CAJ | 학술논문

目的探讨西地那非对兔膀胱出口部分梗阻动物模型膀胱顺应性及膀胱组织内皮素1( ET-1)含量的影响.方法成年雄性新西兰白兔24只,随机数字表法分为4组,每组各6只.A组为假手术空白组:行假手术后每日生理盐水灌胃;B组为假手术治疗组:行假手术后每日西地那非(10 mg/kg)灌胃;C组为膀胱出口部分梗阻治疗组:行膀胱出口部分梗阻手术后每日西地那非(10 mg/kg)灌胃;D组为膀胱出口部分梗阻空白组:行膀胱出口部分梗阻手术后每日生理盐水灌胃.16周后4组分别行膀胱尿动力学检查,并取膀胱标本称重,采用酶联免疫法测定其ET-1含量.结果 A～D组膀胱排尿压力阈值分别为(10.6±2.0)、(11.6±2.7)、(14.0±4.2)、(20.4±6.1)cm H2O(1 cm H2O =0.098 kPa),D组明显高于A、B、C组(均P＜0.01);4组膀胱顺应性分别为(2.75±0.51)、(2.78 ±0.46)、(4.98±2.15)、(1.22±0.25) ml/cm H2O,D组明显低于A、B、C组(均P＜0.0l),C组高于A、B组(均P＜0.01),A组与B组间差异无统计学意义(P＞0.05);4组膀胱标本质量分别为(5.0±0.4)、(4.6±0.4)、(8.2±1.3)、(17.9±2.3)g,C、D组高于A、B组(均P＜0.01),D组高于C组(P＜0.01);4组膀胱组织中ET-1含量分别为(72±19)、(69 ± 18)、(76±21)、(106±29) pg/g,D组高于A、B、C组(均P＜0.05).结论每日西地那非能有效缓解兔膀胱出口部分梗阻对膀胱顺应性的损害,保护膀胱功能,并且此作用可能与降低梗阻后膀胱组织中ET-1浓度有关.
목적 탐토서지나비대토방광출구부분경조동물모형방광순응성급방광조직내피소1( ET-1)함량적영향.방법 성년웅성신서란백토24지,수궤수자표법분위4조,매조각6지.A조위가수술공백조:행가수술후매일생리염수관위;B조위가수술치료조:행가수술후매일서지나비(10 mg/kg)관위;C조위방광출구부분경조치료조:행방광출구부분경조수술후매일서지나비(10 mg/kg)관위;D조위방광출구부분경조공백조:행방광출구부분경조수술후매일생리염수관위.16주후4조분별행방광뇨동역학검사,병취방광표본칭중,채용매련면역법측정기ET-1함량.결과 A～D조방광배뇨압력역치분별위(10.6±2.0)、(11.6±2.7)、(14.0±4.2)、(20.4±6.1)cm H2O(1 cm H2O =0.098 kPa),D조명현고우A、B、C조(균P＜0.01);4조방광순응성분별위(2.75±0.51)、(2.78 ±0.46)、(4.98±2.15)、(1.22±0.25) ml/cm H2O,D조명현저우A、B、C조(균P＜0.0l),C조고우A、B조(균P＜0.01),A조여B조간차이무통계학의의(P＞0.05);4조방광표본질량분별위(5.0±0.4)、(4.6±0.4)、(8.2±1.3)、(17.9±2.3)g,C、D조고우A、B조(균P＜0.01),D조고우C조(P＜0.01);4조방광조직중ET-1함량분별위(72±19)、(69 ± 18)、(76±21)、(106±29) pg/g,D조고우A、B、C조(균P＜0.05).결론 매일서지나비능유효완해토방광출구부분경조대방광순응성적손해,보호방광공능,병차차작용가능여강저경조후방광조직중ET-1농도유관.
Objective To explore the effects of sildenafil on bladder compliance and endothelin-1 in the rabbit model of partial bladder outlet obstruction.Methods A total of 24 adult male New Zealand white rabbits were randomly divided into group A,group B,group C and group D (n =6 each).The rabbit model of partial bladder outlet obstruction was established in groups C and D while groups A and B underwent a sham operation.Daily sildenafil ( 10 mg/kg) was dosed to groups B and C by lavage.Daily normal saline was dosed similarly to groups A and D.Bladder urodynamic examinations were conducted in each group at Week 16.Then bladder was isolated and weighed from each group.And ET-1 in bladder tissue was measured by ELISA.Results Pressure thresholds for voiding (PT) in A-D groups were ( 10.6 ±2.0),(11.6 ±2.7),(14.0 ±4.2) and (20.4 ±6.1) cm H2O respectively.Compared with groups A,B and C,PT in group D was significantly higher ( all P ＜ 0.01 ).Bladder compliance in 4 groups were (2.75 ±0.51 ),(2.78 ± 0.46 ),( 4.98 ± 2.15 ) and ( 1.22 ± 0.25 ) ml/cm H2O respectively.Compared with groups A,B and C,bladder compliance was significantly lower in group D ( all P ＜ 0.01 ).Bladder compliance in group C was higher than that in groups A and B ( both P ＜ 0.01 ).The weights of bladder specimens in 4 groups were (5.0 ± 0.4),(4.6 ± 0.4),(8.2 ± 1.3 ) and ( 17.9 ± 2.3 ) g respectively.Compared with groups A and B,the weights of groups C and D were significantly heavier (all P ＜0.01 ).And the weight of group D was much greater than that of group C ( P ＜ 0.01 ).The contents of ET-1 in bladder tissue of 4 groups were (72 ± 19),(69 ± 18),(76 ± 21 ) and ( 106 ± 29) pg/g respectively.Compared with groups A,B and C,ET-1 in bladder tissue was significantly higher in group D (all P ＜0.05).Conclusions Daily sildenafil can effectively alleviate the damage of rabbit bladder compliance from partial bladder outlet obstruction and protect bladder functions.Its mechanism may be related with the downregulation of ET-1 in bladder tissue of partial bladder outlet obstruction.