中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2014年
6期
461-465
,共5页
梁未丽%赵璇%张力%崔志刚%李杰%阚飙
樑未麗%趙璇%張力%崔誌剛%李傑%闞飆
량미려%조선%장력%최지강%리걸%감표
弧菌,霍乱,O1%基因型%遗传变异
弧菌,霍亂,O1%基因型%遺傳變異
호균,곽란,O1%기인형%유전변이
Vibrio cholerae O1%Genotype%Genetic variation
目的 分析1961-2010年中国不同地区分离的O1群El Tor型霍乱弧菌产毒株的ctxB变异特征和rstR基因类型.方法 选取中国1961-2010年间分离的O1群霍乱弧菌菌株作为研究对象,共385株.应用PCR方法扩增ctxB基因并进行序列测定和分析;使用rstR型特异性引物扩增rstR基因,确定其基因型别.结果 ctxB基因序列分析表明,385株被检菌株中52.5％(202/385)携带ctxBET,47.5％(183/385)携带ctxBc1ass,即第39位的酪氨酸(Y)变为组氨酸(H),68位的异亮氨酸(Ⅰ)变为苏氨酸(T),呈现古典生物型菌株(O395)的序列特征.1961-1992年以携带ctxBET的菌株占绝对优势,比例高达98.4％(182/185);1993年后,携带ctxBclass的菌株增加,其中1993-2005年间携带ctxBc1ass菌株的比例达到97.2％ (174/179);自2006年开始,重新出现了ctxBET为主导或二者共存的局面.rstR基因的检测表明约62.9％(242/385)的菌株携带rstRET,6.8％ (26/385)的菌株携带rstRclass,其余菌株中存在不同的rstR组合形式,其中,以rstRET+ rstRc1as的组合最多,占75.7％(75/99).与ctxB相似,不同型别rstR的分布呈现时间特异性,1961-1992年期间以携带rstRET的菌株占主导(87％,161/185),1993年以后出现多态性变异,携带其他类型rstR如rstRclass,rstRenv及不同rstR组合的菌株明显增多,与rstRET共同存在.96％(25/26)、84％(63/75)和18/18的分别携带rstRclass、rstRET+ rstRclass组合和rstRET+ rstRc1ass+ rstRenv组合的菌株均分离于1993年之后.结论 中国霍乱弧菌菌株中ctxB和rstR的型别分布有明显的时间特异性,rstR存在多种组合形式,反映了菌株多样性的遗传和进化特征.
目的 分析1961-2010年中國不同地區分離的O1群El Tor型霍亂弧菌產毒株的ctxB變異特徵和rstR基因類型.方法 選取中國1961-2010年間分離的O1群霍亂弧菌菌株作為研究對象,共385株.應用PCR方法擴增ctxB基因併進行序列測定和分析;使用rstR型特異性引物擴增rstR基因,確定其基因型彆.結果 ctxB基因序列分析錶明,385株被檢菌株中52.5％(202/385)攜帶ctxBET,47.5％(183/385)攜帶ctxBc1ass,即第39位的酪氨痠(Y)變為組氨痠(H),68位的異亮氨痠(Ⅰ)變為囌氨痠(T),呈現古典生物型菌株(O395)的序列特徵.1961-1992年以攜帶ctxBET的菌株佔絕對優勢,比例高達98.4％(182/185);1993年後,攜帶ctxBclass的菌株增加,其中1993-2005年間攜帶ctxBc1ass菌株的比例達到97.2％ (174/179);自2006年開始,重新齣現瞭ctxBET為主導或二者共存的跼麵.rstR基因的檢測錶明約62.9％(242/385)的菌株攜帶rstRET,6.8％ (26/385)的菌株攜帶rstRclass,其餘菌株中存在不同的rstR組閤形式,其中,以rstRET+ rstRc1as的組閤最多,佔75.7％(75/99).與ctxB相似,不同型彆rstR的分佈呈現時間特異性,1961-1992年期間以攜帶rstRET的菌株佔主導(87％,161/185),1993年以後齣現多態性變異,攜帶其他類型rstR如rstRclass,rstRenv及不同rstR組閤的菌株明顯增多,與rstRET共同存在.96％(25/26)、84％(63/75)和18/18的分彆攜帶rstRclass、rstRET+ rstRclass組閤和rstRET+ rstRc1ass+ rstRenv組閤的菌株均分離于1993年之後.結論 中國霍亂弧菌菌株中ctxB和rstR的型彆分佈有明顯的時間特異性,rstR存在多種組閤形式,反映瞭菌株多樣性的遺傳和進化特徵.
