中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2013年
12期
1100-1104
,共5页
胡逢蛟%李永东%焦素黎%张姝
鬍逢蛟%李永東%焦素黎%張姝
호봉교%리영동%초소려%장주
流感病毒属B型%血凝素类%神经氨酸酶%基因%突变
流感病毒屬B型%血凝素類%神經氨痠酶%基因%突變
류감병독속B형%혈응소류%신경안산매%기인%돌변
Influenza B virus%Hemagglutinins%Neuraminidase%Genes%Mutation
目的 分析2010-2012年宁波市乙型流行性感冒(简称流感)病毒的流行状态及表面抗原基因血凝素(HA)和神经氨酸酶(NA)进化情况.方法 收集宁波市2010-2012年流感样患者的咽拭子和含漱液标本3440份,进行流感病毒分离鉴定;同时采集2010年正常人群血清628份,进行抗体滴度测定.选择不同时期乙型流感分离株进行HA和NA基因序列测定,分析病毒表面抗原基因的进化方向与流行规律的关系.结果 共计109份临床样本分离出乙型流感病毒,其中Victoria系102份(93.6％),Yamagata系7份(6.4％);在628份正常人群血清中,Victoria和Yamagata系乙型流感病毒抗体阳性率分别为321 (51.1％)和300 (47.8％),差异无统计学意义(x2=1.405,P＞0.05).进化分析显示,2010-2012年Victoria系HA基因均聚集在疫苗株Malaysia/2506/2004分支中,而NA基因则出现了不同方向的进化分支.Victoria系流行株与Brisbane/60/2008在HA基因中仅1～5个氨基酸位点的差异,而NA基因上有6～16个位点的差异.相比HA基因,Victoria系NA的进化速度更快.结论 2010-2012年宁波市Victoria系乙型流感的NA基因进化速度比HA基因快;综合HA与NA基因序列分析对观察流感病毒的基因变异更为灵敏可靠.
目的 分析2010-2012年寧波市乙型流行性感冒(簡稱流感)病毒的流行狀態及錶麵抗原基因血凝素(HA)和神經氨痠酶(NA)進化情況.方法 收集寧波市2010-2012年流感樣患者的嚥拭子和含漱液標本3440份,進行流感病毒分離鑒定;同時採集2010年正常人群血清628份,進行抗體滴度測定.選擇不同時期乙型流感分離株進行HA和NA基因序列測定,分析病毒錶麵抗原基因的進化方嚮與流行規律的關繫.結果 共計109份臨床樣本分離齣乙型流感病毒,其中Victoria繫102份(93.6％),Yamagata繫7份(6.4％);在628份正常人群血清中,Victoria和Yamagata繫乙型流感病毒抗體暘性率分彆為321 (51.1％)和300 (47.8％),差異無統計學意義(x2=1.405,P＞0.05).進化分析顯示,2010-2012年Victoria繫HA基因均聚集在疫苗株Malaysia/2506/2004分支中,而NA基因則齣現瞭不同方嚮的進化分支.Victoria繫流行株與Brisbane/60/2008在HA基因中僅1～5箇氨基痠位點的差異,而NA基因上有6～16箇位點的差異.相比HA基因,Victoria繫NA的進化速度更快.結論 2010-2012年寧波市Victoria繫乙型流感的NA基因進化速度比HA基因快;綜閤HA與NA基因序列分析對觀察流感病毒的基因變異更為靈敏可靠.
목적 분석2010-2012년저파시을형류행성감모(간칭류감)병독적류행상태급표면항원기인혈응소(HA)화신경안산매(NA)진화정황.방법 수집저파시2010-2012년류감양환자적인식자화함수액표본3440빈,진행류감병독분리감정;동시채집2010년정상인군혈청628빈,진행항체적도측정.선택불동시기을형류감분리주진행HA화NA기인서렬측정,분석병독표면항원기인적진화방향여류행규률적관계.결과 공계109빈림상양본분리출을형류감병독,기중Victoria계102빈(93.6％),Yamagata계7빈(6.4％);재628빈정상인군혈청중,Victoria화Yamagata계을형류감병독항체양성솔분별위321 (51.1％)화300 (47.8％),차이무통계학의의(x2=1.405,P＞0.05).진화분석현시,2010-2012년Victoria계HA기인균취집재역묘주Malaysia/2506/2004분지중,이NA기인칙출현료불동방향적진화분지.Victoria계류행주여Brisbane/60/2008재HA기인중부1～5개안기산위점적차이,이NA기인상유6～16개위점적차이.상비HA기인,Victoria계NA적진화속도경쾌.결론 2010-2012년저파시Victoria계을형류감적NA기인진화속도비HA기인쾌;종합HA여NA기인서렬분석대관찰류감병독적기인변이경위령민가고.
Objective To investigate the epidemiological characteristics of influenza B viruses and explore the genetic evolution characteristics of the hemagglutinin (HA) and neuraminidase (NA) genes of local isolated strains in Ningbo,Southeast China,during 2010 to 2012.Methods Respiratory specimens from 3440 cases of patients with influenza-like illness(ILI) during 2010 to 2012 were collected in for virus isolation.And the 628 sera samples were collected in 2010 from the healthy community population to detect the HI antibody level against the local isolated virus.For phylogenetic analysis,the fragments of HA and NA genes were amplified and sequenced from strains isolated in different years.The association between evolution of HA and epidemiological characteristics were analyzed.Results A total of 109 strains of influenza B virus were isolated,including 102 (93.6％) Victoria-lineage strains and 7 (6.4％) Yamagata-lineage strains.Positive rates of HI antibody against Victoria-lineage strains and Yamagata-lineage strains were 51.1％ (321) and 47.8％ (300),respectively (x2 =1.405,P ＞ 0.05) among the 628 sera samples.The phylogenetic analysis showed that all HA fragments of isolated strains clustered the same branch with Malaysia/2506/2004 while the NA genes formed different branches.Compared with Brisbane/60/2008 strain,there were 1 to 5 Amino acid mutations in HA domain,and more mutations were detected in NA domain,ranged from 6 to 16 sites.The genetic evolution of NA in Victoria-lineage strains were faster compared with HA.Conclusion The genetic evolution rates of NA genes were faster than that of HA genes in the local circulated Victoria-lineage viruses during 2010 to 2012;The comprehensive analysis of HA and NA fragments were more reliable and sensitive on surveillance of genetic evolution of influenza B viruses.
