中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2014年
6期
517-520
,共4页
谢佳新%高秋菊%杨丹%刘天鹏%曹广文
謝佳新%高鞦菊%楊丹%劉天鵬%曹廣文
사가신%고추국%양단%류천붕%조엄문
癌,肝细胞%STAT3转录因子%病例对照研究%多态现象,遗传
癌,肝細胞%STAT3轉錄因子%病例對照研究%多態現象,遺傳
암,간세포%STAT3전록인자%병례대조연구%다태현상,유전
Carcinoma,hepatocellular%STAT3 transcription factor%Case-control study%Polymorphism,genetic
目的 探讨信号传导与转录激活因子3(signal transducer and activators of transcription 3,STAT3)基因启动子区-1096G/C多态性与乙肝表面抗原(HBsAg)阳性肝细胞癌(hepatocellular carcinoma,HCC)易感性之间的关系.方法 采用病例-对照研究方法,应用荧光探针实时定量PCR法对2009至2012年在上海某医院就诊的632例HCC患者和723名非HCC乙肝病毒(HBV)感染者的STAT3基因启动子区-1096G/C基因多态性进行检测,使用单因素分析方法确定该基因多态性OR(95％ CI)值.结果 对照组、病例组携带STAT3-1096C(GC+ CC)基因型者分别占61.8％ (447/723)、60.6％(383/632),不同基因型之间HCC发病风险差异无统计学意义(OR =0.95,95％ CI:0.76～1.18).以性别进行分层分析,在男性人群中,对照组、病例组携带STAT3-1096 C(GC+ CC)基因型者分别占62.2％(314/505)、61.8％(331/536),在女性人群中,分别为61.0％(133/218)、54.17％ (52/96),在男性和女性人群中,不同基因型HCC发病风险差异均没有统计学意义(OR=0.98,95％ CI:0.77～1.26;OR =0.76,95％CI:0.47 ～ 1.26).以HBV基因型进行分层分析,在HBV B型感染者中,对照组、病例组携带STAT3-1096C(GC+ CC)基因型者分别占61.5％(110/179)、53.1％(34/64),在HBV C型感染者中,对照组、病例组携带STAT3-1096C(GC+CC)基因型者分别占62.9％(276/439)、60.3％(226/375),在HBV B或C型感染者中,不同基因型患者HCC发病风险差异没有统计学意义(OR =0.71,95％ CI:0.40～1.26;OR =0.90,95％ CI:0.68～ 1.19).结论 STAT3-1096G/C基因多态性与HBV阳性HCC的易感性没有相关性.
目的 探討信號傳導與轉錄激活因子3(signal transducer and activators of transcription 3,STAT3)基因啟動子區-1096G/C多態性與乙肝錶麵抗原(HBsAg)暘性肝細胞癌(hepatocellular carcinoma,HCC)易感性之間的關繫.方法 採用病例-對照研究方法,應用熒光探針實時定量PCR法對2009至2012年在上海某醫院就診的632例HCC患者和723名非HCC乙肝病毒(HBV)感染者的STAT3基因啟動子區-1096G/C基因多態性進行檢測,使用單因素分析方法確定該基因多態性OR(95％ CI)值.結果 對照組、病例組攜帶STAT3-1096C(GC+ CC)基因型者分彆佔61.8％ (447/723)、60.6％(383/632),不同基因型之間HCC髮病風險差異無統計學意義(OR =0.95,95％ CI:0.76～1.18).以性彆進行分層分析,在男性人群中,對照組、病例組攜帶STAT3-1096 C(GC+ CC)基因型者分彆佔62.2％(314/505)、61.8％(331/536),在女性人群中,分彆為61.0％(133/218)、54.17％ (52/96),在男性和女性人群中,不同基因型HCC髮病風險差異均沒有統計學意義(OR=0.98,95％ CI:0.77～1.26;OR =0.76,95％CI:0.47 ～ 1.26).以HBV基因型進行分層分析,在HBV B型感染者中,對照組、病例組攜帶STAT3-1096C(GC+ CC)基因型者分彆佔61.5％(110/179)、53.1％(34/64),在HBV C型感染者中,對照組、病例組攜帶STAT3-1096C(GC+CC)基因型者分彆佔62.9％(276/439)、60.3％(226/375),在HBV B或C型感染者中,不同基因型患者HCC髮病風險差異沒有統計學意義(OR =0.71,95％ CI:0.40～1.26;OR =0.90,95％ CI:0.68～ 1.19).結論 STAT3-1096G/C基因多態性與HBV暘性HCC的易感性沒有相關性.