목적 분석1961-2010년중국불동지구분리적O1군El Tor형곽란호균산독주적ctxB변이특정화rstR기인류형.방법 선취중국1961-2010년간분리적O1군곽란호균균주작위연구대상,공385주.응용PCR방법확증ctxB기인병진행서렬측정화분석;사용rstR형특이성인물확증rstR기인,학정기기인형별.결과 ctxB기인서렬분석표명,385주피검균주중52.5％(202/385)휴대ctxBET,47.5％(183/385)휴대ctxBc1ass,즉제39위적락안산(Y)변위조안산(H),68위적이량안산(Ⅰ)변위소안산(T),정현고전생물형균주(O395)적서렬특정.1961-1992년이휴대ctxBET적균주점절대우세,비례고체98.4％(182/185);1993년후,휴대ctxBclass적균주증가,기중1993-2005년간휴대ctxBc1ass균주적비례체도97.2％ (174/179);자2006년개시,중신출현료ctxBET위주도혹이자공존적국면.rstR기인적검측표명약62.9％(242/385)적균주휴대rstRET,6.8％ (26/385)적균주휴대rstRclass,기여균주중존재불동적rstR조합형식,기중,이rstRET+ rstRc1as적조합최다,점75.7％(75/99).여ctxB상사,불동형별rstR적분포정현시간특이성,1961-1992년기간이휴대rstRET적균주점주도(87％,161/185),1993년이후출현다태성변이,휴대기타류형rstR여rstRclass,rstRenv급불동rstR조합적균주명현증다,여rstRET공동존재.96％(25/26)、84％(63/75)화18/18적분별휴대rstRclass、rstRET+ rstRclass조합화rstRET+ rstRc1ass+ rstRenv조합적균주균분리우1993년지후.결론 중국곽란호균균주중ctxB화rstR적형별분포유명현적시간특이성,rstR존재다충조합형식,반영료균주다양성적유전화진화특정.
Objective To understand the ctxB and rstR variations of toxigenic Vibrio cholerae (V.cholerae) O1 El Tor strains isolated from different provinces in China from 1961 to 2010.Methods All 385 toxigenic V.cholerae O1 El Tor strains were selected,which were isolated in China between year 1961 and 2010.ctxB gene was amplified by PCR method and sequenced for further analysis.rstR was detected with PCR by using the genotype specific primers.Results ctxB sequence analysis revealed that 52.5％ (202/385)isolates carried ctxBET and 47.5％ (183/385)carried ctxBclass,namely Y39 to H and I68 to T substitutions which were specific to the classical biotype CT-B sequence.From 1961 to 1992,strains carrying ctxBET were predominant and the proportion was as high as 98.4％ (182/185).After 1993,strains carrying ctxBclass were sharply increased.Especially during year 1993 to year 2005,97.2％ (174/179) of the isolated strains carried ctxBclass.Since 2006,resurgence of dominant strains carrying ctxBET or co-existing of strains with ctxBET or ctxBclass was noticed.rstR genotype detection showed that 62.9％ (242/385) of the tested strains carried the rstRET,while 6.8％ (26/385) with rstRclass,and the remainings contained at least two types of rstR in different combination forms,among which rstRET + rstRclass combination were the most,accounting for 75.7％ (75/99).Similar to the ctxB,the distribution of rstR genotypes showed time specificity.From 1961 to 1992,strains carrying rstRET predominated (87.0％,161/185).After 1993,the diversity of rstR genotypes was observed accompanying by a sharp increase of strains containing other rstR genotype,such as rstRclass,rstRenv and different combinations.There were separately 96％ (25/26),84％ (63/75) and 18/18 strains containing rstRclass,rstRET + rstRclass and rstRET + rstRclass + rstRenv isolated after 1993.Conclusion The distribution of different genotypes of ctxB and rstR showed obvious time-specificity,and there were various combining forms of rstR,reflecting the diversity of the genetic and evolutionary characteristics of Chinese V.cholerae isolates.
