中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2009年
39期
2797-2801
,共5页
高文清%李选%刘鹏程%白智勇%钟延丰%韩鸿滨%李敬府%刘伟%郑卓肇%隋玉洁%马小龙%吕献军%徐辉%曾祥柱
高文清%李選%劉鵬程%白智勇%鐘延豐%韓鴻濱%李敬府%劉偉%鄭卓肇%隋玉潔%馬小龍%呂獻軍%徐輝%曾祥柱
고문청%리선%류붕정%백지용%종연봉%한홍빈%리경부%류위%정탁조%수옥길%마소룡%려헌군%서휘%증상주
超声检查%磁共振成像%超顺磁氧化铁
超聲檢查%磁共振成像%超順磁氧化鐵
초성검사%자공진성상%초순자양화철
Ultrasonography%Magnetic resonance imaging%SPIO
目的 探索血池内造影剂Sonovue和Feridex进入血管壁的可能性.方法 普通级雄性日本大耳白兔17只,11只高脂饲养动脉粥样硬化(AS)模型,6只普食正常对照.数字随机取AS组10只和正常组6只,用和不用超声介导微泡破坏(+/-UMMD)各半.全麻、MR平扫并静推Feridex 100μmol Fe/kg后,立即超声聚焦主动脉弓上缘前壁,缓慢静推Sonovue+生理盐水10 ml,MI1.2,3.5 MPa进行UMMD.3T磁共振仪,膝部线圈,主动脉弓上下120 mm范围,2 mm层厚.3D快速小角度激发GRE序列平扫及增强1、24、48、72 h及处死后扫描,标本送光、电镜检.重复测量资料方差分析检验各组数据.结果 UMMD对SPIO粒子进入血管壁差异有统计学意义(P=0.0004).超顺磁氧化铁(SPIO)粒子进入血管壁,以分散形式分布,间质居多,UMMD对内中外膜分布的影响具有明显作用(P=0.01),以外膜分布居多.光、电镜下可见微泡气体进入血管内中膜,油红O染证实.细胞内与细胞外铁粒子染色后表现不同.结论 UMMD使较大粒径SPIO粒子和气体进入血管壁.可能为大分子复合探针和携基因药物跨血管内膜提供新途径.
目的 探索血池內造影劑Sonovue和Feridex進入血管壁的可能性.方法 普通級雄性日本大耳白兔17隻,11隻高脂飼養動脈粥樣硬化(AS)模型,6隻普食正常對照.數字隨機取AS組10隻和正常組6隻,用和不用超聲介導微泡破壞(+/-UMMD)各半.全痳、MR平掃併靜推Feridex 100μmol Fe/kg後,立即超聲聚焦主動脈弓上緣前壁,緩慢靜推Sonovue+生理鹽水10 ml,MI1.2,3.5 MPa進行UMMD.3T磁共振儀,膝部線圈,主動脈弓上下120 mm範圍,2 mm層厚.3D快速小角度激髮GRE序列平掃及增彊1、24、48、72 h及處死後掃描,標本送光、電鏡檢.重複測量資料方差分析檢驗各組數據.結果 UMMD對SPIO粒子進入血管壁差異有統計學意義(P=0.0004).超順磁氧化鐵(SPIO)粒子進入血管壁,以分散形式分佈,間質居多,UMMD對內中外膜分佈的影響具有明顯作用(P=0.01),以外膜分佈居多.光、電鏡下可見微泡氣體進入血管內中膜,油紅O染證實.細胞內與細胞外鐵粒子染色後錶現不同.結論 UMMD使較大粒徑SPIO粒子和氣體進入血管壁.可能為大分子複閤探針和攜基因藥物跨血管內膜提供新途徑.
목적 탐색혈지내조영제Sonovue화Feridex진입혈관벽적가능성.방법 보통급웅성일본대이백토17지,11지고지사양동맥죽양경화(AS)모형,6지보식정상대조.수자수궤취AS조10지화정상조6지,용화불용초성개도미포파배(+/-UMMD)각반.전마、MR평소병정추Feridex 100μmol Fe/kg후,립즉초성취초주동맥궁상연전벽,완만정추Sonovue+생리염수10 ml,MI1.2,3.5 MPa진행UMMD.3T자공진의,슬부선권,주동맥궁상하120 mm범위,2 mm층후.3D쾌속소각도격발GRE서렬평소급증강1、24、48、72 h급처사후소묘,표본송광、전경검.중복측량자료방차분석검험각조수거.결과 UMMD대SPIO입자진입혈관벽차이유통계학의의(P=0.0004).초순자양화철(SPIO)입자진입혈관벽,이분산형식분포,간질거다,UMMD대내중외막분포적영향구유명현작용(P=0.01),이외막분포거다.광、전경하가견미포기체진입혈관내중막,유홍O염증실.세포내여세포외철입자염색후표현불동.결론 UMMD사교대립경SPIO입자화기체진입혈관벽.가능위대분자복합탐침화휴기인약물과혈관내막제공신도경.
Objective To explore feasibility for entrance of the contrast agent Sonovue and Feridex into the aortal wall.Methods 17 male Japanese giant ears rabbits (common grade),including 11 atherosclerosis (AS) animal models fed with food containing high-content lipid and normal animals fed with common food as control.Respectively,10 animals in the AS group and 6 animals in the normal group were selected in a random way to undergo ultrasound-mediated microbubble destruction (UMMD) and no ultrasound-mediated microbubble destruction (-UMMD) half and half.One animal was administrated with double doses of Feridex.After general anesthesia,MR plain scan and intravenous injection of Feridex 100 μmol Fe/kg,immediately ultrasound focused on the front wall of the aortic arch,which underwent UMMD at the pressure of 3.5 Mpa with MI1.2 while 10 ml solution (Sonovue+ normal saline) was injected intravenously at the speed of 0.5 ml/min FOR 20 min.3T magnetic resonance (MR) was performed with a moderately T2 * weighted gradient sequence.Enhanced scan were performed for 1 h,24 h,48 h,72 h and after killing the animal, then the specimen were delivered to conduct optical and electronic microscope examination.Variance test for the re-measured data was adopted to verify the data obtained in every group.Results The effect of UMMD group on SPIO particles entrance into the aortal wall is of marked significance (P=0.0004) statistically.The effect of UMMD on distribution in the vessel wall is of statistical significance (P =0.01),more particles in the dventitia.Gas or microbubbles were found to enter into the intima,media of the aorta,and verified by Oil Red O staining.After staining the findings of iron particle in the cell and out of the cell are different.Conclusions UMMD may facilitate entrance of those SPIO particles with a bigger diameter and microbublles into the aortal walL This discovery may provide a new solution for penetration of complex macromolecule probes and gene-carried drug through the tunica intima of the aorta.
