中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2014年
6期
456-460
,共5页
袁玉起%吕慧%周海健%崔志刚%孙娜%关冰%邵坤%毕振旺%阚飙
袁玉起%呂慧%週海健%崔誌剛%孫娜%關冰%邵坤%畢振旺%闞飆
원옥기%려혜%주해건%최지강%손나%관빙%소곤%필진왕%감표
弧菌,霍乱%电泳,凝胶,脉冲场%毒力%耐药
弧菌,霍亂%電泳,凝膠,脈遲場%毒力%耐藥
호균,곽란%전영,응효,맥충장%독력%내약
Vibrio cholerae%Electrophoresis,gel,pulsed-field%Virulence%Antibiotic resistance
目的 调查山东省O139群霍乱弧菌分子流行病学特征及耐药谱.方法 对山东省自1997年O139群霍乱疫情出现以来分离的13株O139群霍乱弧菌(9株为临床分离株,4株为环境株)进行生化鉴定及血清型复核,同时进行PFGE分子分型和毒力基因及耐药相关基因检测,使用微量肉汤稀释法检测其耐药性.结果 山东省13株O139群霍乱弧菌聚类为7个PFGE型别,其中2013年济宁的1株临床株和2株环境株聚为KZGN11O139.CN0077,2005年济南的1株临床株、2005年济宁的1株临床株和2009年菏泽的1临床株聚为KZGN11O139.CN0002,其余均各自为不同的型别.2株烟台临床株缺失ctxAB和tcpI基因,PFGE条带也较为特殊,其他11株霍乱弧菌均含有hlyA、ctxAB、toxR、tcpI、rtx和tcpA6种毒力基因.13株霍乱弧菌均含有耐药相关基因intI1、intI4及sxt.在12种抗生素中,卡那霉素、复方磺胺甲恶唑、氨苄西林及庆大霉素的耐药率分别为11/13、9/13、7/13和7/13.结论 山东省O139群霍乱弧菌PEGE分型提示菌株间可能存在流行病学关联,多数菌株产毒且耐药.
目的 調查山東省O139群霍亂弧菌分子流行病學特徵及耐藥譜.方法 對山東省自1997年O139群霍亂疫情齣現以來分離的13株O139群霍亂弧菌(9株為臨床分離株,4株為環境株)進行生化鑒定及血清型複覈,同時進行PFGE分子分型和毒力基因及耐藥相關基因檢測,使用微量肉湯稀釋法檢測其耐藥性.結果 山東省13株O139群霍亂弧菌聚類為7箇PFGE型彆,其中2013年濟寧的1株臨床株和2株環境株聚為KZGN11O139.CN0077,2005年濟南的1株臨床株、2005年濟寧的1株臨床株和2009年菏澤的1臨床株聚為KZGN11O139.CN0002,其餘均各自為不同的型彆.2株煙檯臨床株缺失ctxAB和tcpI基因,PFGE條帶也較為特殊,其他11株霍亂弧菌均含有hlyA、ctxAB、toxR、tcpI、rtx和tcpA6種毒力基因.13株霍亂弧菌均含有耐藥相關基因intI1、intI4及sxt.在12種抗生素中,卡那黴素、複方磺胺甲噁唑、氨芐西林及慶大黴素的耐藥率分彆為11/13、9/13、7/13和7/13.結論 山東省O139群霍亂弧菌PEGE分型提示菌株間可能存在流行病學關聯,多數菌株產毒且耐藥.
목적 조사산동성O139군곽란호균분자류행병학특정급내약보.방법 대산동성자1997년O139군곽란역정출현이래분리적13주O139군곽란호균(9주위림상분리주,4주위배경주)진행생화감정급혈청형복핵,동시진행PFGE분자분형화독력기인급내약상관기인검측,사용미량육탕희석법검측기내약성.결과 산동성13주O139군곽란호균취류위7개PFGE형별,기중2013년제저적1주림상주화2주배경주취위KZGN11O139.CN0077,2005년제남적1주림상주、2005년제저적1주림상주화2009년하택적1림상주취위KZGN11O139.CN0002,기여균각자위불동적형별.2주연태림상주결실ctxAB화tcpI기인,PFGE조대야교위특수,기타11주곽란호균균함유hlyA、ctxAB、toxR、tcpI、rtx화tcpA6충독력기인.13주곽란호균균함유내약상관기인intI1、intI4급sxt.재12충항생소중,잡나매소、복방광알갑악서、안변서림급경대매소적내약솔분별위11/13、9/13、7/13화7/13.결론 산동성O139군곽란호균PEGE분형제시균주간가능존재류행병학관련,다수균주산독차내약.
Objective To investigate the molecular epidemiological characteristics and antibiotic resistance profiles of Vibrio cholerae O139 in Shandong province.Methods A total of 13 strains of V.cholerae O139 (9 clinical strains and 4 environmental strains) isolated from cholera epidemics in Shandong province since 1997 were recovered and confirmed with serum agglutination and biochemical reaction.Pulsed-field gel electrophoresis (PFGE) was carried out for molecular subtyping.Virulence genes and drug resistance related genes were detected by PCR.Antibiotic susceptibility tests were performed using micro-broth dilution method.Results Thirteen strains of V.cholerae O139 were differentiated into seven pulsetypes.One clinical strain and two environmental strains isolated from Jining in 2013 were clustered into the pulsetype namely KZGN11 O139.CN0077,and an identical PFGE pattern of KZGN11O139.CN0002 was found among three clinical strains from Jinan in 2005,Jining in 2005 and Heze in 2009.Other pulsotypes were unique in China and found only in Shandong province.Because of deletion of ctxAB and tcpI,the PFGE patterns of two strains isolated from Yantai in 2000 and 2004 were different from other 11 strains which harbored ctxAB,tcpA,tcpI,rtxA,hlyA and toxR.All strains contained one or more drug resistance related genes such as intI 1,intI 4 and sxt,and were resistant to two kinds of antibiotics at least.Among the 12 kinds of antibiotics,the resistant ratioes to kamamycin,trimethoprim-sulfamethoxazole,ampicillin and gentamicin were 11/13,9/13,7/13 and 7/13,respectively.Conclusion Molecular subtyping indicates possible epidemiological links among V.cholerae O139 in Shandong province,and almost all strains were toxigenic and drug resistant.
