中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2014年
10期
893-899
,共7页
冯晶%黄素丽%何美安%戴霞云%李珺%邓棋霏%邱高坤%杨彬珧%邬堂春
馮晶%黃素麗%何美安%戴霞雲%李珺%鄧棋霏%邱高坤%楊彬珧%鄔堂春
풍정%황소려%하미안%대하운%리군%산기비%구고곤%양빈요%오당춘
多态性,单核苷酸%横断面研究%microRNA-320b%冠心病
多態性,單覈苷痠%橫斷麵研究%microRNA-320b%冠心病
다태성,단핵감산%횡단면연구%microRNA-320b%관심병
Polymorphism,single nucleotide%Cross-sectional studies%microRNA-320b%Coronary heart disease
目的 探讨中国汉族人群miRNA前体-320b-2 (pre-miR-320b-2)启动子区单核苷酸多态性(rs10916581)对冠心病易感性的关联性及对血浆microRNA-320b(miR-320b)表达水平的影响,并探索可能影响血浆miR-320b表达水平的因素.方法 采用病例-对照研究方法,于2004年5月至2009年10月应用单纯抽样法随机法选择入住武汉同济医院、协和医院和武钢总医院3个医院冠心病患者1 507例和与之年龄、性别频率匹配的1 379名健康对照为研究对象,对研究对象的rs10916581位点进行基因分型.从上述人群中采用单纯随机法选择无急性感染疾病的174例冠心病患者,并选择匹配的无急性感染疾病无糖尿病的181名健康对照,应用RT-PCR法检测其血浆miR-320b表达水平.采用多元非条件logistic回归分析rs10916581与冠心病易感性的关联,采用广义线性回归分析各因素与血浆miR-320b表达水平的关联.结果 在单因素logistic回归模型中,rs10916581与冠心病易感性的关联性无统计学意义(P值均＞0.05).在校正年龄、性别、BMI、吸烟状态、糖尿病、高血压、TC/HDL-C比值、总TG后,rs10916581与冠心病易感性的关联性仍无统计学意义[与CC基因型相比,CT、TT、CT+TT基因型患冠心病的OR (95％ CI)值分别为0.94(0.76 ～ 1.15)、0.99(0.74～1.33)和0.95(0.78～ 1.16)].年龄、性别、吸烟状态、BMI、高血压、糖尿病、冠心病家族史和rs10916581在冠心病的发生中无交互作用(P交互值均＞0.05).在对照、病例和总人群中,rs10916581均不影响血浆miR-320b的表达水平[β(95％ CI)值分别为:-0.028(-0.495 ～ 0.440)、0.250(-0.226 ～0.727)、0.134(-0.218 ～0.486),P值均＞0.05].但在病例组中,血浆miR-320b表达水平与BMI负相关[β(95％ CI)值为-0.140(-0.261 ～-0.020),P=0.022],与TC/HDL-C值正相关[β(95％ CI)值为0.620(0.261～0.979),P=0.001],总人群中血浆miR-β20b表达水平与TC/HDL-C值正相关[β(95％ CI)值为0.108(0.027～0.190),P=0.009],与糖尿病、高血压患病状态则呈负相关[β(95％ CI)值分别为-1.025(-1.696 ～-0.354)、-0.594(-1.138～-0.049),P值均＜0.05].结论 pre-miR-320b-2启动子区rs10916581与冠心病易感性和血浆miR-320b表达水平无关联,冠心病患者的BMI、TC/HDL-C值、糖尿病和高血压患病状态可能影响血浆miR-320b的表达水平.
