CAJ | 학술논문

目的建立一种多重PCR点突变筛查技术,用以细胞色素氧化酶P450 2C19(CYP2C19)的基因分型.方法设计含有拱桥式次黄嘌呤的多重PCR引物(DMP).在一个PCR管内,同时检测CYP2C19的*1、*2、*3等位基因.结果多重PCR点突变筛查技术,最低检测下限可以达到2.8 ng,具有较高的检测灵敏度.其成功检出 248份健康人抗凝静脉血CYP2C19等位基因*1、*2、*3.并与部分标本测序法结果比较,结果与测序法结果完全一致.结论成功建立了多重PCR点突变筛查技术,该技术特异性高、操作简单、价格低廉,为基础研究、个体化用药提供了合理可靠的检测手段.
목적 건립일충다중PCR점돌변사사기술,용이세포색소양화매P450 2C19(CYP2C19)적기인분형.방법 설계함유공교식차황표령적다중PCR인물(DMP).재일개PCR관내,동시검측CYP2C19적*1、*2、*3등위기인.결과 다중PCR점돌변사사기술,최저검측하한가이체도2.8 ng,구유교고적검측령민도.기성공검출 248빈건강인항응정맥혈CYP2C19등위기인*1、*2、*3.병여부분표본측서법결과비교,결과여측서법결과완전일치.결론 성공건립료다중PCR점돌변사사기술,해기술특이성고、조작간단、개격저렴,위기출연구、개체화용약제공료합리가고적검측수단.
Objective To establish a multiplex PCR point mutation screening technique for the genotyping of CYP2C19.Methods Deoxyinosine multiplex-polymerase chain reaction (PCR) primers (DMPs) were designed to detect simultaneously CYP2C19 * 1, * 2, * 3 alleles in one PCR tube.Results The above technique could detect the genotypes of CYP2C19 * 1, CYP2C19 * 2 and CYP2C19 * 3 successfully.And the results were completely consistent with those of DNA sequencing.Conclusion A novel screening technique of multiplex PCR point mutation is successfully established.With the advantages of high specificity, convenient handling, fast completion and low cost, it provides a reasonable and reliable detection method for basic researches and personalized medicine.