CAJ | 학술논문

目的研究孕妇尿样中邻苯二甲酸酯(PAEs)代谢产物与脐带血中抑制素B(INHB)、胰岛素样因子3(INSL3)水平的关联性.方法将2011年6月至2012年9月在天津某三级甲等妇产科医院进行产前检查的孕妇作为研究对象,采集孕期晨尿中段尿,随访其妊娠过程直至分娩,抽取脐带血10 ml,追踪到符合条件的孕妇-新生儿共69对.应用固相萃取-三重四极杆高效液相色谱串联质谱技术检测孕期尿样中PAEs的4种代谢产物[邻苯二甲酸单甲酯(MMP)、邻苯二甲酸单乙基己基酯(MEHP)、邻苯二甲酸单乙酯(MEP)、邻苯二甲酸单丁酯(MBP)]水平.应用ELISA测定脐血样品中INHB、INSL3水平,应用多元线性回归模型进行关联性分析.结果孕妇尿中MMP、MEHP、MEP和MBP中位数分别为49.74、24.96、19.52和17.73 μg/L；脐带血中INHB和INSL3中位数分别为89.09和106.21 ng/L.MMP(β '=-0.252)、MEP(β'=-0.363)和∑PAEs(β'=-0.346)的改变对INHB水平的影响具有统计学意义,且呈负相关；∑PAEs(β'=-0.313)的改变对INSL3水平的影响具有统计学意义,且呈负相关.结论孕期暴露PAEs代谢产物水平与脐血中INHB和INSL3水平具有关联性.
목적 연구잉부뇨양중린분이갑산지(PAEs)대사산물여제대혈중억제소B(INHB)、이도소양인자3(INSL3)수평적관련성.방법 장2011년6월지2012년9월재천진모삼급갑등부산과의원진행산전검사적잉부작위연구대상,채집잉기신뇨중단뇨,수방기임신과정직지분면,추취제대혈10 ml,추종도부합조건적잉부-신생인공69대.응용고상췌취-삼중사겁간고효액상색보천련질보기술검측잉기뇨양중PAEs적4충대사산물[린분이갑산단갑지(MMP)、린분이갑산단을기기기지(MEHP)、린분이갑산단을지(MEP)、린분이갑산단정지(MBP)]수평.응용ELISA측정제혈양품중INHB、INSL3수평,응용다원선성회귀모형진행관련성분석.결과 잉부뇨중MMP、MEHP、MEP화MBP중위수분별위49.74、24.96、19.52화17.73 μg/L；제대혈중INHB화INSL3중위수분별위89.09화106.21 ng/L.MMP(β '=-0.252)、MEP(β'=-0.363)화∑PAEs(β'=-0.346)적개변대INHB수평적영향구유통계학의의,차정부상관；∑PAEs(β'=-0.313)적개변대INSL3수평적영향구유통계학의의,차정부상관.결론 잉기폭로PAEs대사산물수평여제혈중INHB화INSL3수평구유관련성.
Objective The purposes of our study were to investigate the association between maternal urinary phthalate metabolites and the levels of inhibin B (INHB) and insulin-like factor 3 (INSL3)in the cord blood in a Chinese pregnant population.Methods Maternal urine samples in the third trimester of pregnancy of 69 participants were collected and stored,and the samples of cord blood(10 ml) were collected at delivery between June 2011 and September 2012 in a comprehensive hospital of gynecology and obstetrics in Tianjin,China.Four phthalate metabolites,monomethyl phthalate (MMP),monoethyl phthalate (MEP),monobutyl phthalate (M BP),and mono-2-ethylhexyl phthalate (MEHP) were measured in the urine samples using liquid chromatography-tandem mass spectrometry.The levels of INHB,INSL3 in the cord blood were tested by ELISA.Associations of phthalate exposure with INHB and INSL3 levels were determined by spearman correlation and multiple regression model analysis.Results The median concentrations of observed metabolites in descending order were 49.74 μg/L for MMP,24.96 μg/L for MEHP,19.52 μg/L for MEP and 17.73 μg/L for MBP.The median concentrations of INHB and INSL3 were 89.09 and 106.21 ng/L.Significant negative associations between INHB and MMP (β' =-0.252),MEP (β' =-0.363) or the sum value (ΣPAEs) (β' =-0.346) were found by the multiple regression model analysis.For INSL3,only the sum value (β' =-0.313) was inversely significantly associated with the levels of INSL3 in the cord blood.Conclusions Maternal urinary phthalate metabolites were associated with INHB and INSL3 in the cord blood in a Chinese population.