中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2013年
8期
741-746
,共6页
王甜%邓棋霏%张笑%李晓亮%邓思芸%戴霞云%黄素丽%冯晶%李珺
王甜%鄧棋霏%張笑%李曉亮%鄧思蕓%戴霞雲%黃素麗%馮晶%李珺
왕첨%산기비%장소%리효량%산사예%대하운%황소려%풍정%리군
多态性,单核苷酸%微核%焦炉工%miR-146a%miR-196a2
多態性,單覈苷痠%微覈%焦爐工%miR-146a%miR-196a2
다태성,단핵감산%미핵%초로공%miR-146a%miR-196a2
Polymorphism,single nucleotide%Micronuclei%Coke oven workers%miR-146a%miR-196a2
目的 探讨miR-146a和miR-196a2前体区遗传多态位点rs2910164 G＞C和rs11614913 T＞C与焦炉工遗传损伤的关联性.方法 于2010年9月至10月,选取湖北省武汉市某焦化厂工龄在1年以上,无肿瘤,无吸烟习惯的焦炉作业工人,共575名.收集一般人口学资料并采集外周静脉血和尿液；采用胞质分裂阻滞试验计算外周血淋巴细胞的微核率来评价染色体损伤的程度；采用TaqMan探针对miR-146a前体区rs2910164 G＞C和miR-196a2前体区rs11614913 T＞C多态位点进行分型分析；采用三明治ELISA测定血浆中苯并[a]芘二氢二醇环氧化物-白蛋白(BPDE-白蛋白)加合物的浓度；并进行数据分析,并计算频率比(FR)及其95％ CI值.结果 共纳入575名工人.与携带野生型rs2910164 GG基因型的工人的外周血淋巴细胞微核率(4.02±3.09)相比,携带rs2910164CC基因型工人的微核率(4.38±3.46)增加(FR=1.18,95％CI:1.04～1.34),且有明显的等位基因剂量-效应关系(P趋势=0.025).按研究对象不同特征分层后,发现男性(FR=1.11,95％ CI:1.02～ 1.20)、不饮酒(FR=1.07,95％ CI:1.00 ～1.14)、工龄＜20年(FR=1.12,95％CI:1.03 ～ 1.22)和低BPDE-白蛋白水平组(FR=1.11,95％CI:1.02～1.21)中,rs2910164C等位基因与微核率的增加具有等位基因剂量-效应关系(P趋势分别为0.011、0.044、0.006和0.020).此外,与携带rs11614913TT基因型的工人(3.90±3.32)相比,携带rs11614913 TC基因型工人的外周血淋巴细胞微核率增加(4.27±2.91)(FR=1.12,95％CI:1.02 ～ 1.23).以同时携带rs2910164 GG及rs11614913TT基因型的工人组作为对照,同时携带rs2910164CC与rs11614913 CC基因型个体的外周血淋巴细胞微核率(5.32±4.94)是对照组(3.75±3.01)的1.51(1.21 ～1.89)倍.结论 miR-146a前体区域rs2910164C等位基因及miR-196a2前体区域rs11614913 C等位基因与焦炉工遗传损伤水平增加有关.
目的 探討miR-146a和miR-196a2前體區遺傳多態位點rs2910164 G＞C和rs11614913 T＞C與焦爐工遺傳損傷的關聯性.方法 于2010年9月至10月,選取湖北省武漢市某焦化廠工齡在1年以上,無腫瘤,無吸煙習慣的焦爐作業工人,共575名.收集一般人口學資料併採集外週靜脈血和尿液；採用胞質分裂阻滯試驗計算外週血淋巴細胞的微覈率來評價染色體損傷的程度；採用TaqMan探針對miR-146a前體區rs2910164 G＞C和miR-196a2前體區rs11614913 T＞C多態位點進行分型分析；採用三明治ELISA測定血漿中苯併[a]芘二氫二醇環氧化物-白蛋白(BPDE-白蛋白)加閤物的濃度；併進行數據分析,併計算頻率比(FR)及其95％ CI值.結果 共納入575名工人.與攜帶野生型rs2910164 GG基因型的工人的外週血淋巴細胞微覈率(4.02±3.09)相比,攜帶rs2910164CC基因型工人的微覈率(4.38±3.46)增加(FR=1.18,95％CI:1.04～1.34),且有明顯的等位基因劑量-效應關繫(P趨勢=0.025).按研究對象不同特徵分層後,髮現男性(FR=1.11,95％ CI:1.02～ 1.20)、不飲酒(FR=1.07,95％ CI:1.00 ～1.14)、工齡＜20年(FR=1.12,95％CI:1.03 ～ 1.22)和低BPDE-白蛋白水平組(FR=1.11,95％CI:1.02～1.21)中,rs2910164C等位基因與微覈率的增加具有等位基因劑量-效應關繫(P趨勢分彆為0.011、0.044、0.006和0.020).此外,與攜帶rs11614913TT基因型的工人(3.90±3.32)相比,攜帶rs11614913 TC基因型工人的外週血淋巴細胞微覈率增加(4.27±2.91)(FR=1.12,95％CI:1.02 ～ 1.23).以同時攜帶rs2910164 GG及rs11614913TT基因型的工人組作為對照,同時攜帶rs2910164CC與rs11614913 CC基因型箇體的外週血淋巴細胞微覈率(5.32±4.94)是對照組(3.75±3.01)的1.51(1.21 ～1.89)倍.結論 miR-146a前體區域rs2910164C等位基因及miR-196a2前體區域rs11614913 C等位基因與焦爐工遺傳損傷水平增加有關.
