CAJ | 학술논문

目的通过检测MMP-9、PR、Ki-67和Survivin在多发性脑膜瘤组织中的表达情况,探讨多发性脑膜瘤的发生、诊断及生物学特性.方法收集山东大学齐鲁医院1992至2005年手术治疗的多发性脑膜瘤36例,与30例单发性脑膜瘤进行对比研究,所有标本进行诊断、组织学分型和分级,以正常硬脑膜组织作对照,应用免疫组织化学技术检测MMP-9、PR、Ki-67、Survivin在脑膜瘤中的表达,并进行定量分析.结果 MMP-9、PR、Ki-67、Survivin在正常硬脑膜组织中均未检测到,而在脑膜瘤表达率分别为100％(66/66)、53％(35/66)、23％(15/66)、88％(58/66),与正常组织比较差异有统计学意义,但多发性和单发性脑膜瘤中的表达阳性率差异无统计学意义.MMP-9、PR在两组的阳性表达强度差异有统计学意义,MMP-9在多发组中表达强度明显高于单发组(P＜0.01),而PR在多发组中表达强度明显低于单发组(P＜0.05),Ki-67、Survivin在两组中的阳性表达强度差异无统计学意义.结论 MMP-9、PR、Ki-67、Survivin检测有助于脑膜瘤的早期临床诊断.
목적 통과검측MMP-9、PR、Ki-67화Survivin재다발성뇌막류조직중적표체정황,탐토다발성뇌막류적발생、진단급생물학특성.방법 수집산동대학제로의원1992지2005년수술치료적다발성뇌막류36례,여30례단발성뇌막류진행대비연구,소유표본진행진단、조직학분형화분급,이정상경뇌막조직작대조,응용면역조직화학기술검측MMP-9、PR、Ki-67、Survivin재뇌막류중적표체,병진행정량분석.결과 MMP-9、PR、Ki-67、Survivin재정상경뇌막조직중균미검측도,이재뇌막류표체솔분별위100％(66/66)、53％(35/66)、23％(15/66)、88％(58/66),여정상조직비교차이유통계학의의,단다발성화단발성뇌막류중적표체양성솔차이무통계학의의.MMP-9、PR재량조적양성표체강도차이유통계학의의,MMP-9재다발조중표체강도명현고우단발조(P＜0.01),이PR재다발조중표체강도명현저우단발조(P＜0.05),Ki-67、Survivin재량조중적양성표체강도차이무통계학의의.결론 MMP-9、PR、Ki-67、Survivin검측유조우뇌막류적조기림상진단.
Objective To detect the expressions of matrix metalloproteinase-9(MMP-9),progesterone receptor(PR),Ki-67 and Survivin in the multiple meningiomas(MMs)and explore its genesis,diagnosis and bionomics features.Methods A total of 66 cases with histological sections of meningiomas retrieved from the archives of Department of Pathology of our hospital,including 30 cases of solitary meningiomas(SMs)and 36 cases MMs,were regrouped as benign,atypical and malignant by hematoxylin and eosin staining according to the World Health Organization classification of nervous system tumors.Immunohistochemistry was performed to detect the expressions of MMP-9,PR,Ki-67 and Survivin in 36 cases of MMs and 30 cases of SMs.And normal brain tissue was selected as a control group.The staining intensity was analyzed quantitatively for the differential expressions of MMP-9,PR,Ki-67 and Survivin between SMs and MMs.Results No expression of MMP-9,PR,Ki-67 and Survivin was detected in 5 normal brain tissues,but the expression rates were 100％,53％,23％ and 88％ respectively for significant difference comparing with normal tissue.The result of statistical analysis showed that there was significant difference in the expression intensity of MMP-9 and PR between two groups.The expression intensity MMP-9 in multiple group was significantly higher in MMs than that in SMs(P ＜ 0.01)while PR was lower in MMs than that in SMs(P ＜ 0.05).But no significant difference was found for the expression of Ki-67 or Survivin between two groups.Conclusion The detections of MMP-9,PR,Ki-67 and Survivin are helpful in the clinical diagnosis and early detection of meningioma.