目的 探討中國漢族人群miRNA前體-320b-2 (pre-miR-320b-2)啟動子區單覈苷痠多態性(rs10916581)對冠心病易感性的關聯性及對血漿microRNA-320b(miR-320b)錶達水平的影響,併探索可能影響血漿miR-320b錶達水平的因素.方法 採用病例-對照研究方法,于2004年5月至2009年10月應用單純抽樣法隨機法選擇入住武漢同濟醫院、協和醫院和武鋼總醫院3箇醫院冠心病患者1 507例和與之年齡、性彆頻率匹配的1 379名健康對照為研究對象,對研究對象的rs10916581位點進行基因分型.從上述人群中採用單純隨機法選擇無急性感染疾病的174例冠心病患者,併選擇匹配的無急性感染疾病無糖尿病的181名健康對照,應用RT-PCR法檢測其血漿miR-320b錶達水平.採用多元非條件logistic迴歸分析rs10916581與冠心病易感性的關聯,採用廣義線性迴歸分析各因素與血漿miR-320b錶達水平的關聯.結果 在單因素logistic迴歸模型中,rs10916581與冠心病易感性的關聯性無統計學意義(P值均＞0.05).在校正年齡、性彆、BMI、吸煙狀態、糖尿病、高血壓、TC/HDL-C比值、總TG後,rs10916581與冠心病易感性的關聯性仍無統計學意義[與CC基因型相比,CT、TT、CT+TT基因型患冠心病的OR (95％ CI)值分彆為0.94(0.76 ～ 1.15)、0.99(0.74～1.33)和0.95(0.78～ 1.16)].年齡、性彆、吸煙狀態、BMI、高血壓、糖尿病、冠心病傢族史和rs10916581在冠心病的髮生中無交互作用(P交互值均＞0.05).在對照、病例和總人群中,rs10916581均不影響血漿miR-320b的錶達水平[β(95％ CI)值分彆為:-0.028(-0.495 ～ 0.440)、0.250(-0.226 ～0.727)、0.134(-0.218 ～0.486),P值均＞0.05].但在病例組中,血漿miR-320b錶達水平與BMI負相關[β(95％ CI)值為-0.140(-0.261 ～-0.020),P=0.022],與TC/HDL-C值正相關[β(95％ CI)值為0.620(0.261～0.979),P=0.001],總人群中血漿miR-β20b錶達水平與TC/HDL-C值正相關[β(95％ CI)值為0.108(0.027～0.190),P=0.009],與糖尿病、高血壓患病狀態則呈負相關[β(95％ CI)值分彆為-1.025(-1.696 ～-0.354)、-0.594(-1.138～-0.049),P值均＜0.05].結論 pre-miR-320b-2啟動子區rs10916581與冠心病易感性和血漿miR-320b錶達水平無關聯,冠心病患者的BMI、TC/HDL-C值、糖尿病和高血壓患病狀態可能影響血漿miR-320b的錶達水平.
목적 탐토중국한족인군miRNA전체-320b-2 (pre-miR-320b-2)계동자구단핵감산다태성(rs10916581)대관심병역감성적관련성급대혈장microRNA-320b(miR-320b)표체수평적영향,병탐색가능영향혈장miR-320b표체수평적인소.방법 채용병례-대조연구방법,우2004년5월지2009년10월응용단순추양법수궤법선택입주무한동제의원、협화의원화무강총의원3개의원관심병환자1 507례화여지년령、성별빈솔필배적1 379명건강대조위연구대상,대연구대상적rs10916581위점진행기인분형.종상술인군중채용단순수궤법선택무급성감염질병적174례관심병환자,병선택필배적무급성감염질병무당뇨병적181명건강대조,응용RT-PCR법검측기혈장miR-320b표체수평.채용다원비조건logistic회귀분석rs10916581여관심병역감성적관련,채용엄의선성회귀분석각인소여혈장miR-320b표체수평적관련.결과 재단인소logistic회귀모형중,rs10916581여관심병역감성적관련성무통계학의의(P치균＞0.05).재교정년령、성별、BMI、흡연상태、당뇨병、고혈압、TC/HDL-C비치、총TG후,rs10916581여관심병역감성적관련성잉무통계학의의[여CC기인형상비,CT、TT、CT+TT기인형환관심병적OR (95％ CI)치분별위0.94(0.76 ～ 1.15)、0.99(0.74～1.33)화0.95(0.78～ 1.16)].년령、성별、흡연상태、BMI、고혈압、당뇨병、관심병가족사화rs10916581재관심병적발생중무교호작용(P교호치균＞0.05).재대조、병례화총인군중,rs10916581균불영향혈장miR-320b적표체수평[β(95％ CI)치분별위:-0.028(-0.495 ～ 0.440)、0.250(-0.226 ～0.727)、0.134(-0.218 ～0.486),P치균＞0.05].단재병례조중,혈장miR-320b표체수평여BMI부상관[β(95％ CI)치위-0.140(-0.261 ～-0.020),P=0.022],여TC/HDL-C치정상관[β(95％ CI)치위0.620(0.261～0.979),P=0.001],총인군중혈장miR-β20b표체수평여TC/HDL-C치정상관[β(95％ CI)치위0.108(0.027～0.190),P=0.009],여당뇨병、고혈압환병상태칙정부상관[β(95％ CI)치분별위-1.025(-1.696 ～-0.354)、-0.594(-1.138～-0.049),P치균＜0.05].결론 pre-miR-320b-2계동자구rs10916581여관심병역감성화혈장miR-320b표체수평무관련,관심병환자적BMI、TC/HDL-C치、당뇨병화고혈압환병상태가능영향혈장miR-320b적표체수평.