목적 탐토miR-146a화miR-196a2전체구유전다태위점rs2910164 G＞C화rs11614913 T＞C여초로공유전손상적관련성.방법 우2010년9월지10월,선취호북성무한시모초화엄공령재1년이상,무종류,무흡연습관적초로작업공인,공575명.수집일반인구학자료병채집외주정맥혈화뇨액；채용포질분렬조체시험계산외주혈림파세포적미핵솔래평개염색체손상적정도；채용TaqMan탐침대miR-146a전체구rs2910164 G＞C화miR-196a2전체구rs11614913 T＞C다태위점진행분형분석；채용삼명치ELISA측정혈장중분병[a]비이경이순배양화물-백단백(BPDE-백단백)가합물적농도；병진행수거분석,병계산빈솔비(FR)급기95％ CI치.결과 공납입575명공인.여휴대야생형rs2910164 GG기인형적공인적외주혈림파세포미핵솔(4.02±3.09)상비,휴대rs2910164CC기인형공인적미핵솔(4.38±3.46)증가(FR=1.18,95％CI:1.04～1.34),차유명현적등위기인제량-효응관계(P추세=0.025).안연구대상불동특정분층후,발현남성(FR=1.11,95％ CI:1.02～ 1.20)、불음주(FR=1.07,95％ CI:1.00 ～1.14)、공령＜20년(FR=1.12,95％CI:1.03 ～ 1.22)화저BPDE-백단백수평조(FR=1.11,95％CI:1.02～1.21)중,rs2910164C등위기인여미핵솔적증가구유등위기인제량-효응관계(P추세분별위0.011、0.044、0.006화0.020).차외,여휴대rs11614913TT기인형적공인(3.90±3.32)상비,휴대rs11614913 TC기인형공인적외주혈림파세포미핵솔증가(4.27±2.91)(FR=1.12,95％CI:1.02 ～ 1.23).이동시휴대rs2910164 GG급rs11614913TT기인형적공인조작위대조,동시휴대rs2910164CC여rs11614913 CC기인형개체적외주혈림파세포미핵솔(5.32±4.94)시대조조(3.75±3.01)적1.51(1.21 ～1.89)배.결론 miR-146a전체구역rs2910164C등위기인급miR-196a2전체구역rs11614913 C등위기인여초로공유전손상수평증가유관.
Objective To investigate the association of rs2910164 G ＞ C polymorphism and rs11614913 T ＞ C polymorphism in pre-miR-146a and pre-miR-196a2 with genetic damage levels in coke oven workers.Methods A total of 575 nonsmoking workers who have worked for more than one year in a coke-oven plant at Wuhan,Hubei Province were enrolled in this study in September to October,2010.The general characteristics as well as blood and urine samples were collected.The genetic damage levels were detected by cytokinesis-block micronucleus cytome assay and represented as micronucleus (MN) frequencies of binucleate cells in peripheral blood lymphocytes.The rs2910164 G ＞ C polymorphisms in pre-miR-146a and rs11614913 T ＞ C polymorphisms in pre-miR-196a2 were genotyped by using TaqMan assay.The plasma concentrations of benzo [a] pyrene-diolepoxide (BPDE)-albumin adducts were determined by using ELISA.All data were analyzed,the frequency ratio (FR) and 95％ CI were calculated.Results Totally,575 workers were taken into consideration.The rs2910164 C allele was associated with increased MN frequencies in the coke oven workers (Ptrend =0.025),and the MN frequencies were higher in rs2910164CC genotype carriers (4.38 ± 3.46) than in wild-type rs2910164 GG genotype carriers (4.02 ± 3.09)(FR =1.18,95％ CI:1.04-1.34).The further stratified analyses by working years,gender,alcohol consumption,and the levels of BPDE-albumin adducts showed that the effects of rs2910164 C allele in increasing MN frequencies were robust in subjects who were males (FR =1.11,95％ CI:1.02-1.20),nondrinkers (FR =1.07,95％ CI:1.00-1.14),working years less than 20 (FR =1.12,95％ CI:1.03-1.22),and workers with lower BPDE-albumin adducts levels (FR =1.11,95％ CI:1.02-1.21) (Ptrend =0.011,0.044,0.006 and 0.020,respectively).In addition,the MN frequencies were higher in workers with rs11614913 TC genotype (4.27 ± 2.91) than workers with rs11614913 TT genotype (3.90 ± 3.32)(FR =1.12,95％ CI:1.02-1.23).Workers carried both rs2910164 GG and rs11614913 TT genotypes were set as a control,and the MN frequencies of workers with both rs2910164 CC and rs11614913 CC genotypes (5.32 ± 4.94) were 1.51 (1.21-1.89) times higher than the control (3.75 ± 3.01).Conclusion The rs2910164 C allele in pre-miR-146a and rs11614913 C allele in pre-miR-196a2 were associated with increased genetic damage levels in coke oven workers.
