中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2011年
48期
3438-3441
,共4页
江德鹏%李琪%维克多·克罗索夫%尤列·皮尔曼%周向东
江德鵬%李琪%維剋多·剋囉索伕%尤列·皮爾曼%週嚮東
강덕붕%리기%유극다·극라색부%우렬·피이만%주향동
肺疾病,慢性阻塞性%分泌型白细胞蛋白酶抑制因子%间质干细胞%动物实验
肺疾病,慢性阻塞性%分泌型白細胞蛋白酶抑製因子%間質榦細胞%動物實驗
폐질병,만성조새성%분비형백세포단백매억제인자%간질간세포%동물실험
Pulmonary disease,chronic obstructive%Secretary leukocyte protease inhibitor%Mesenchymal stem cells%Animal experimentation
目的 研究携分泌型白细胞蛋白酶抑制因子( SLPI)骨髓间充质干细胞(BMSCs)对慢性阻塞性肺疾病(COPD)大鼠气道炎症和黏液分泌的影响.方法 SD大鼠60只按随机数字表法随机分为阴性对照组、COPD模型组、BMSCs组和携SLPI质粒BMSCs组各15只,采用熏香烟加气管内注入内毒素法制备COPD模型,通过尾静脉注入BMSCs或携SLPI质粒BMSCs细胞.Western印迹检测SLPI表达,酶联免疫吸附法检测白细胞介素8(IL-8)和肿瘤坏死因子α(TNF-α)表达,通过病理切片观察杯状细胞增生情况.结果 携SLPI质粒BMSCs组支气管/肺组织中SLPI表达为(0.79±0.06),显著高于阴性对照组的(0.24±0.02),(P＜0.05).BMSCs组和携SLPI质粒BMSCs组支气管肺泡灌洗液中炎性因子IL-.8、TNF-α浓度均低于COPD模型组(均P＜0.05)；携SLPI质粒BMSCs组更低,分别为(17.6±1.7)和(36.6±4.0)ng/L,显著低于BMSCs组(均P＜0.05).携SLPI质粒BMSCs组支气管杯状细胞数较COPD模型组和BMSCs组明显减少(均P＜0.05).结论 携SLPI质粒BMSCs移植能够减轻COPD大鼠气道炎症和黏液分泌,有望用于对COPD患者的治疗.
目的 研究攜分泌型白細胞蛋白酶抑製因子( SLPI)骨髓間充質榦細胞(BMSCs)對慢性阻塞性肺疾病(COPD)大鼠氣道炎癥和黏液分泌的影響.方法 SD大鼠60隻按隨機數字錶法隨機分為陰性對照組、COPD模型組、BMSCs組和攜SLPI質粒BMSCs組各15隻,採用熏香煙加氣管內註入內毒素法製備COPD模型,通過尾靜脈註入BMSCs或攜SLPI質粒BMSCs細胞.Western印跡檢測SLPI錶達,酶聯免疫吸附法檢測白細胞介素8(IL-8)和腫瘤壞死因子α(TNF-α)錶達,通過病理切片觀察杯狀細胞增生情況.結果 攜SLPI質粒BMSCs組支氣管/肺組織中SLPI錶達為(0.79±0.06),顯著高于陰性對照組的(0.24±0.02),(P＜0.05).BMSCs組和攜SLPI質粒BMSCs組支氣管肺泡灌洗液中炎性因子IL-.8、TNF-α濃度均低于COPD模型組(均P＜0.05)；攜SLPI質粒BMSCs組更低,分彆為(17.6±1.7)和(36.6±4.0)ng/L,顯著低于BMSCs組(均P＜0.05).攜SLPI質粒BMSCs組支氣管杯狀細胞數較COPD模型組和BMSCs組明顯減少(均P＜0.05).結論 攜SLPI質粒BMSCs移植能夠減輕COPD大鼠氣道炎癥和黏液分泌,有望用于對COPD患者的治療.
목적 연구휴분비형백세포단백매억제인자( SLPI)골수간충질간세포(BMSCs)대만성조새성폐질병(COPD)대서기도염증화점액분비적영향.방법 SD대서60지안수궤수자표법수궤분위음성대조조、COPD모형조、BMSCs조화휴SLPI질립BMSCs조각15지,채용훈향연가기관내주입내독소법제비COPD모형,통과미정맥주입BMSCs혹휴SLPI질립BMSCs세포.Western인적검측SLPI표체,매련면역흡부법검측백세포개소8(IL-8)화종류배사인자α(TNF-α)표체,통과병리절편관찰배상세포증생정황.결과 휴SLPI질립BMSCs조지기관/폐조직중SLPI표체위(0.79±0.06),현저고우음성대조조적(0.24±0.02),(P＜0.05).BMSCs조화휴SLPI질립BMSCs조지기관폐포관세액중염성인자IL-.8、TNF-α농도균저우COPD모형조(균P＜0.05)；휴SLPI질립BMSCs조경저,분별위(17.6±1.7)화(36.6±4.0)ng/L,현저저우BMSCs조(균P＜0.05).휴SLPI질립BMSCs조지기관배상세포수교COPD모형조화BMSCs조명현감소(균P＜0.05).결론 휴SLPI질립BMSCs이식능구감경COPD대서기도염증화점액분비,유망용우대COPD환자적치료.
Objective To explore the effects of secretary leukocyte protease inhibitor (SLPI)-transfected bone marrow mesenchymal stem cells (BMSCs) transplantation on airway inflammation and mucus secretion in chronic obstructive pulmonary disease (COPD) rats.Methods Sixty rats were equally and randomly divided into negative contol,COPD model,BMSCs and SLPI-transfected BMSCs groups.The COPD rat model was established in all rats with the exception of the negative control rats by smoking and intratracheal instillation of lipopolysaccharide (LPS). BMSCs with or without transfection of plasmid containing SLPI gene were delivered through caudal vein of rats at 30 days post-induction.The expression of SLPI was examined with Western blot.The levels of interleukin (IL)-8 and tumor necrosis factor (TNF)-α were detected by enzyme-linked immunosorbent assay (ELISA).Goblet cell hyperplasia of lung pathological section was observed on. Results Compared with the negative control group,the expression of SLPI increased significantly after the administration of SLPI-transfected BMSCs (0.79 ± 0.06 vs 0.24 ± 0.02,P＜0.05).The levels of IL-8 and TNF-α in BMSCs and SLPI-transfected BMSCs group were lower than those in the COPD model group. Compared with the negative control group,the administration of SLPI-transfected BMSCs resulted in a further decrease in IL-8 and TNF-α in bronchoalveolar lavage fluid [ ( 17.6 ± 1.7 ) vs ( 36.6 ± 4.0 ) ng/L,P ＜ 0.05 ].SLPI-transfected BMSCs transplantation also significantly attenuated goblet cell hyperplasia in rats ( P ＜ 0.05 ).Conclusion There is a potential role for cell-based SLPI gene therapy in the treatment of COPD.
