中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2013年
12期
1142-1147
,共6页
邓超男%于燕妮%谢莹%赵丽娜
鄧超男%于燕妮%謝瑩%趙麗娜
산초남%우연니%사형%조려나
氟化物%睾丸%钙调神经磷酸酶%活化T细胞核因子
氟化物%睪汍%鈣調神經燐痠酶%活化T細胞覈因子
불화물%고환%개조신경린산매%활화T세포핵인자
Fluorides%Testis%CaN%NFATc1
目的 探讨钙调神经磷酸酶(CaN)和活化T细胞核因子1(NFATc1)在慢性氟中毒大鼠睾丸损伤机制中的意义.方法 18只6周龄清洁级雄性SD大鼠按体重用随机数字表法分为3组(每组6只),对照组饮用自来水(含氟量＜1 mg/L),低氟组和高氟组分别饮用含5、50 mg/L氟化钠的自来水.饲养8个月后,观察氟斑牙发生情况,氟离子选择电极法检测尿氟含量情况;称量各组大鼠体重及睾丸质量;光镜观察各组大鼠睾丸组织形态学变化;免疫组化和原位杂交检测睾丸组织CaN和NFATc1的蛋白及mRNA表达情况.结果 大鼠氟斑牙检出只数为对照组0只,低氟组4只(4/6),高氟组5只(5/6),差异有统计学意义(x2=10.60,P＜0.05).在对照、低氟组、高氟组尿氟含量依次升高,分别为(1.26 ±0.17)、(2.06±0.64)、(7.69±1.96) mg/L(F=36.57,P＜0.05);体重分别为(629.00±16.00)、(585.17±17.27)、(560.50±16.07)g,差异有统计学意义(F=26.67,P＜0.05);睾丸质量分别为(2.58 ±0.17)、(2.43±0.31)、(2.35 ±0.38)g,差异无统计学意义(F=0.91,P＞0.05).与对照组比较,各染氟组大鼠各级生精小管结构出现不同程度破坏,高氟组破坏程度最大.对照组、低氟组、高氟组睾丸组织中CaN蛋白表达灰度值依次增加,分别为59.10±5.62、77.93±4.16、101.69 ±6.31 (F=74.18,P＜0.05);NFATc1蛋白表达灰度值分别为76.11 ±4.41、93.42±3.85、120.42±9.31,随染氟增加而逐渐增加(F =92.4,P＜0.05).CaN的mRNA表达量分别为58.76±7.70、82.01±6.88、99.47±8.33(F=42.65,P＜0.05),NFATc1分别为59.39±4.74、90.02±5.37、121.15±7.69(F=155.47,P＜0.05).CaN和NFATc1蛋白及mRNA表达水平呈正相关(r分别为0.899、0.908).结论 CaN依赖的信号通路表达变化可能参与到慢性氟中毒大鼠睾丸组织的损伤机制中.
目的 探討鈣調神經燐痠酶(CaN)和活化T細胞覈因子1(NFATc1)在慢性氟中毒大鼠睪汍損傷機製中的意義.方法 18隻6週齡清潔級雄性SD大鼠按體重用隨機數字錶法分為3組(每組6隻),對照組飲用自來水(含氟量＜1 mg/L),低氟組和高氟組分彆飲用含5、50 mg/L氟化鈉的自來水.飼養8箇月後,觀察氟斑牙髮生情況,氟離子選擇電極法檢測尿氟含量情況;稱量各組大鼠體重及睪汍質量;光鏡觀察各組大鼠睪汍組織形態學變化;免疫組化和原位雜交檢測睪汍組織CaN和NFATc1的蛋白及mRNA錶達情況.結果 大鼠氟斑牙檢齣隻數為對照組0隻,低氟組4隻(4/6),高氟組5隻(5/6),差異有統計學意義(x2=10.60,P＜0.05).在對照、低氟組、高氟組尿氟含量依次升高,分彆為(1.26 ±0.17)、(2.06±0.64)、(7.69±1.96) mg/L(F=36.57,P＜0.05);體重分彆為(629.00±16.00)、(585.17±17.27)、(560.50±16.07)g,差異有統計學意義(F=26.67,P＜0.05);睪汍質量分彆為(2.58 ±0.17)、(2.43±0.31)、(2.35 ±0.38)g,差異無統計學意義(F=0.91,P＞0.05).與對照組比較,各染氟組大鼠各級生精小管結構齣現不同程度破壞,高氟組破壞程度最大.對照組、低氟組、高氟組睪汍組織中CaN蛋白錶達灰度值依次增加,分彆為59.10±5.62、77.93±4.16、101.69 ±6.31 (F=74.18,P＜0.05);NFATc1蛋白錶達灰度值分彆為76.11 ±4.41、93.42±3.85、120.42±9.31,隨染氟增加而逐漸增加(F =92.4,P＜0.05).CaN的mRNA錶達量分彆為58.76±7.70、82.01±6.88、99.47±8.33(F=42.65,P＜0.05),NFATc1分彆為59.39±4.74、90.02±5.37、121.15±7.69(F=155.47,P＜0.05).CaN和NFATc1蛋白及mRNA錶達水平呈正相關(r分彆為0.899、0.908).結論 CaN依賴的信號通路錶達變化可能參與到慢性氟中毒大鼠睪汍組織的損傷機製中.
