CAJ | 학술논문

目的探讨调节性T细胞(Tregs)在胸腺中的原位表达及其对重症肌无力(MG)发病的意义.方法选取未行免疫抑制治疗的MG患者胸腺39例,对照组9例.用间接免疫荧光双标记方法对胸腺Tregs进行定位分析,借助图像分析软件定量分析.结果 (1)MG组和正常对照组胸腺组织的Tregs均表达在胸腺髓质细胞区.(2)胸腺生发中心的细胞高表达CD25,但CD4表达较弱.表明生发中心多数为B细胞集合.(3)生发中心周围调节性T细胞分布较少.(4)平均视野阳性细胞绝对数MG组(17±3)与对照组(18±4)差异无统计学意义(P>0.05),平均视野阳性细胞比例对照组(45%±7%)高于MG组(36%±7%)(P<0.05).结论 MG患者胸腺组织存在Tregs数量比例的减少,因该种细胞比例的减少引发的免疫调节及抑制功能的下降可能是MG的促发因素之一.
목적 탐토조절성T세포(Tregs)재흉선중적원위표체급기대중증기무력(MG)발병적의의.방법 선취미행면역억제치료적MG환자흉선39례,대조조9례.용간접면역형광쌍표기방법대흉선Tregs진행정위분석,차조도상분석연건정량분석.결과 (1)MG조화정상대조조흉선조직적Tregs균표체재흉선수질세포구.(2)흉선생발중심적세포고표체CD25,단CD4표체교약.표명생발중심다수위B세포집합.(3)생발중심주위조절성T세포분포교소.(4)평균시야양성세포절대수MG조(17±3)여대조조(18±4)차이무통계학의의(P>0.05),평균시야양성세포비례대조조(45%±7%)고우MG조(36%±7%)(P<0.05).결론 MG환자흉선조직존재Tregs수량비례적감소,인해충세포비례적감소인발적면역조절급억제공능적하강가능시MG적촉발인소지일.
Objective To investigate the in situ expression of regulatory T cells (Tress) in thymus and its significance for myasthenia gravis (MG). Methods Thirty nine MG patients who had not accepted immunosuppressive therapy before thymectomy were recruited as study group and 19 patients undergoing eardiosurgery as control. There was no significant statistical difference in age and gender between the two groups (P> 0.05) . The location and proportion of Tress in thymus were analyzed using indirect immunofluorescence double labeling immunohistochemistry and image analysis software. Results (1) Tress were all located in medulla zone of thymus in control and MG groups. (2) Cells of germinal center had a high expression of CD25 and a low expression of CD4. It indicates that a majority of germinal center cells were B cells. (3) There were fewer Tress around germinal center. (4) There was no statistical significant difference in absolute number of Tregs between the two groups (P > 0.05). But the proportion of Tress was higher in control group (P < 0.05). Conclusion The proportion of thymus Tress decreases in MG so as to weaken the function of immuno-regulation and suppression. This is probably a precipitating factor of MG.