CAJ | 학술논문

目的调查胶东丘陵地区羊、牛、狗、猪、鸡等与人关系密切的家畜(禽)中新布尼亚病毒感染状况及流行规律.方法选择山东省胶东地区2010年有发热伴血小板减少综合征病例出现的蓬莱市和莱州市为调查点,于2011年4-11月,每月在每个调查点选择2个出现病例的乡镇,每个乡镇选择3个出现病例的村,采用随机数字表法选取羊、牛、狗、鸡、猪5种家畜(禽),每只采集静脉血5 ml,每种家畜(禽)40 ～60份,共采集3576份.应用双抗原夹心ELISA连续检测不同动物血清中新布尼亚病毒抗体.采用x2检验分析不同种类家畜(禽)间新布尼亚病毒感染率的差异,及蓬莱和莱州市不同种类家畜(禽)间感染率的差异.结果 3576份动物血清标本中,羊、牛、鸡、狗、猪的感染率分别为63％(636/1013)、53％(444/841)、46％(242/530)、29％(104/362)、1％(12/830),差异有统计学意义(x2=815.26,P＜0.05).蓬莱市羊、牛、鸡、及5种家畜(禽)总感染率分别为71％(400/563)、57％(232/409)、35％(93/266)、44％(796/1819),莱州市分别为53％(236/450)、49％(212/432)、56％ (149/264)、36％(642/1757),差异均有统计学意义(x2值分别为37.04、4.93、24.63、19.38,P值均＜0.05).羊的感染率存在夏季和秋季2个高峰,夏季6月份最高,为62％(68/110),秋季11月份最高,为86％(204/236);牛的感染率则存在春季和秋季两个高峰,春季4月份最高,为56％(53/94),秋季11月份最高,为73％ (116/159);鸡的感染率只有秋季1个高峰,9月份最高,为65％(55/85);狗和猪的感染率无季节高峰.结论胶东丘陵地区家畜(禽)中羊、牛、鸡、狗的感染率较高,高峰季节为春季、夏初及秋季.
목적 조사효동구릉지구양、우、구、저、계등여인관계밀절적가축(금)중신포니아병독감염상황급류행규률.방법 선택산동성효동지구2010년유발열반혈소판감소종합정병례출현적봉래시화래주시위조사점,우2011년4-11월,매월재매개조사점선택2개출현병례적향진,매개향진선택3개출현병례적촌,채용수궤수자표법선취양、우、구、계、저5충가축(금),매지채집정맥혈5 ml,매충가축(금)40 ～60빈,공채집3576빈.응용쌍항원협심ELISA련속검측불동동물혈청중신포니아병독항체.채용x2검험분석불동충류가축(금)간신포니아병독감염솔적차이,급봉래화래주시불동충류가축(금)간감염솔적차이.결과 3576빈동물혈청표본중,양、우、계、구、저적감염솔분별위63％(636/1013)、53％(444/841)、46％(242/530)、29％(104/362)、1％(12/830),차이유통계학의의(x2=815.26,P＜0.05).봉래시양、우、계、급5충가축(금)총감염솔분별위71％(400/563)、57％(232/409)、35％(93/266)、44％(796/1819),래주시분별위53％(236/450)、49％(212/432)、56％ (149/264)、36％(642/1757),차이균유통계학의의(x2치분별위37.04、4.93、24.63、19.38,P치균＜0.05).양적감염솔존재하계화추계2개고봉,하계6월빈최고,위62％(68/110),추계11월빈최고,위86％(204/236);우적감염솔칙존재춘계화추계량개고봉,춘계4월빈최고,위56％(53/94),추계11월빈최고,위73％ (116/159);계적감염솔지유추계1개고봉,9월빈최고,위65％(55/85);구화저적감염솔무계절고봉.결론 효동구릉지구가축(금)중양、우、계、구적감염솔교고,고봉계절위춘계、하초급추계.
Objective To understand the infection status and epidemic rule of new bunia virus in the livestock and poultry which are closely related with humans such as sheep,cattle,dogs,pigs and chicken in the hilly area of.Jiaodong peninsula in Shandong province.Methods Penglai and Laizhou in the hilly area of Jiaodong peninsula in Shandong province where severe fever with thrombocytopenia syndrome cases occurred in 2010 were selected as experimental sites.During April to November in 2011,serum specimens of the sheep,cattle,dogs,pigs and chicken with ticks in endemic area were randomly collected by random number table.5 ml venous blood was collected in each livestocks or poultries and there were total 3576 samples.New bunia virus antibody in different species of livestocks or poultries serum was continuously detected using double antigen sandwich enzyme-linked immunosorbeut assay,and the infection rates of new bunia virus between different species of livestocks or poultries and between Penglai and Laizhou were analyzed using chi-square test.Results Test results in 3576 samples of livestocks or poultries serum specimen showed that the infection rate was as high as 63％ (636/1013) in sheep,53％ (444/841)in cattle,46％ (242/530) in chicken,29％ (104/362) in the dogs,and 1％ (12/830) in pigs.There were significant differences of new bunia virus infection among different species (x2 =815.26,P ＜ 0.05).InPenglai,the infection rate was as high as 71％ (400/563) in sheep,57％ (232/409) in cattle,35％ (93/266) in chicken,44％ (796/1819) in total,while in Laizhou,the infection rate was 53 ％ (236/450) in sheep,49％ (212/432) in cattle,56％ (149/264) in chicken,36％ (642/1757) in total,their difference was statistically significant(x2 values were 37.04,4.93,24.63,19.38,all P values were ＜ 0.05).Infection rates of dogs and pigs showed no obvious fluctuation.However,there were two peaks of infection in sheep in summer and autumn,the infection rate was as high as 62％ (68/110) in June and 86％ (204/236) in November;There were two peaks of infection in cattle in spring and autumn,the infection rate was as high as 56％ (53/94) in April and 73％ (116/159) in November; there was only one peak of infection in chicken,the infection rate was as high as 65％ (55/85) in September.Conclusion The infection rate is higher in sheep,cattle,chickens and dogs in the hilly area of Jiaodong peninsula.The peak season is spring,summer and autumn.