CAJ | 학술논문

目的了解低氧诱导因子1a(HIF-1a)在急性大鼠下肢缺血肌组织中表达的变化规律.方法以SD成年大鼠建立下肢缺血模型,根据阻断左骼总动脉的时间分别设假手术组(0h组)、4h组、8h组、12 h组和24h组,切取下肢腓肠肌,通过免疫组织化学、免疫印迹(Western印迹)及实时定量逆转录酶聚合酶链反应(RT-PCR)检测缺血腓肠肌中HIF-1a的表达.结果 HIF-1a在大鼠正常腓肠肌中呈低水平表达,而在各缺血组中的表达均明显高于假手术组(P＜0.05),并且在缺血早中期(4h和8h),其表达达到高峰,随着缺血时间的延长,HIF-1a表达呈下降趋势.结论 HIF-1a在大鼠急性下肢缺血过程中存在特征性的变化趋势,推测HIF-1a可能是急性缺血过程中一种重要的适应性调节.
목적 료해저양유도인자1a(HIF-1a)재급성대서하지결혈기조직중표체적변화규률.방법 이SD성년대서건립하지결혈모형,근거조단좌격총동맥적시간분별설가수술조(0h조)、4h조、8h조、12 h조화24h조,절취하지비장기,통과면역조직화학、면역인적(Western인적)급실시정량역전록매취합매련반응(RT-PCR)검측결혈비장기중HIF-1a적표체.결과 HIF-1a재대서정상비장기중정저수평표체,이재각결혈조중적표체균명현고우가수술조(P＜0.05),병차재결혈조중기(4h화8h),기표체체도고봉,수착결혈시간적연장,HIF-1a표체정하강추세.결론 HIF-1a재대서급성하지결혈과정중존재특정성적변화추세,추측HIF-1a가능시급성결혈과정중일충중요적괄응성조절.
Objective To explore the expression of hypoxia-inducible factor 1 alpha(HIF-1a)in rat lower limb skeletal muscle with acute ischemia.Methods Acute lower limb ischemia was established in 25 male or female SD rats of 200-250 g by ligating left iliac artery with microsurgical techniques.Gastrocnemius of lower limb was harvest at the timepoints of sham(0h),4,8,12 and 24 h respectively.Immunohistochemistry,Western blot and real-time polymerase chain reaction(PCR)were used to detect the expression of HIF-1a in lower limb gastrocnemius.Results There was a low expression of HIF-1a in normal gastrocnemius,but it was highly expressed in lower limb ischemia.With the elongation of ischemia time,its expression increased(P ＜ 0.05),peaked at 4 h and 8 h(P ＜ 0.05)and then declined gradually.Conclusion With a characteristic trend during acute limb ischemia,HIF-1 a may play an important role in the adaptive response to acute limb ischemia.