CAJ | 학술논문

目的应用18F-FDG和11C-NMSP microPET评估大鼠帕金森病(PD)模型脑局部葡萄糖代谢及多巴胺2型受体(DRD2)表达的差别及意义.方法采用6-羟多巴胺制备偏侧PD大鼠模型,2周后应用18F-FDG和11C-NMSPmicroPET扫描,比较PD模型组大鼠和对照组大鼠的脑局部葡萄糖代谢及DRD2表达,用免疫组化染色比较两组的酪氨酸羟化酶表达.结果 PD模型大鼠纹状体、海马、感觉运动皮质葡萄糖代谢率分别为88.2%±2.2%,94.5%±4.5%,96.2%±5.8%,显著低于对照组相应脑区92.7%±2.8%(P＜0.01),98.9%±3.9%(P＜0.01),102.8%±2.1%(P＜0.01);DRD2表达在PD模型的右侧纹状体为112.9%±9.0%,明显高于对照组102.3%±1.4%(P＜0.01).结论 PD模型大鼠损毁侧纹状体、海马、感觉运动皮质葡萄糖代谢均降低;而损毁侧纹状体DRD2表达明显升高.18F-FDG和11C-NMSP microPET能有效评估PD模型脑局部葡萄糖代谢及DRD2表达水平,可能成为早期诊断PD的分子影像学工具.
목적 응용18F-FDG화11C-NMSP microPET평고대서파금삼병(PD)모형뇌국부포도당대사급다파알2형수체(DRD2)표체적차별급의의.방법 채용6-간다파알제비편측PD대서모형,2주후응용18F-FDG화11C-NMSPmicroPET소묘,비교PD모형조대서화대조조대서적뇌국부포도당대사급DRD2표체,용면역조화염색비교량조적락안산간화매표체.결과 PD모형대서문상체、해마、감각운동피질포도당대사솔분별위88.2%±2.2%,94.5%±4.5%,96.2%±5.8%,현저저우대조조상응뇌구92.7%±2.8%(P＜0.01),98.9%±3.9%(P＜0.01),102.8%±2.1%(P＜0.01);DRD2표체재PD모형적우측문상체위112.9%±9.0%,명현고우대조조102.3%±1.4%(P＜0.01).결론 PD모형대서손훼측문상체、해마、감각운동피질포도당대사균강저;이손훼측문상체DRD2표체명현승고.18F-FDG화11C-NMSP microPET능유효평고PD모형뇌국부포도당대사급DRD2표체수평,가능성위조기진단PD적분자영상학공구.
Objective To employ 18F-fluoro-2-aeoxyglucose (18 F-FDG) and (3-N-[11 C] methylspiperone ) 11C -NMSP microPET to assess the changes of regional cerebral glucose metabolism and the expression of dopamine receptor type 2 ( DRD2 ) in a rat model of Parkinson's disease (PD). Methods A hemiparkinsonian model was established in rats by unilateral pretreatment with 6-hydroxydopamine (6-OHDA). At 2 weeks after 6-OHDA insult, 18F-FDG and 11C-NMSP microPET scan were performed to compare the differences of regional cerebral glucose metabolism and the expression of DRD2 between the PD and control groups respectively. The immunohistochemical staining was used to detect the expression of tyrosine hydroxylase in two groups. Results In the PD model, the glucose metabolism rates were 88. 2% ± 2. 2%, 94. 5% ±4.5% and 96. 2% ±5. 8% respectively, in right striatum, hippocampus and sensorimotor cortex. And they were significantly lower than those in the control group [ 92. 7% ± 2. 8% ( P ＜ 0. 01 ),98.9% ±3.9% (P＜0.01) & 102.8% ±2. 1% (P＜0.01) ].The expression of DRD2 in right striatum was significantly higher in the PD group than that in the control group ( 112. 9% ± 9. 0% vs 102. 3% ± 1.4%, P ＜ 0. 01 ). Conclusion In the PD rats, glucose metabolism decreases in injured side striatum,hippocampus and sensorimotor cortex while and the expression of DRD2 increases in injured side striatum.18F-FDG and 11C-NMSP microPET can effectively assess the regional cerebral glucose metabolism and the expression of DRD2 in PD. They may serve as effective molecular imaging tools for an early diagnosis of PD.