluorescence intensity of DCF in lymphocytes in the 4 treated groups were separately 3299.37 ± 120.54,3281.38 ± 58.34,3308.06 ± 136.12 and 3346.92 ±108.69,all significantly lower than 3770.81 ± 131.39 in the control group (t values were 6.995,9.007,6.472 and 6.577 respectively,and P values were all ＜0.05).The fluorescence intensity of DCF in monocytes in the 4 treated groups (3271.51 ± 140.79,3270.05 ± 117.92,3326.90 ± 114.39 and 3292.49 ± 145.97 respectively) were also significantly lower than the value in the control group was 3841.72 ± 130.92 (t values were 7.847,8.584,7.835 and 7.411 respectively,P values were all ＜0.05).There was no significant difference in other index among the four experiment groups and the control group.Conclusion The MC-LR administered via intraperitoneal injection to mice induced the alterations of some cytokines of monocytes and lymphocytes in blood.By comparison,the ROS of leukocyte was the most sensitive index.
