中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
39期
2775-2777
,共3页
俞大亮%厉小梅%王喜梅%王晓秋%李向培
俞大亮%厲小梅%王喜梅%王曉鞦%李嚮培
유대량%려소매%왕희매%왕효추%리향배
干燥综合征%唇%外分泌腺
榦燥綜閤徵%脣%外分泌腺
간조종합정%진%외분비선
Sjogren's syndrome%Lip%Exocrineglands
目的 检测共刺激分子B7-H4在原发性干燥综合征(pSS)患者唇腺及血清中的表达及临床意义.方法 选择2009年6月至2011年1月安徽医科大学附属省立医院风湿免疫科门诊和住院确诊的pSS患者40例,免疫组织化学染色和流式细胞术检测唇腺组织中B7-H4表达；酶联免疫吸附法检测pSS患者和健康对照血清中可溶性B7-H4的含量.结果 免疫组织化学染色显示B7-H4表达在唇腺导管上皮细胞,pSS患者唇腺中B7-H4阳性导管数百分比为(42±21)％,疾病对照为(85±13)％,两者比较P ＜0.05.流式细胞术表明pSS患者唇腺单细胞悬液中B7-H4的表达为(18±14)％,对照为(48±22)％,两者比较P＜0.01.另外,pSS患者血清可溶性B7-H4的含量(49±31) μg/L,比正常健康人群的含量(71 ±27) μg/L明显减少(P＜0.01),并且其和唾液流率、泪液流率明显正相关(P＜0.01).结论 B7-H4共刺激分子在pSS患者的唇腺组织及血清表达减少,提示其可能参与了pSS的疾病进展,但其具体机制尚需要进一步研究.
目的 檢測共刺激分子B7-H4在原髮性榦燥綜閤徵(pSS)患者脣腺及血清中的錶達及臨床意義.方法 選擇2009年6月至2011年1月安徽醫科大學附屬省立醫院風濕免疫科門診和住院確診的pSS患者40例,免疫組織化學染色和流式細胞術檢測脣腺組織中B7-H4錶達；酶聯免疫吸附法檢測pSS患者和健康對照血清中可溶性B7-H4的含量.結果 免疫組織化學染色顯示B7-H4錶達在脣腺導管上皮細胞,pSS患者脣腺中B7-H4暘性導管數百分比為(42±21)％,疾病對照為(85±13)％,兩者比較P ＜0.05.流式細胞術錶明pSS患者脣腺單細胞懸液中B7-H4的錶達為(18±14)％,對照為(48±22)％,兩者比較P＜0.01.另外,pSS患者血清可溶性B7-H4的含量(49±31) μg/L,比正常健康人群的含量(71 ±27) μg/L明顯減少(P＜0.01),併且其和唾液流率、淚液流率明顯正相關(P＜0.01).結論 B7-H4共刺激分子在pSS患者的脣腺組織及血清錶達減少,提示其可能參與瞭pSS的疾病進展,但其具體機製尚需要進一步研究.
목적 검측공자격분자B7-H4재원발성간조종합정(pSS)환자진선급혈청중적표체급림상의의.방법 선택2009년6월지2011년1월안휘의과대학부속성립의원풍습면역과문진화주원학진적pSS환자40례,면역조직화학염색화류식세포술검측진선조직중B7-H4표체；매련면역흡부법검측pSS환자화건강대조혈청중가용성B7-H4적함량.결과 면역조직화학염색현시B7-H4표체재진선도관상피세포,pSS환자진선중B7-H4양성도관수백분비위(42±21)％,질병대조위(85±13)％,량자비교P ＜0.05.류식세포술표명pSS환자진선단세포현액중B7-H4적표체위(18±14)％,대조위(48±22)％,량자비교P＜0.01.령외,pSS환자혈청가용성B7-H4적함량(49±31) μg/L,비정상건강인군적함량(71 ±27) μg/L명현감소(P＜0.01),병차기화타액류솔、루액류솔명현정상관(P＜0.01).결론 B7-H4공자격분자재pSS환자적진선조직급혈청표체감소,제시기가능삼여료pSS적질병진전,단기구체궤제상수요진일보연구.
Objective To detect the expression of B7-H4 in salivary gland and sera in paticnts with primary Sjogren's syndrome (pSS).Methods A total of 40 pSS patients were referred to our department from June 2009 to January 2011.Immunohistochemistry and flow cytometry were used to detect the expression of B7-H4 in salivary gland from pSS patients and disease controls.Enzyme-linked immunosorbent assay (ELISA) was used to detect soluble B7-H4 of serum from pSS patients and healthy donors.Results Immunohistological staining revealed that B7-H4 antigen was restricted to tubular epithelium.The B7-H4 positive expression of tubules in salivary gland biopsies from pSS patients (18 ± 14)％ were lower than that of controls (85 ± 13)％ (P ＜ 0.05).Flow cytometry revealed that the B7-H4 expression of cell suspensions from salivary gland from pSS patients (42 ± 21)％ were lower than that of controls (48 ± 22)％(P ＜0.01).And the serum level of soluble B7-H4 detected in pSS patients (49 ± 31)μg/L significantly decreased than that in healthy donors (71 ± 27)μg/L (P ＜ 0.05)and positively correlated with saliva and tear flow rates (P ＜ 0.01) respectively.Conclusion The expression of B7-H4 molecule may play some roles in the progression of pSS.And a further understanding of its mechanism helps to elucidate the pathogenesis of pSS.
