中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2011年
47期
3354-3357
,共4页
刘珍娟%王中秋%杨志坚%安晓静%李林%童明敏
劉珍娟%王中鞦%楊誌堅%安曉靜%李林%童明敏
류진연%왕중추%양지견%안효정%리림%동명민
胰腺肿瘤%磁共振成像%小鼠,裸%模型,动物
胰腺腫瘤%磁共振成像%小鼠,裸%模型,動物
이선종류%자공진성상%소서,라%모형,동물
Pancreatic neoplasms%Magnetic resonance imaging%Mice,nude%Models,animal
目的 探讨人胰腺癌裸鼠原位模型种植方法,研究其MRI表现及病理基础,为活体研究胰腺癌奠定基础.方法 采用人胰腺癌BXPC-3细胞对裸鼠进行原位种植,并使用3.0 T MRI成像,包括T1WI、T2WI及增强扫描,结合病理,分析人胰腺癌裸鼠原位模型的MRI表现.结果 18只裸鼠有15只接种成功,接种成功率为83.3％( 15/18).病理学检查符合胰腺高分化实体癌.对15只裸鼠行磁共振成像,肿瘤MR1表现为10只T1WI呈均匀稍低信号(66.7％),5只(33.3％)信号不均,以稍低信号为主；T2 WI呈均匀(26.7％,4/15)或不均匀(73.3％,11/15)稍高信号,增强扫描瘤灶边缘强化较明显,瘤灶轻度强化,坏死部分不强化.结论 3.0 T MRI能够检测出直径≥2 mm的胰腺肿瘤,并能够清晰显示裸鼠原位模型肿瘤的位置、形态、大小及其内部结构,能为胰腺癌进一步活体研究提供可视化平台.
目的 探討人胰腺癌裸鼠原位模型種植方法,研究其MRI錶現及病理基礎,為活體研究胰腺癌奠定基礎.方法 採用人胰腺癌BXPC-3細胞對裸鼠進行原位種植,併使用3.0 T MRI成像,包括T1WI、T2WI及增彊掃描,結閤病理,分析人胰腺癌裸鼠原位模型的MRI錶現.結果 18隻裸鼠有15隻接種成功,接種成功率為83.3％( 15/18).病理學檢查符閤胰腺高分化實體癌.對15隻裸鼠行磁共振成像,腫瘤MR1錶現為10隻T1WI呈均勻稍低信號(66.7％),5隻(33.3％)信號不均,以稍低信號為主；T2 WI呈均勻(26.7％,4/15)或不均勻(73.3％,11/15)稍高信號,增彊掃描瘤竈邊緣彊化較明顯,瘤竈輕度彊化,壞死部分不彊化.結論 3.0 T MRI能夠檢測齣直徑≥2 mm的胰腺腫瘤,併能夠清晰顯示裸鼠原位模型腫瘤的位置、形態、大小及其內部結構,能為胰腺癌進一步活體研究提供可視化平檯.
목적 탐토인이선암라서원위모형충식방법,연구기MRI표현급병리기출,위활체연구이선암전정기출.방법 채용인이선암BXPC-3세포대라서진행원위충식,병사용3.0 T MRI성상,포괄T1WI、T2WI급증강소묘,결합병리,분석인이선암라서원위모형적MRI표현.결과 18지라서유15지접충성공,접충성공솔위83.3％( 15/18).병이학검사부합이선고분화실체암.대15지라서행자공진성상,종류MR1표현위10지T1WI정균균초저신호(66.7％),5지(33.3％)신호불균,이초저신호위주；T2 WI정균균(26.7％,4/15)혹불균균(73.3％,11/15)초고신호,증강소묘류조변연강화교명현,류조경도강화,배사부분불강화.결론 3.0 T MRI능구검측출직경≥2 mm적이선종류,병능구청석현시라서원위모형종류적위치、형태、대소급기내부결구,능위이선암진일보활체연구제공가시화평태.
Objective To assess the methods of establishing an orthotopic nude murine model of human pancreatic cancer and explore the manifestations of MRI (magnetic resonance imaging) and its pathological fundamentals so as to provide research rationales for human pancreatic cancer.Methods The BXPC-3 cell of human pancreatic cancer was orthotopically planted in nude mice.And the animals were examined by a clinical 3.0 T magnetic resonance imager.The MRI examinations were analyzed along with their pathological findings.Results Among 18 mice,15 of them grew tumors with a success ratio of 83.3％.The pathological findings conformed to those of high differentiation pancreatic parenchyma cancer.Comparing with the neighbor muscles, the tumors were homogeneous (66.7％, 10/15 ) or heterogeneous (33.3％,5/15) of hypointense on Tl-weighted images while homogeneous (26.7％,4/15)or heterogeneous ( 73.3％, 11/15 ) hyperintense on T2-weighted images with heterogeneous enhancement.The border became obviously enhanced and there was mild central enhancement while the necrotic part showed no enhancement.Conclusion 3.0T MRI can detect pancreatic neoplasms ≥2 mm and visualize clearly their locations,shapes,dimensions and internal structures in an orthotopic nude murine model.Thus it provides a visible framework for further studies of human pancreatic cancer.
