中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2014年
8期
699-704
,共6页
贾华云%高立冬%郭云昌%李薇薇%王岚%陈帅%张红
賈華雲%高立鼕%郭雲昌%李薇薇%王嵐%陳帥%張紅
가화운%고입동%곽운창%리미미%왕람%진수%장홍
沙门菌属%电泳,凝胶,脉冲场%食品污染%动物源性食品%药敏试验
沙門菌屬%電泳,凝膠,脈遲場%食品汙染%動物源性食品%藥敏試驗
사문균속%전영,응효,맥충장%식품오염%동물원성식품%약민시험
Salmonella%Electrophoresis,gel,pulsed-field%Food contamination%Animal source foods%Antibiotic susceptibility test
目的 研究湖南省动物源性食品中沙门菌的分子流行病学特征.方法 2010年在湖南省10个市的集贸市场和超市,按照无菌采样原则采集畜肉159份、禽肉152份,动物性水产品381份,共计692份样品,分离沙门菌,进行血清、耐药表型和PFGE分子分型.结果 692份动物源性食品中,93份样品检出沙门菌,检出率为13.4％,禽肉、畜肉和水产品中沙门菌检出率分别为23.0％ (35/152),22.6％ (36/159)和5.8％(22/381),水产品中沙门菌检出率低于禽肉和畜肉(x2值分别为33.86、33.29,P值均＜0.05).分离菌株血清型呈多样化分布,优势血清型为德尔卑沙门菌,占35.1％(33/94).79.8％ (75/94)的菌株至少对一种抗生素耐药,31.9％ (30/94)的菌株对5种以上抗生素耐药,呈多重耐药性,禽、畜肉中分离菌株的多重耐药率分别为47.2％ (17/36)和22.2％(8/36),禽肉分离的多重耐药菌株高于畜肉(x2=4.96,P＜0.05).试验菌株对四环素的耐药率最高,达62.8％(59/94).PFGE分型可以分为69个PFGE型,优势型为7型(6株)、15型(4株)和22型(6株).结论 湖南省动物源性食品沙门菌污染严重,且菌株耐药形势严峻,PFGE分型结果提示可能存在沙门菌流行株.
目的 研究湖南省動物源性食品中沙門菌的分子流行病學特徵.方法 2010年在湖南省10箇市的集貿市場和超市,按照無菌採樣原則採集畜肉159份、禽肉152份,動物性水產品381份,共計692份樣品,分離沙門菌,進行血清、耐藥錶型和PFGE分子分型.結果 692份動物源性食品中,93份樣品檢齣沙門菌,檢齣率為13.4％,禽肉、畜肉和水產品中沙門菌檢齣率分彆為23.0％ (35/152),22.6％ (36/159)和5.8％(22/381),水產品中沙門菌檢齣率低于禽肉和畜肉(x2值分彆為33.86、33.29,P值均＜0.05).分離菌株血清型呈多樣化分佈,優勢血清型為德爾卑沙門菌,佔35.1％(33/94).79.8％ (75/94)的菌株至少對一種抗生素耐藥,31.9％ (30/94)的菌株對5種以上抗生素耐藥,呈多重耐藥性,禽、畜肉中分離菌株的多重耐藥率分彆為47.2％ (17/36)和22.2％(8/36),禽肉分離的多重耐藥菌株高于畜肉(x2=4.96,P＜0.05).試驗菌株對四環素的耐藥率最高,達62.8％(59/94).PFGE分型可以分為69箇PFGE型,優勢型為7型(6株)、15型(4株)和22型(6株).結論 湖南省動物源性食品沙門菌汙染嚴重,且菌株耐藥形勢嚴峻,PFGE分型結果提示可能存在沙門菌流行株.
목적 연구호남성동물원성식품중사문균적분자류행병학특정.방법 2010년재호남성10개시적집무시장화초시,안조무균채양원칙채집축육159빈、금육152빈,동물성수산품381빈,공계692빈양품,분리사문균,진행혈청、내약표형화PFGE분자분형.결과 692빈동물원성식품중,93빈양품검출사문균,검출솔위13.4％,금육、축육화수산품중사문균검출솔분별위23.0％ (35/152),22.6％ (36/159)화5.8％(22/381),수산품중사문균검출솔저우금육화축육(x2치분별위33.86、33.29,P치균＜0.05).분리균주혈청형정다양화분포,우세혈청형위덕이비사문균,점35.1％(33/94).79.8％ (75/94)적균주지소대일충항생소내약,31.9％ (30/94)적균주대5충이상항생소내약,정다중내약성,금、축육중분리균주적다중내약솔분별위47.2％ (17/36)화22.2％(8/36),금육분리적다중내약균주고우축육(x2=4.96,P＜0.05).시험균주대사배소적내약솔최고,체62.8％(59/94).PFGE분형가이분위69개PFGE형,우세형위7형(6주)、15형(4주)화22형(6주).결론 호남성동물원성식품사문균오염엄중,차균주내약형세엄준,PFGE분형결과제시가능존재사문균류행주.
Objective To study the molecular epidemiological characteristics of Salmonella in animal source foods in Hunan.Methods The fair trade markets and supermarkets of ten cities were chosen to sample animal source foods for isolating Salmonella in Hunan province in 2010.A total of 692 samples were collected by aseptic sampling,included 159 livestock meats,152 poultry meats,and 381 aquatic products.Salmonella strains isolated were subjected to stereotyping,antimicrobial susceptibility testing and pulsed field gel electrophoresis (PFGE).Results Salmonella was detected in 93 of 692 animal food samples with the detection rate of 13.4％.The detection rates of Salmonella in poultry meats,livestock meats and aquatic products were 23.0％ (35/152),22.6％ (36/159) and 5.8％ (22/381) respectively.Therefore,the detection rate in aquatic products was lower than that of poultry meats and livestock meats (x2 =33.86,P ＜ 0.05 ; x2 =33.29,P ＜ 0.05,respectively).The serotypes of isolates showed diversity,and Salmonella Derby(33/94,35.1％) was the predominant serotypes.79.8％ (75/94) strains showed resistant to more than one antibiotic used in the test,31.9％ (30/94) strains showed resistant to more than 5 antibiotics.A significant difference was observed for multidrug resistance between Salmonella isolated from poultry(47.2％,17/36) and livestock meats (22.2％,8/36) (x2 =4.96,P ＜ 0.05).And the highest resistant rate was found in tetracycline,as high as 62.8％ (59/94).All the strains were divided into 69 PFGE subtypes.Furthermore the dominating subtypes were type 7 (6 strains),type 15 (4 strains),type 22 (6 strains).Conclusion Inspection results showed that Salmonella contamination in animal source foods were serious in Hunan province,and the isolates expressed high level resistance to the antibiotics.Furthermore the PFGE results indicated that there were epidemic strains of Salmonella in Hunan.
