早产对大鼠视网膜血管形态发育的影响
조산대대서시망막혈관형태발육적영향
Effect of premature birth on retinal vascular development in the neonatal rat
  • 万方数据
    中华眼科杂志 중화안과잡지
    Chinese Journal of Ophthalmology
    2013年 8期 729-735 ,共7页

    杨湘敏%李蓉%王雨生%储昭节%高翔

    양상민%리용%왕우생%저소절%고상

    早产%视网膜血管%生长和发育%动物,新生%大鼠 早產%視網膜血管%生長和髮育%動物,新生%大鼠
    조산%시망막혈관%생장화발육%동물,신생%대서
    Premature birth%Retinal vessels%Growth and development%Animals,newborn%Rats
    目的 探讨早产对SD大鼠视网膜血管形态发育的影响.方法 实验研究.将60只受孕的SD雌鼠分为4组:细菌脂多糖诱导早产组(LPS组)、米非司酮诱导早产组(RP组)和剖宫产早产组(CP组),正常生产大鼠为对照组.记录各组幼鼠出生后21 d内体重增长情况.在生后4、7、10、14 d随机取各组幼鼠右眼进行视网膜铺片,观察血管生长状况.根据出生幼鼠只数不同,在3个早产组内又分为小窝别(每窝产6～8只幼鼠者,即1组)和大窝别(每窝产14～ 16只幼鼠者,即2组)两个亚组,在出生后4、7、10d每个亚组随机取6只幼鼠的右眼,观察视网膜血管生长情况.计量资料的比较采用t检验,多组计量资料的比较采用方差分析,组间的多重比较采用LSD-t检验.结果 LPS组、CP组、RP组幼鼠出生21 d内体重均低于对照组,差异有统计学意义(LSD-t检验:P值均＜0.05).LPS组[(0.47±0.02)％、(0.63±0.04)％]和RP组[(0.49±0.04)％、(0.65±0.04)％]幼鼠生后4d和7d的视网膜血管化比例小于对照组[(0.57±0.04)％、(0.74±0.05)％](LPS组:t4d =6.427,P4d=0.000; t7d=5.111,P7d=0.000; RP组:t4d=4.469,P4 d=0.000; t7d=2.491,p7d=0.022)；CP组幼鼠生后4、7、10 d的视网膜血管化比例[(0.49±0.05)％、(0.61±0.05)％、(0.94±0.03)％]均小于对照组[(0.57±0.04)％、(0.74±0.05)％、(0.97±0.02)％](t4d =4.044,P4d =0.001；t7d =6.011,P7d =0.000;t10d =2.331,P10d=0.030).生后14 d各组幼鼠视网膜表层血管化均已完成.LPS组内LPS2组幼鼠生后4d和7d的视网膜血管化比例[(0.44±0.02)％、(0.60±0.03)％]小于LPS1组[(0.53±0.04)％、(0.74±0.03)％] (t4d=3.852,P4d=0.008；t7 d=5.630,P7d =0.001)；CP组内CP2组幼鼠生后4d和7d的视网膜血管化比例[(0.43±0.02)％、(0.64±0.03)％]小于CP1组[(0.54±0.03)％、(0.76±0.02)％](t4 d=4.695,P4d=0.003；t7d =6.025;P7 d=0.001)；RP组内RP2组幼鼠生后4d的视网膜血管化比例[(0.44±0.01)％]小于RP1组[(0.54±0.04)％](t4d=5.000,P4d=0.002).结论 相比正常幼鼠,早产幼鼠在生后早期体重增长较慢,视网膜血管化成熟进程较慢；早产大窝幼鼠较小窝幼鼠视网膜血管化比例小.提示早产和大窝别可能影响新生大鼠早期视网膜血管发育进程.
    목적 탐토조산대SD대서시망막혈관형태발육적영향.방법 실험연구.장60지수잉적SD자서분위4조:세균지다당유도조산조(LPS조)、미비사동유도조산조(RP조)화부궁산조산조(CP조),정상생산대서위대조조.기록각조유서출생후21 d내체중증장정황.재생후4、7、10、14 d수궤취각조유서우안진행시망막포편,관찰혈관생장상황.근거출생유서지수불동,재3개조산조내우분위소와별(매와산6～8지유서자,즉1조)화대와별(매와산14～ 16지유서자,즉2조)량개아조,재출생후4、7、10d매개아조수궤취6지유서적우안,관찰시망막혈관생장정황.계량자료적비교채용t검험,다조계량자료적비교채용방차분석,조간적다중비교채용LSD-t검험.결과 LPS조、CP조、RP조유서출생21 d내체중균저우대조조,차이유통계학의의(LSD-t검험:P치균＜0.05).LPS조[(0.47±0.02)％、(0.63±0.04)％]화RP조[(0.49±0.04)％、(0.65±0.04)％]유서생후4d화7d적시망막혈관화비례소우대조조[(0.57±0.04)％、(0.74±0.05)％](LPS조:t4d =6.427,P4d=0.000; t7d=5.111,P7d=0.000; RP조:t4d=4.469,P4 d=0.000; t7d=2.491,p7d=0.022)；CP조유서생후4、7、10 d적시망막혈관화비례[(0.49±0.05)％、(0.61±0.05)％、(0.94±0.03)％]균소우대조조[(0.57±0.04)％、(0.74±0.05)％、(0.97±0.02)％](t4d =4.044,P4d =0.001；t7d =6.011,P7d =0.000;t10d =2.331,P10d=0.030).생후14 d각조유서시망막표층혈관화균이완성.LPS조내LPS2조유서생후4d화7d적시망막혈관화비례[(0.44±0.02)％、(0.60±0.03)％]소우LPS1조[(0.53±0.04)％、(0.74±0.03)％] (t4d=3.852,P4d=0.008；t7 d=5.630,P7d =0.001)；CP조내CP2조유서생후4d화7d적시망막혈관화비례[(0.43±0.02)％、(0.64±0.03)％]소우CP1조[(0.54±0.03)％、(0.76±0.02)％](t4 d=4.695,P4d=0.003；t7d =6.025;P7 d=0.001)；RP조내RP2조유서생후4d적시망막혈관화비례[(0.44±0.01)％]소우RP1조[(0.54±0.04)％](t4d=5.000,P4d=0.002).결론 상비정상유서,조산유서재생후조기체중증장교만,시망막혈관화성숙진정교만；조산대와유서교소와유서시망막혈관화비례소.제시조산화대와별가능영향신생대서조기시망막혈관발육진정.
