CAJ | 학술논문

目的调查2009-2011年深圳市头孢曲松低敏淋病奈瑟菌(淋球菌)流行的基因型和特征.方法收集2009至201 1年深圳市淋球菌296株,采用琼脂稀释法筛选出头孢曲松低敏株(最小抑菌浓度为0.06 ～0.50 μg/ml),用logistic回归分析方法,分析与头孢曲松低敏淋球菌感染相关的因素；根据1∶1抽样原则抽取出高敏菌株,与低敏菌株一起进行淋病奈瑟菌抗原序列分析(NG-MAST)基因分型和邻接法(neighbor-joining,NJ)进化树分析.结果在296株淋球菌中,未发现头孢曲松耐药菌株,头孢曲松低敏菌株53株(17.9％).近2个月的抗生素使用史与头孢曲松低敏淋球菌感染相关(OR=3.080,95％ CI:1.376 ～6.894).53株低敏株的NG-MAST共得到48个ST型,由5个成簇的ST(ST1768、ST3927、ST641、ST7076、ST7078各包含了2株淋球菌)和43个单一的ST构成,其中26个ST型中国香港等地曾有报道.NJ进化树未观察到低敏株单独成簇的情况.结论深圳市2009-2011年分离到的296株淋球菌中头孢曲松低敏株的比例较高；深圳市头孢曲松低敏淋球菌菌株的流行可能有其独特的特点.
목적 조사2009-2011년심수시두포곡송저민임병내슬균(림구균)류행적기인형화특정.방법 수집2009지201 1년심수시림구균296주,채용경지희석법사선출두포곡송저민주(최소억균농도위0.06 ～0.50 μg/ml),용logistic회귀분석방법,분석여두포곡송저민림구균감염상관적인소；근거1∶1추양원칙추취출고민균주,여저민균주일기진행임병내슬균항원서렬분석(NG-MAST)기인분형화린접법(neighbor-joining,NJ)진화수분석.결과 재296주림구균중,미발현두포곡송내약균주,두포곡송저민균주53주(17.9％).근2개월적항생소사용사여두포곡송저민림구균감염상관(OR=3.080,95％ CI:1.376 ～6.894).53주저민주적NG-MAST공득도48개ST형,유5개성족적ST(ST1768、ST3927、ST641、ST7076、ST7078각포함료2주림구균)화43개단일적ST구성,기중26개ST형중국향항등지증유보도.NJ진화수미관찰도저민주단독성족적정황.결론 심수시2009-2011년분리도적296주림구균중두포곡송저민주적비례교고；심수시두포곡송저민림구균균주적류행가능유기독특적특점.
Objective To investigate the genotype and characteristics of Neisseria gonorrhoeae (N.gonorrhoeae) isolates with reduced susceptibility to ceftriaxone in Shenzhen from 2009 to 2011.Methods A total of 296 N.gonorrhoeae isolates were collected in Shenzhen from 2009 to 2011.ceftriaxone strains (minimum inhibitory concentration between 0.06 and 0.50 μg/ml) were determined by agar dilution method.Logistic regression was used to analyze the associated factors of ceftriaxone N.gonorrhoeae infection.Neighbor-joining(NJ) phylogenetic tree analysis and N.gonorrhoeae multiantigen sequence typing (NG-MAST) were performed on all ceftriaxone isolates and susceptible control isolates randomly selected in accordance with the principle of 1 ∶ 1 sampling.Results No isolates displayed resistance to ceftriaxone,whereas 53 (17.9％) showed reduced susceptibility to ceftriaxone among 296 isolates.Only antibiotic use in recent two months was associated with ceftriaxone isolates infection(OR =3.080,95％ CI:1.376-6.894).Among the ceftriaxone isolates,48 different ST were identified including 5 STs (ST1768,ST3927,ST641,ST7076 and ST7078) containing 2 isolates and 43 single STs.There were 26 STs previously reported from HongKong in China.Low sensitive strains clustering was not observed by NJ phylogenetic tree.Conclusion The proportion of ceftriaxone strains among the 296 N.gonorrhoeae isolates collected from 2009 to 2011 in Shenzhen is high.The STs of ceftriaxone strains may have unique epidemic features in Shenzhen.