中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2012年
12期
1102-1106
,共5页
宋虎平%雷春灵%惠延年%王建洲%雷晓琴%艾华
宋虎平%雷春靈%惠延年%王建洲%雷曉琴%艾華
송호평%뢰춘령%혜연년%왕건주%뢰효금%애화
糖尿病视网膜病变%糖尿病,实验性%神经损伤诱导蛋白-1%髓样细胞%细胞黏附
糖尿病視網膜病變%糖尿病,實驗性%神經損傷誘導蛋白-1%髓樣細胞%細胞黏附
당뇨병시망막병변%당뇨병,실험성%신경손상유도단백-1%수양세포%세포점부
Diabetic retinopathy%Diabetes mellitus,experiment%Ninjurin-1%Myeloid Cells%Adhesion
目的 观察神经损伤诱导蛋白(Ninj)-1对早期糖尿病大鼠视网膜髓样细胞黏附的调节作用.方法 实验研究.通过链脲佐菌素腹腔内注射诱导建立大鼠糖尿病模型.建模成功后2个月,取27只大鼠随机分为糖尿病+磷酸盐缓冲液(PBS)腹腔内注射组(B组)、糖尿病+抗Ninj-1腹腔内注射组(C组)、糖尿病+ IgG腹腔内注射组(D组),每组9只大鼠.取9只月龄匹配的正常大鼠作为对照组(A组).分别以丫啶橙视网膜白细胞造影和髓过氧化物酶(MPO)酶联免疫吸附试剂盒检测视网膜白细胞黏附数量及髓样细胞活性.4组间均数的比较采用单因素方差分析,均数间差异的多重比较采用LSD-t检验.结果 丫啶橙视网膜造影检测结果显示,A、B、C、D组大鼠视网膜白细胞数量分别为(49.66±13.51)、(153.66±20.43)、(85.33±15.03)、(156.33±11.53)个,差异有统计学意义(F=143.34,P=0.000),C组大鼠视网膜白细胞数量显著低于B组(P=0.000,95％可信区间为-82.68～-53.98).4组大鼠视网膜上清液中MPO的浓度分别为(15.66±2.08)、(27.66±2.51)、(18.02±2.01)、(26.66±3.21) μg/L,差异有统计学意义(F=17.61,P=0.010),C组大鼠视网膜组织中MPO水平显著低于B组(P=0.010,95％可信区间为-14.37～-4.95).结论 在体内实验条件下,Ninj-1可能对早期糖尿病大鼠视网膜髓样细胞黏附有一定的调节作用.
目的 觀察神經損傷誘導蛋白(Ninj)-1對早期糖尿病大鼠視網膜髓樣細胞黏附的調節作用.方法 實驗研究.通過鏈脲佐菌素腹腔內註射誘導建立大鼠糖尿病模型.建模成功後2箇月,取27隻大鼠隨機分為糖尿病+燐痠鹽緩遲液(PBS)腹腔內註射組(B組)、糖尿病+抗Ninj-1腹腔內註射組(C組)、糖尿病+ IgG腹腔內註射組(D組),每組9隻大鼠.取9隻月齡匹配的正常大鼠作為對照組(A組).分彆以丫啶橙視網膜白細胞造影和髓過氧化物酶(MPO)酶聯免疫吸附試劑盒檢測視網膜白細胞黏附數量及髓樣細胞活性.4組間均數的比較採用單因素方差分析,均數間差異的多重比較採用LSD-t檢驗.結果 丫啶橙視網膜造影檢測結果顯示,A、B、C、D組大鼠視網膜白細胞數量分彆為(49.66±13.51)、(153.66±20.43)、(85.33±15.03)、(156.33±11.53)箇,差異有統計學意義(F=143.34,P=0.000),C組大鼠視網膜白細胞數量顯著低于B組(P=0.000,95％可信區間為-82.68～-53.98).4組大鼠視網膜上清液中MPO的濃度分彆為(15.66±2.08)、(27.66±2.51)、(18.02±2.01)、(26.66±3.21) μg/L,差異有統計學意義(F=17.61,P=0.010),C組大鼠視網膜組織中MPO水平顯著低于B組(P=0.010,95％可信區間為-14.37～-4.95).結論 在體內實驗條件下,Ninj-1可能對早期糖尿病大鼠視網膜髓樣細胞黏附有一定的調節作用.
목적 관찰신경손상유도단백(Ninj)-1대조기당뇨병대서시망막수양세포점부적조절작용.방법 실험연구.통과련뇨좌균소복강내주사유도건립대서당뇨병모형.건모성공후2개월,취27지대서수궤분위당뇨병+린산염완충액(PBS)복강내주사조(B조)、당뇨병+항Ninj-1복강내주사조(C조)、당뇨병+ IgG복강내주사조(D조),매조9지대서.취9지월령필배적정상대서작위대조조(A조).분별이아정등시망막백세포조영화수과양화물매(MPO)매련면역흡부시제합검측시망막백세포점부수량급수양세포활성.4조간균수적비교채용단인소방차분석,균수간차이적다중비교채용LSD-t검험.결과 아정등시망막조영검측결과현시,A、B、C、D조대서시망막백세포수량분별위(49.66±13.51)、(153.66±20.43)、(85.33±15.03)、(156.33±11.53)개,차이유통계학의의(F=143.34,P=0.000),C조대서시망막백세포수량현저저우B조(P=0.000,95％가신구간위-82.68～-53.98).4조대서시망막상청액중MPO적농도분별위(15.66±2.08)、(27.66±2.51)、(18.02±2.01)、(26.66±3.21) μg/L,차이유통계학의의(F=17.61,P=0.010),C조대서시망막조직중MPO수평현저저우B조(P=0.010,95％가신구간위-14.37～-4.95).결론 재체내실험조건하,Ninj-1가능대조기당뇨병대서시망막수양세포점부유일정적조절작용.
Objective To investigate the regulatory effects of ninjurin-1 on adhesion of myeloid cells in the retina at the early stage of diabetic rats.Methods Experimental study.The rat diabetic model was induced by intraperitoneal injection of streptozotocin.After 2 months of diabetes induction,27 diabetic rats were randomly chosen and assigned to 3 groups,including diabetes and phosphate buffered saline(PBS)injection group (group B),diabetes and anti-Ninj-1 injection group (group C) and diabetes and anti-IgG injection group (group D),with 9 rats in each group.Nine age matched health rats were chosen as control group(group A).Retinal leukostasis was quantified with acridine orange leukocyte fluorography.Retinal myeloid cell infiltration activity was measured by enzyme linked immunosorbent assay of myeloperoxidase (MPO).The differences of the mean values among the four groups were analyzed by one-factor analysis of variance.The multiple comparisons of the mean values among the four groups were analyzed by LSD-t analysis.Results According to the results of the acridine orange leukocyte fluorography,the numbers of leukocyte adhesion in the four groups were 49.66±13.51,153.66±20.43,85.33±15.03 and 156.33±11.53,respectively.The differences among them were significant(F=143.34,P =0.000).The numbers of leukocyte adhesion in the group C were significantly lower than that in group B (P =0.000,95％ CI:-82.68--53.98).The levels of retinal MPO in the four groups were (15.66 ± 2.08),(27.66 ± 2.51),(18.02 ± 2.01) and (26.66 ± 3.21) μg/L,respectively.The differences among them were significant(F =17.61,P =0.010).The level of retinal MPO in the group C was significantly lower than that in group B(P =0.010,95％ CI:-14.37--4.95).Conclusions Ninj-1 may play a role in the mediation of the adhesion of myeloid cell to the rat retina of early-stage of diabetes in vivo.
