CAJ | 학술논문

目的探讨腺病毒介导多巴胺2型受体短链亚型基因(D2S)联合溴隐亭药物干预对体外培养的原代人垂体无功能腺瘤(NFPA)细胞凋亡的影响.方法应用重组腺病毒载体pAd-D2S-EGFP将D2S基因导入原代贴壁培养的人NFPA细胞中,免疫荧光染色检测感染后细胞内D2S蛋白表达变化,转染后联合溴隐亭药物干预,并以CCK-8法检测加药后细胞生长活性,并用Hoechst染色细胞核凋亡形态学检测评估转染后药物干预对细胞活性的影响.结果 pAd-D2S-EGFP能够成功感染体外贴壁培养的原代人NFPA细胞,转染D2S-EGFP联合溴隐亭药物干预能够抑制NFPA细胞生长,溴隐亭干预后第48小时时,实验组细胞活性为(40±5)％,与单纯加药组(97±5)％及空载体组(90±9)％相比,差异有统计学意义(P＜0.05).实验组Hoechst染色观察到明显的凋亡现象.结论提高体外培养NFPA细胞的D2S表达会增加肿瘤细胞对溴隐亭治疗的敏感性.
목적 탐토선병독개도다파알2형수체단련아형기인(D2S)연합추은정약물간예대체외배양적원대인수체무공능선류(NFPA)세포조망적영향.방법 응용중조선병독재체pAd-D2S-EGFP장D2S기인도입원대첩벽배양적인NFPA세포중,면역형광염색검측감염후세포내D2S단백표체변화,전염후연합추은정약물간예,병이CCK-8법검측가약후세포생장활성,병용Hoechst염색세포핵조망형태학검측평고전염후약물간예대세포활성적영향.결과 pAd-D2S-EGFP능구성공감염체외첩벽배양적원대인NFPA세포,전염D2S-EGFP연합추은정약물간예능구억제NFPA세포생장,추은정간예후제48소시시,실험조세포활성위(40±5)％,여단순가약조(97±5)％급공재체조(90±9)％상비,차이유통계학의의(P＜0.05).실험조Hoechst염색관찰도명현적조망현상.결론 제고체외배양NFPA세포적D2S표체회증가종류세포대추은정치료적민감성.
Objective To explore the inhibitory effect of non-functioning pituitar adenoma (NFPA)cells after a combined treatment of adenovirus mediated D2S gene and bromocriptine in vitro.Methods Adenovirus containing dopamine 2 receptor short isoform (D2S) gene was used to infect NFPA cells.The transfection of D2S gene into NFPA cells was confirmed by immunofluorescence.And cell apoptosis of infected cells treated by bromocriptine was evaluated with CCK-8 assay in vitro.Results When D2S gene transfection and bromocriptine was used in combination,the survival rate of NFPA cells significantly decreased (40 ± 5)％ versus the control group (97 ± 5)％ and the pAd-EGFP transfection combined bromocriptine treatment group (90 ± 9) ％ (P ＜ 0.05).Conclusion The combined treatment of adenovirus-mediated D2S gene and bromocriptine can effectively induce the apoptosis of NFPA cells on primary culture and increase the sensitivity of NFPA to dopamine agonist.