목적 탐토개조신경린산매(CaN)화활화T세포핵인자1(NFATc1)재만성불중독대서고환손상궤제중적의의.방법 18지6주령청길급웅성SD대서안체중용수궤수자표법분위3조(매조6지),대조조음용자래수(함불량＜1 mg/L),저불조화고불조분별음용함5、50 mg/L불화납적자래수.사양8개월후,관찰불반아발생정황,불리자선택전겁법검측뇨불함량정황;칭량각조대서체중급고환질량;광경관찰각조대서고환조직형태학변화;면역조화화원위잡교검측고환조직CaN화NFATc1적단백급mRNA표체정황.결과 대서불반아검출지수위대조조0지,저불조4지(4/6),고불조5지(5/6),차이유통계학의의(x2=10.60,P＜0.05).재대조、저불조、고불조뇨불함량의차승고,분별위(1.26 ±0.17)、(2.06±0.64)、(7.69±1.96) mg/L(F=36.57,P＜0.05);체중분별위(629.00±16.00)、(585.17±17.27)、(560.50±16.07)g,차이유통계학의의(F=26.67,P＜0.05);고환질량분별위(2.58 ±0.17)、(2.43±0.31)、(2.35 ±0.38)g,차이무통계학의의(F=0.91,P＞0.05).여대조조비교,각염불조대서각급생정소관결구출현불동정도파배,고불조파배정도최대.대조조、저불조、고불조고환조직중CaN단백표체회도치의차증가,분별위59.10±5.62、77.93±4.16、101.69 ±6.31 (F=74.18,P＜0.05);NFATc1단백표체회도치분별위76.11 ±4.41、93.42±3.85、120.42±9.31,수염불증가이축점증가(F =92.4,P＜0.05).CaN적mRNA표체량분별위58.76±7.70、82.01±6.88、99.47±8.33(F=42.65,P＜0.05),NFATc1분별위59.39±4.74、90.02±5.37、121.15±7.69(F=155.47,P＜0.05).CaN화NFATc1단백급mRNA표체수평정정상관(r분별위0.899、0.908).결론 CaN의뢰적신호통로표체변화가능삼여도만성불중독대서고환조직적손상궤제중.
Objective To discuss the significance of calcineurin (CaN) and nuclear factor of active T cells 1 (NFATc1) in the damage mechanism of the testis of rats with chronic fluorosis.Methods Eighteen clear class SD male rats,aging 6 week-old,were randomly divided into 3 groups,6 rats in each.The rats of control group were fed with tap water (NaF ＜ 1 mg/L) and the experimental rats were exposed to NaF (lower group:5 mg/L,higher group:50 mg/L) to established the chronic fluorosis model.After 8 months,we observed the occurance of dental fluorosis among rats in different groups,and the contents of urine fluoride were detected by fluorine ion selective electrode method.The body of the rats were weighted as well as their testis.The testis tissues were stained with hematoxylin-eosin and observed under light microscope to find the morphological changes.The expression of CaN and NFATc1's protein and mRNA in testis were detected by Immunocytochemistry (IHC) and In-situ hybridization (ISH).Results The number of rats which was found dental fluorosis were separately 0,4 and 5 in control group,low dose group and high dose group (x2 =10.60,P ＜ 0.05).The contents of urine fluoride were gradually increased in control group,low group and high group,which were (1.26 ±0.17),(2.06 ±0.64) and (7.69 ± 1.96) mg/L,respectively (F =36.57,P ＜ 0.05).The body weight were significantly different in all three groups (629.00 ± 16.00),(585.17 ± 17.27),(560.50 ± 16.07) g,F =26.67,P ＜ 0.05) and the testis weight were without statistical difference ((2.58 ± 0.17),(2.43 ± 0.31),(2.35 ± 0.38) g,F =0.91,P ＞0.05).Compared with the control group,the testicular structures were damaged in the experimental groups and especially significant in high dose group.The expression of CaN (59.10 ± 5.62,77.93 ± 4.16,101.69 ± 6.31,F =74.18,P ＜ 0.05) and NFATc1's (76.11 ± 4.41,93.42 ± 3.85,120.42 ± 9.31,F =92.4,P ＜ 0.05) protein in testis tissues were increased by the fluorine concentration.The mRNA expression of CaN and NFATc1 were separately (CaN:58.76 ± 7.70,82.01 ± 6.88,99.47 ± 8.33,F =42.65,P ＜0.05 andNFATc1:59.39 ±4.74,90.02 ±5.37,121.15 ±7.69,F=155.47,P＜0.05).There were positive correlation between the expression of CaN and NFATc1's protein and mRNA expression (r =0.899,r =0.908).Conclusion The changes in the signaling pathway of expression of CaN may be involved in the injury mechanism of testis tissues of rats with chronic fluorosis.
