CAJ | 학술논문

目的观察电刺激坐骨神经对新生期大鼠脊髓背角浅层、海马CA1区突触可塑性及c-fos蛋白表达的影响.方法 (1)将大鼠分为新生组及成年期组,记录左侧胸13～腰1脊髓背角浅层及右侧海马CA1区诱发场电位,予条件电刺激后,记录电位幅度,潜伏期变化至120 min,再在脊髓表面加D-AP5,继续观察场电位的幅度及潜伏期变化至175 min.(2)大鼠分为新生组、成年期组,每组数字随机法随机分为3组:空白对照、单刺激、条件电刺激后组,其中,条件电刺激后组分为条件电刺激后60、120和175 min组,实验后动物脊髓和海马做免疫组织化学染色,观察c-fos蛋白表达.结果 (1)电刺激坐骨神经在新生期组大鼠脊髓背角浅层及海马CA1区诱发产生NMDA受体依赖的长时程增强(LTP),主要由脊髓A类神经纤维诱发；成年期组大鼠以C类神经纤维诱发产生LTP.(2)条件电刺激后,在新生期大鼠脊髓(L1组由T0的10±8上升至T3的45±14；L2组由T0的13±10上升至T3的41±10；L3组由T0的19±7上升至T3的41±9)及海马CA1区c-fos蛋白(由T0的25±7上升为49 ±8)表达都增加,成年期大鼠脊髓背角浅层海马CA1区c-fos蛋白表达增加.结论条件电刺激在新生期组大鼠脊髓背角诱发A类神经纤维特征,NMDA受体依赖的LTP,同时c-fos蛋白在脊髓不同层和海马表达,说明痛觉信号随发育而变化.
목적 관찰전자격좌골신경대신생기대서척수배각천층、해마CA1구돌촉가소성급c-fos단백표체적영향.방법 (1)장대서분위신생조급성년기조,기록좌측흉13～요1척수배각천층급우측해마CA1구유발장전위,여조건전자격후,기록전위폭도,잠복기변화지120 min,재재척수표면가D-AP5,계속관찰장전위적폭도급잠복기변화지175 min.(2)대서분위신생조、성년기조,매조수자수궤법수궤분위3조:공백대조、단자격、조건전자격후조,기중,조건전자격후조분위조건전자격후60、120화175 min조,실험후동물척수화해마주면역조직화학염색,관찰c-fos단백표체.결과 (1)전자격좌골신경재신생기조대서척수배각천층급해마CA1구유발산생NMDA수체의뢰적장시정증강(LTP),주요유척수A류신경섬유유발；성년기조대서이C류신경섬유유발산생LTP.(2)조건전자격후,재신생기대서척수(L1조유T0적10±8상승지T3적45±14；L2조유T0적13±10상승지T3적41±10；L3조유T0적19±7상승지T3적41±9)급해마CA1구c-fos단백(유T0적25±7상승위49 ±8)표체도증가,성년기대서척수배각천층해마CA1구c-fos단백표체증가.결론 조건전자격재신생기조대서척수배각유발A류신경섬유특정,NMDA수체의뢰적LTP,동시c-fos단백재척수불동층화해마표체,설명통각신호수발육이변화.
Objective To explore the response to nociceptive stimuli in spinal cord of neonatal rat and observe the electrical stimulation of sciatic nerve on synaptic plasticity of spinal dorsal horn,hippocampal CA1 region and spinal c-fos,hippocampal CA1 region expression in neonatal rats.Methods The rats were divided into neonatal and adult groups.The evoked potentials of left spinal dorsal horn from T13-L1 and right hippocampal CA1 region were recorded.After conditional electric stimulations,the potential amplitudes were recorded.When the incubation period reached 120 min,D-AP5 was added to spinal cord of rats and resulting changes in field potential and c-fos expression were recorded.Results Long-term potentiation (LTP) in neonatal rats was mainly evoked by A-type nerve fibers whereas LTP in adult rats was mainly evoked by C-type nerve fibers.C-fos expression was significantly increased in superficial,deep layers of spinal dorsal horn,ventral horn and hippocampal CA1 region in neonatal rats.Conclusion Pain signals change with age.