CAJ | 학술논문

目的探讨脂多糖诱导的髓源抑制性细胞对哮喘小鼠气道炎症的影响及其机制.方法雌性BALB/c小鼠34只,其中4只接受脂多糖注射以诱导髓源抑制性细胞募集,采用CD11b磁珠从脾脏中分选该群细胞,其余30只随机数字表法分为对照组、哮喘组和治疗组.哮喘组和治疗组给予卵白蛋白致敏并雾化吸入建立哮喘模型.治疗组在诱导哮喘第14天及第21天分别注入体外磁珠分离的髓源抑制性细胞.各组在末次激发24h内计数支气管肺泡灌洗液(BALF)中细胞总数并分类,结合病理切片分析气道炎症情况 ;采用酶联免疫吸附(ELISA)法检测BALF和血清中白细胞介素13 (IL-13)的变化 ;流式细胞仪检测外周血CD4+ CD25+ Foxp3+调节性T细胞(简称Treg细胞)的比例.结果治疗组BALF中细胞总数、中性粒细胞、嗜酸粒细胞百分比[(17.0 ±8.3) ×104/ml、11.1％±2.0％、9.8％±2.9％]显著低于哮喘组[(36.0±15.9)×104/ml、20.8％±4.0％、14.1％±4.2％] (P =0.000、0.000、0.011),BALF和血清中IL-13含量[(34.7±7.1)和(34.0±4.7) ng/L]显著低于哮喘组[(105.0±9.0)和(48.1 ±6.1) ng/L](均P=0.000),外周血Treg细胞的比例(8.0％±1.3％)高于哮喘组(5.1％±2.1％)(P=0.002) ;治疗组气道炎症较哮喘组轻.结论脂多糖来源的髓源抑制性细胞可能通过抑制Th2过度免疫应答和上调小鼠外周血Treg细胞比例,从而减轻哮喘小鼠气道炎症.
목적 탐토지다당유도적수원억제성세포대효천소서기도염증적영향급기궤제.방법 자성BALB/c소서34지,기중4지접수지다당주사이유도수원억제성세포모집,채용CD11b자주종비장중분선해군세포,기여30지수궤수자표법분위대조조、효천조화치료조.효천조화치료조급여란백단백치민병무화흡입건립효천모형.치료조재유도효천제14천급제21천분별주입체외자주분리적수원억제성세포.각조재말차격발24h내계수지기관폐포관세액(BALF)중세포총수병분류,결합병리절편분석기도염증정황 ;채용매련면역흡부(ELISA)법검측BALF화혈청중백세포개소13 (IL-13)적변화 ;류식세포의검측외주혈CD4+ CD25+ Foxp3+조절성T세포(간칭Treg세포)적비례.결과 치료조BALF중세포총수、중성립세포、기산립세포백분비[(17.0 ±8.3) ×104/ml、11.1％±2.0％、9.8％±2.9％]현저저우효천조[(36.0±15.9)×104/ml、20.8％±4.0％、14.1％±4.2％] (P =0.000、0.000、0.011),BALF화혈청중IL-13함량[(34.7±7.1)화(34.0±4.7) ng/L]현저저우효천조[(105.0±9.0)화(48.1 ±6.1) ng/L](균P=0.000),외주혈Treg세포적비례(8.0％±1.3％)고우효천조(5.1％±2.1％)(P=0.002) ;치료조기도염증교효천조경.결론 지다당래원적수원억제성세포가능통과억제Th2과도면역응답화상조소서외주혈Treg세포비례,종이감경효천소서기도염증.
Objective To explore the effect and mechanism of lipopolysaccharides (LPS)-induced CD11b+Gr-1 + myeloid-derived suppressor cells (MDSCs) on airway inflammation in asthmatic mice.Methods A total of 34 female BALB/c mice were selected.Among them,4 mice received an intraperitoneal injection of LPS for inducing the accumulation of MDSCs.And the MDSCs were separated with CD11b immunomagnetic beads from spleen extract.Another 30 mice were randomly divided into normal control,asthmatic and cell treatment groups.The mice in the asthmatic and cell treatment groups were sensitized with ovalbumin by a combination of intraperitoneal injection and challenges to establish the murine asthmatic model.At Days 14 and 21 post-sensitization,the mice in cell treatment group received an intravenous injection of LPS-induced MDSCs.At 24 hours after the last allergen challenge,the number of inflammatory cells were counted and morphological identification of leucocytes in bronchoalveolar lavage fluid (BALF) was performed to analyze the degree of airway inflammation in conjunctions with pathological sections.The BALF and serum levels of interleukin-13 were measured by enzyme-linked immunosorbnent assay (ELISA).The number of CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) in peripheral blood was measured by flow cytometry.Results The total number of cells,the percentage of neutrophils and eosinophils of BALF in the cell treatment group [(17.0 ± 8.3) × 104/ml,11.1％ ± 2.0％,9.8％ ±2.9％] were significantly lower than those in the asthmatic group [(36.0 ± 15.9) × 104/ml,20.8％ ±4.0％,14.1％ ±4.2％] (P=0.000,0.000,0.011).Compared to the asthmatic group,the BALF and serum levels of IL-13 were significantly lower [(34.7 ±7.1) vs (105.0 ±9.0) ng/L,(34.0 ±4.7) vs(48.1 ± 6.1) ng/L] (both P =0.000) and the number of CD4 + CD25 + Foxp3 + regulatory T cells increased in peripheral blood (8.0％ ± 1.3％ vs 5.1％ ± 2.1％,P =0.002) and airway inflammation was significantly relieved in the cell treatment group.Conclusion LPS-induced MDSCs may improve airway inflammation through up-regulating Tregs in peripheral blood and suppressing Th2 effector function in asthmatic mice.