Objective To investigate the effects of rs10916581,a common single nucleotide polymorphism (SNP) located in the promoter region of pre-miR-320b-2,on coronary heart disease (CHD) risk and circulating microRNA-320b (miR-320b) level.To explore potential factors influencing circulating miR-320b level.Methods Rs10916581 was genotyped in a case-control study with 1 507 CHD cases and 1 379 age-and sex-frequency-matched controls.The cases were consecutively recruited from 3 hospitals (Tongji Hospital,Union Hospital,and Wugang Hospital) in Wuhan city (Hubei,China) between May 2004 and October 2009 and all the controls resided in Wuhan communities.A subgroup of 174 CHD cases and 181 non-diabetes controls without acute infection were randomly selected and their circulating miR-320b levels were detected using quantitative reverse transcriptase polymerase chain reaction assays.The association of rs10916581 with CHD susceptibility was analyzed with multivariable logistic regression model.Generalized linear regression model was used to explore the associations of rs10916581 and some other factors with circulating miR-320b level.Results In single-factor logistic regression analysis,no association was found between rs10916581 and CHD risk.After adjustment for age,sex,BMI,smoking status,hypertension,diabetes,total triglyceride,total cholesterol/high density lipoprotein (TC/HDL-C),the result did not materially alter(compared with CC genotype,the OR(95％ CI) of CHR in the subjects carried CT,TT,CT + TT genotypes were 0.94 (0.76-1.15),0.99 (0.74-1.33) and 0.95 (0.78-1.16)).No significant interactions were observed between the conventional risk factors of CHD (age,gender,smoking status,BMI,hypertension,diabetes,CHD family history) and rs10916581 on CHD risk (P ＞0.05).Rs10916581 showed no significant association with circulating miR-320b level in cases,controls or total population (β(95％CI) was-0.028 (-0.495-0.440),0.250 (-0.226-0.727) and 0.134 (-0.218-0.486) respectively,P ＞ 0.05).However,circulating miR-320b level was negatively associated with BMI (β(95％ CI)was-0.140 (-0.261--0.020),P =0.022) while positively associated with TC/HDL (β (95 ％ CI) was 0.620 (0.261-0.979),P =0.001) in cases,and in total population,its circulating level tended to be lower in diabetes or hypertension patients (β(95％ CI) was-1.025 (-1.696--0.354) and -0.594 (-1.138--0.049) respectively,P =0.003,0.033 respectively) and was positively associated with TC/HDL-C (β(95％CI) was 0.108 (0.027-0.190),P =0.009).Conclusion The common SNP (rs10916581) in the promoter region of pre-miR-320b-2 might have little contribution to the CHD predisposition in Chinese Han population,and it might not affect circulating miR-320b level.Conventional CHD risk factors (BMI,TC/HDL-C,hypertension and diabetes) might have effects on its circulating level.
