CAJ | 학술논문

目的检测广州市2010-2012年手足口病(HFMD)病例粪便样本中的肠道病毒,并对其中的柯萨奇病毒A4型(CoxA4)和柯萨奇病毒A10型(CoxA10) VP1基因核苷酸序列进行进化分析.方法收集2010-2012年广州市HFMD疑似病例粪便样本5 484份,采用RT-PCR进行肠道病毒71型(EV71)、柯萨奇病毒A16型(CoxA16)、CoxA4、CoxA10和其他肠道病毒阳性检测,阳性样本共4 111份.对其中的CoxA4及CoxA10阳性样本分别进行巢式RT-PCR,扩增肠道病毒VP1基因片段,并对VP1基因片段进行测序,利用生物信息学分析序列,构建系统进化树,进行进化分析.结果在4 111份肠道病毒阳性样本中,EV71、CoxA16、CoxA10、CoxA4的阳性率分别为35.1％(1443/4111)、30.7％(1261/4 111)、2.0％(82/4 111)、0.8％(31/4 111),不同肠道病毒阳性率差异有统计学意义(x2=148.34,P＜0.05).CoxA4样本以3岁组阳性率最高,为1.3％ (7/534),CoxA10样本以0岁组阳性率最高,为3.7％(34/914);CoxA4样本阳性率4月份达到高峰,为2.6％(12/460),CoxA10样本阳性率8月份达到高峰,为4.3％(12/278).VP1基因核苷酸遗传进化分析显示,CoxA4阳性样本VP1基因核苷酸序列可分为A、B2个亚型,Cox A10阳性样本VP1基因核苷酸序列可分为A、B、C3个亚型,广州市2010-2012年CoxA4和CoxA10阳性样本VP1基因核苷酸序列均属于A亚型.结论广州市2010-2012年HFMD病例粪便样本中CoxA4和CoxA10阳性样本VP1基因核苷酸序列均属于A亚型.
목적 검측엄주시2010-2012년수족구병(HFMD)병례분편양본중적장도병독,병대기중적가살기병독A4형(CoxA4)화가살기병독A10형(CoxA10) VP1기인핵감산서렬진행진화분석.방법 수집2010-2012년엄주시HFMD의사병례분편양본5 484빈,채용RT-PCR진행장도병독71형(EV71)、가살기병독A16형(CoxA16)、CoxA4、CoxA10화기타장도병독양성검측,양성양본공4 111빈.대기중적CoxA4급CoxA10양성양본분별진행소식RT-PCR,확증장도병독VP1기인편단,병대VP1기인편단진행측서,이용생물신식학분석서렬,구건계통진화수,진행진화분석.결과 재4 111빈장도병독양성양본중,EV71、CoxA16、CoxA10、CoxA4적양성솔분별위35.1％(1443/4111)、30.7％(1261/4 111)、2.0％(82/4 111)、0.8％(31/4 111),불동장도병독양성솔차이유통계학의의(x2=148.34,P＜0.05).CoxA4양본이3세조양성솔최고,위1.3％ (7/534),CoxA10양본이0세조양성솔최고,위3.7％(34/914);CoxA4양본양성솔4월빈체도고봉,위2.6％(12/460),CoxA10양본양성솔8월빈체도고봉,위4.3％(12/278).VP1기인핵감산유전진화분석현시,CoxA4양성양본VP1기인핵감산서렬가분위A、B2개아형,Cox A10양성양본VP1기인핵감산서렬가분위A、B、C3개아형,엄주시2010-2012년CoxA4화CoxA10양성양본VP1기인핵감산서렬균속우A아형.결론 엄주시2010-2012년HFMD병례분편양본중CoxA4화CoxA10양성양본VP1기인핵감산서렬균속우A아형.
Objective To identify the enterovirus from stool samples of patients with hand,foot and mouth disease(HFMD) in Guangzhou from 2010 to 2012 and to perform phylogenetic analysis of the VP1 gene sequences of coxsackievirus A4 and coxsackievirus A10.Methods A total of 5 484 samples of suspected cases of HFMD which Guangzhou Center for Disease Control received from 2010 to 2012 were collected.Virus RNA was tested by nested RT-PCR method as human enterovirus 71,coxsackievirus A16,coxsackievirus A4,coxsackievirus A10 and other enteroviruses positive,and 4 111 samples were positive.Phylogenetic tree was constructed by partial VP1 gene sequences of coxsackievirus A4 and coxsackievirus A10 to perform phylogenetic analysis.Results In 4 111 enterovirus-positive samples,the positive rate of EV71,CoxA16,CoxA10 and CoxA4 was 35.1％ (1 443/4 111),30.7％ (1 261/4 111),2.0％ (82/4 111),0.8％ (31/4 111) respectively.Different enterovirus-positive rate was statistically significant(x2 =148.34,P ＜ 0.05).Incidences of coxsackievirus A4 positive was highest in 3-year old children as 1.3％ (7/534),and that of coxsackievirus A10 positive was highest in 0-year old children as 3.7％ (34/914).The highest positive rate of diagnosed coxsackievirus A4 positive cases was admitted in April(2.6％,12/460),and the highest positive rate of diagnosed coxsackievirus A10 positive cases was admitted in August 4.3％ (12/278).Phylogenetic analysis indicated that all the CoxA4 stains were divided into subtype A and subtype B,and the CoxA10 stains were divided into subtypes A,subtype B and subtype C.The VP1 gene nucleotide sequences of CoxA4 and CoxA10 this study measured both belonged to subtype A.Conclusions The VP1 gene nucleotide sequences of CoxA4 and CoxA10 in Guangzhou from 2010 to 2012 both belonged to subtype A.