CAJ | 학술논문

目的了解2005-2011年河南省小肠结肠炎耶尔森菌分布情况.方法于2005-2011年在监测点郑州、睢县和登封采集腹泻患者粪便标本,各种家禽家畜的粪便标本,苍蝇,以及生、熟肉制品涂抹标本,共6700份.采用冷增菌方法进行目的菌分离.对不同宿主样品中分离到的小肠结肠炎耶尔森菌进行系统生化鉴定、血清学分型、生物分型和毒力基因PCR检测.结果从11种常见宿主和食品中共分离到216株小肠结肠炎耶尔森菌,29.6％ (64/216)分离自猪.所分离的小肠结肠炎耶尔森菌主要流行的血清型是0∶5和0∶8,所占比例分别为23.2％(50/216)和20.4％(44/216)；生物分型以1A型为主,占84.7％ (183/216)；不同宿主的优势血清型及生物分型各不相同；猪分离株中致病性菌株最多,为16株,其次是腹泻患者和犬分离株,均为6株.结论河南省小肠结肠炎耶尔森菌宿主分布广泛,猪是主要宿主.
목적 료해2005-2011년하남성소장결장염야이삼균분포정황.방법 우2005-2011년재감측점정주、휴현화등봉채집복사환자분편표본,각충가금가축적분편표본,창승,이급생、숙육제품도말표본,공6700빈.채용랭증균방법진행목적균분리.대불동숙주양품중분리도적소장결장염야이삼균진행계통생화감정、혈청학분형、생물분형화독력기인PCR검측.결과 종11충상견숙주화식품중공분리도216주소장결장염야이삼균,29.6％ (64/216)분리자저.소분리적소장결장염야이삼균주요류행적혈청형시0∶5화0∶8,소점비례분별위23.2％(50/216)화20.4％(44/216)；생물분형이1A형위주,점84.7％ (183/216)；불동숙주적우세혈청형급생물분형각불상동；저분리주중치병성균주최다,위16주,기차시복사환자화견분리주,균위6주.결론 하남성소장결장염야이삼균숙주분포엄범,저시주요숙주.
Objective To investigate the distribution of Yersinia enterocolitica in Henan province from 2005 to 2011.Methods A total of 6700 samples of stool specimen were collected from diarrhea patients and different domestic animals between 2005 and 2011 from Zhengzhou,Suixian and Dengfeng,as well as flies and the daub specimens of raw and cooked meat products.The bacteria were isolated by cold enrichment method,analyzed by the systematic biochemistry to determine the serotypes and bio-types,and tested the virulence genes by PCR method.Results A total of 216 strains of Yersinia enterocolitica were isolated from 11 kinds of animal hosts and foods,while 29.63％ (64/216) of them were from swine.The dominant epidemic serotypes of the Yersinia enterocolitica were O:5 and O:8,accounted for 23.2％ (50/216) and 20.4％ (44/216),respectively; type 1A was the dominant bio-type,accounted for 84.7％ (183/216).The dominant serotype and bio-type differed a lot among various hosts.16 pathogenic strains were isolated from swine,followed by diarrhea patients (6 strains) and dogs (6 strains).Conclusion The distribution of the host of Yersinia enterocolitica was widespread,while swine was the dominant animal host.