목적 탐토신호전도여전록격활인자3(signal transducer and activators of transcription 3,STAT3)기인계동자구-1096G/C다태성여을간표면항원(HBsAg)양성간세포암(hepatocellular carcinoma,HCC)역감성지간적관계.방법 채용병례-대조연구방법,응용형광탐침실시정량PCR법대2009지2012년재상해모의원취진적632례HCC환자화723명비HCC을간병독(HBV)감염자적STAT3기인계동자구-1096G/C기인다태성진행검측,사용단인소분석방법학정해기인다태성OR(95％ CI)치.결과 대조조、병례조휴대STAT3-1096C(GC+ CC)기인형자분별점61.8％ (447/723)、60.6％(383/632),불동기인형지간HCC발병풍험차이무통계학의의(OR =0.95,95％ CI:0.76～1.18).이성별진행분층분석,재남성인군중,대조조、병례조휴대STAT3-1096 C(GC+ CC)기인형자분별점62.2％(314/505)、61.8％(331/536),재녀성인군중,분별위61.0％(133/218)、54.17％ (52/96),재남성화녀성인군중,불동기인형HCC발병풍험차이균몰유통계학의의(OR=0.98,95％ CI:0.77～1.26;OR =0.76,95％CI:0.47 ～ 1.26).이HBV기인형진행분층분석,재HBV B형감염자중,대조조、병례조휴대STAT3-1096C(GC+ CC)기인형자분별점61.5％(110/179)、53.1％(34/64),재HBV C형감염자중,대조조、병례조휴대STAT3-1096C(GC+CC)기인형자분별점62.9％(276/439)、60.3％(226/375),재HBV B혹C형감염자중,불동기인형환자HCC발병풍험차이몰유통계학의의(OR =0.71,95％ CI:0.40～1.26;OR =0.90,95％ CI:0.68～ 1.19).결론 STAT3-1096G/C기인다태성여HBV양성HCC적역감성몰유상관성.
Objective To evaluate the association of signal transducer and activators of transcription 3 (STAT3)-1096G/C polymorphism in promoter region with the susceptibility to HBsAg positive hepatocellular carcinoma (HCC).Methods A total of 632 patients with HCC and 723 HBV-infected subjects without HCC treated at Changhai Hospital of Shanghai from 2009 to 2012 were included in this casecontrol study.The polymorphism of STAT3-1096 G/C was genotyped by Fluorescent probe-Real time quantitative PCR.Univariate analysis was used to calculate the odds ratio (OR) and its 95％ confidence interval (CI).Results The frequency of genetic allele STAT3-1096G/C (GC + CC) of control group and case group were 61.83％ (447/723) and 60.60％ (383/632),while difference of HCC risk was not found among different genotypes (OR =0.95,95％ CI:0.76-1.18).When stratified by sex,the frequency of genetic allele STAT3-1096C (GC + CC) of control group and case group were 62.18％ (314/505) and 61.75％ (331/536) in men,61.01％ (133/218) and 54.17％ (52/96) in women,respectively,while difference of HCC risk was not found among different genotypes (OR =0.98,95％ CI:0.77-1.26 ; OR =0.76,95％ CI:0.47-1.26,respectively).When stratified by HBV genotypes,the frequency of genetic allele STAT3-1096C (GC + CC) of control group and case group were 61.45％ (110/179) and 53.13％ (34/64) in HBV genotype B,62.87％ (276/439) and 60.27％ (226/375) in HBV genotype C,respectively,while difference of HCC risk was not found among different genotypes (OR =0.71,95％ CI:0.40-1.26; OR =0.90,95％ CI:0.68-1.19,respectively).Conclusion STAT3-1096G/C polymorphism was not associated with the susceptibility to HCC for the HBV-infected subjects without HCC.