    Objective To study the effects of premature birth on the development of rat retinal vasculature.Methods Experimental study.Sixty pregnant Sprague-Dawley rats were divided into four groups:bacterial lipopolysaccharide-induced preterm group (LPS group),RU-486 induced preterm group (RP group),cesarean section induced preterm group (CP group),and the normal delivery rats as the control group.The weight of rats from each group was recorded until postnatal day 21.On postnatal day 4,7,10 and 14 (P4,P7,P10 and P14),the retina of right eye was dissected and whole-mounted.Each premature group was divided into two subgroups based on the number of rats in each litter,the small subgroup (6-8 rats per litter,group 1) and the large subgroup (14-18 rats per litter,group 2).The development of retinal vascularization process was observed on P4,P7 and P10 (n =6).Independent t test,one-way ANOVA and LSD-t test were used to analyzed the results.Results The weight of premature rats in LPS,CP and RP groups was significantly lower than that in the normal group within postnatal 21 days (LSD-t test:all P ＜0.05).On the P4 and P7 in LPS group,the proportions of retinal superficial vascularized area of newborn rats [(0.47 ± 0.02) ％,(0.63 ± 0.04) ％] were less than that in the normal group [(0.57 ± 0.04) ％,(0.74 ±0.05)％] (t4d =6.427,P4d =0.000;t7d =5.111,P7d =0.000).On the P4 and P7 in RP group,this proportions [(0.49 ± 0.04) ％,(0.65 ± 0.04) ％] were less than that in the normal group [(0.57 ±0.04) ％,(0.74 ± 0.05) ％] (t4 d =4.469,P4 d =0.000 ; t7 d =2.491,P7 d =0.022).On P4,P7 and P10 in CP group,this proportions [(0.49 ± 0.05) ％,(0.61 ± 0.05) ％,(0.94 ± 0.03) ％] were also less than that in the normal group[(0.57 ±0.04)％,(0.74 ±0.05)％,(0.97 ±0.02)％] (qd =4.044,P4d =0.001 ;t7d =6.011,P7 d =0.000 ; t 10 d =2.331,P10 d =0.030).Retinal superficial vascularization completed on P14 in all groups.On the P4 and P7 in LPS group,the proportion of retinal vascularized area of group 2 [(0.44±0.02)％,(0.60±0.03)％] were less than that of group 1 [(0.53 ±0.04)％,(0.74±0.03)％] (t4d =3.852,P4d =0.008;t7d =5.630,P7d =0.001).On the P4 and P7 in CP group,this proportion in group 2 [(0.43 ± 0.02) ％,(0.64 ± 0.03) ％] were less than that of group 1 [(0.54 ± 0.03) ％,(0.76 ± 0.02) ％] (t4 d =4.695,P4d =0.003 ; t7 d =6.025,P7 d =0.001).On P4 in RP group,the proportions of group 2 [(0.44 ± 0.01) ％] was less than that of group 1 [(0.54 ± 0.04) ％] (t4d =5.000,P4d =0.002).Conclusions The premature rats have lower weight and much slower rate of early retinal vascularization,as compared with the normal rats.Furthermore,in the premature rats,the proportion of retinal vascularization in larger litters is less than that in smaller litters.These results indicate that premature birth and larger litter size have effects on the development of rat retinal vasculature.
    원문보기 원문다운로드 사이트로이동
저자의 최근 논문