中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
46期
3274-3278
,共5页
徐卫国%王宇鹏%郭志义%陈源水%董洪超%张鹏东%刘云%赵跃鹏%孙红%刘和亮
徐衛國%王宇鵬%郭誌義%陳源水%董洪超%張鵬東%劉雲%趙躍鵬%孫紅%劉和亮
서위국%왕우붕%곽지의%진원수%동홍초%장붕동%류운%조약붕%손홍%류화량
结直肠肿瘤%神经纤毛蛋白质2%微淋巴管密度
結直腸腫瘤%神經纖毛蛋白質2%微淋巴管密度
결직장종류%신경섬모단백질2%미림파관밀도
Colorectal cancer%Neuropilin-2%Microlymphatic density
目的 探讨大肠癌组织中神经纤毛蛋白(NRP)-2的表达及其与肿瘤淋巴管生成和淋巴道转移的相关性.方法 系统随机选取河北联合大学附属医院2010年3月至2012年1月手术切除的55例大肠癌患者的大肠癌组织、癌旁组织及相应的正常组织标本,病理结果均经病理组织学证实.采用反转录(RT)-PCR法、免疫组化法检测大肠癌组织、癌旁组织中NRP-2的表达情况,并通过D2-40单克隆抗体特异性标记的肿瘤新生淋巴管,计数微淋巴管密度(MLD)值.结果 大肠癌组织、癌旁组织中新生淋巴管的数目存在差异,MLD值分别为(39±19)、(53±26)个,差异有统计学意义(P＜0.01),新生淋巴管的形态亦存在差异.蛋白水平上,大肠癌组织中NRP-2的表达与MLD值呈正相关(r=0.325,P＜0.05)；基因水平上,大肠癌组织中NRP-2的表达与MLD值呈正相关(r=0.545,P=0.000).NRP-2 mRNA的表达与大肠癌的分化程度、浸润深度、淋巴管浸润、淋巴结转移、Dukes分期相关(均P＜0.05).结论 NRP-2的表达可能调节大肠癌局部淋巴管的生成,并在大肠癌的发生和发展过程中可能发挥重要作用.
目的 探討大腸癌組織中神經纖毛蛋白(NRP)-2的錶達及其與腫瘤淋巴管生成和淋巴道轉移的相關性.方法 繫統隨機選取河北聯閤大學附屬醫院2010年3月至2012年1月手術切除的55例大腸癌患者的大腸癌組織、癌徬組織及相應的正常組織標本,病理結果均經病理組織學證實.採用反轉錄(RT)-PCR法、免疫組化法檢測大腸癌組織、癌徬組織中NRP-2的錶達情況,併通過D2-40單剋隆抗體特異性標記的腫瘤新生淋巴管,計數微淋巴管密度(MLD)值.結果 大腸癌組織、癌徬組織中新生淋巴管的數目存在差異,MLD值分彆為(39±19)、(53±26)箇,差異有統計學意義(P＜0.01),新生淋巴管的形態亦存在差異.蛋白水平上,大腸癌組織中NRP-2的錶達與MLD值呈正相關(r=0.325,P＜0.05)；基因水平上,大腸癌組織中NRP-2的錶達與MLD值呈正相關(r=0.545,P=0.000).NRP-2 mRNA的錶達與大腸癌的分化程度、浸潤深度、淋巴管浸潤、淋巴結轉移、Dukes分期相關(均P＜0.05).結論 NRP-2的錶達可能調節大腸癌跼部淋巴管的生成,併在大腸癌的髮生和髮展過程中可能髮揮重要作用.
목적 탐토대장암조직중신경섬모단백(NRP)-2적표체급기여종류림파관생성화림파도전이적상관성.방법 계통수궤선취하북연합대학부속의원2010년3월지2012년1월수술절제적55례대장암환자적대장암조직、암방조직급상응적정상조직표본,병리결과균경병리조직학증실.채용반전록(RT)-PCR법、면역조화법검측대장암조직、암방조직중NRP-2적표체정황,병통과D2-40단극륭항체특이성표기적종류신생림파관,계수미림파관밀도(MLD)치.결과 대장암조직、암방조직중신생림파관적수목존재차이,MLD치분별위(39±19)、(53±26)개,차이유통계학의의(P＜0.01),신생림파관적형태역존재차이.단백수평상,대장암조직중NRP-2적표체여MLD치정정상관(r=0.325,P＜0.05)；기인수평상,대장암조직중NRP-2적표체여MLD치정정상관(r=0.545,P=0.000).NRP-2 mRNA적표체여대장암적분화정도、침윤심도、림파관침윤、림파결전이、Dukes분기상관(균P＜0.05).결론 NRP-2적표체가능조절대장암국부림파관적생성,병재대장암적발생화발전과정중가능발휘중요작용.
Objective To explore the relationship between neuropilin 2 (NRP-2) and lymphangiogenesis and lymphatic metastasis of human colorectal carcinoma (CRC),as well as the expression of NRP-2 in CRC tissues.Methods A total of 55 cases of CRC,adjacent and normal tissues of surgical resection were randomly selected at our hospital from March 2010 to January 2012.All pathological findings were confirmed by histopathology.The expression of NRP-2 was detected with reverse transcription (RT)-PCR and immunohistochemistry in parenchymatous and surrounding malignant tissues.Then lymphangiogenesis was marked with D2-40 monoclonal antibody and microlymphatic density (MLD) counted.Results Significant differences of MLD existed between those of tumor region(39 ± 19) and tumor margin(53 ± 26,P ＜0.01).Both the number and shape of lymphangiogenesis were different between the parenchymatous and surrounding tissues.The expression of NRP-2 had a positive correlation with MLD both at the protein level(r =0.325,P ＜ 0.05)and at the gene level (r =0.545,P =0.000).And it was also correlated with the differentiation degree,infiltrative degree,lymphovascular invasion,lymph node metastasis and Dukes tumor staging (all P ＜ 0.05).Conclusion The expression of NRP-2 may regulate lymphangiogenesis and it may play an important role in the incidence and development of CRC.
