中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
48期
3423-3425
,共3页
血小板计数%流式细胞术%未成熟血小板
血小闆計數%流式細胞術%未成熟血小闆
혈소판계수%류식세포술%미성숙혈소판
Platelet count%Flow cytometry%Immature platelets
目的 分析比较检测未成熟血小板的方法学差异,并调查本地区正常参考区间,为实验室开展未成熟血小板检测提供依据.方法 分析使用聚甲基(噁)嗪(PO)染色的全自动血细胞分析仪和用噻唑橙(TO)染色的传统流式细胞分析仪检测未成熟血小板的批内精密度、携带污染率等指标,分析同一仪器在不同时间检测的未成熟血小板百分比的差异,比较两种方法的性能,检测健康体检人群(n =336)的未成熟血小板百分比,分析两种方法的相关性,统计全自动血细胞分析仪的参考区间.结果 全自动血细胞分析仪的变异系数(CV)为16.2％、携带污染率为4.6％；流式细胞分析仪的最少CV为35.1％、携带污染率为4.8％.样本放置30 min、2h、4h后,全自动血细胞分析仪检测未成熟血小板,差异无统计学意义(P ＞0.05);TO染色后30 min、1h、2h用传统流式细胞分析仪检测的未成熟血小板,差异有统计学意义(P＜0.01).两种方法检测结果有相关性(r2=0.923,P＜0.01).全自动血细胞分析仪检测值的正常参考区间为1.0％～7.5％；流式细胞分析仪检测值的正常参考区间为3.0％～10.5％.结论 全自动血细胞分析仪法比传统流式细胞分析仪法操作简单,结果稳定性好,是检测未成熟血小板的优选方法.
目的 分析比較檢測未成熟血小闆的方法學差異,併調查本地區正常參攷區間,為實驗室開展未成熟血小闆檢測提供依據.方法 分析使用聚甲基(噁)嗪(PO)染色的全自動血細胞分析儀和用噻唑橙(TO)染色的傳統流式細胞分析儀檢測未成熟血小闆的批內精密度、攜帶汙染率等指標,分析同一儀器在不同時間檢測的未成熟血小闆百分比的差異,比較兩種方法的性能,檢測健康體檢人群(n =336)的未成熟血小闆百分比,分析兩種方法的相關性,統計全自動血細胞分析儀的參攷區間.結果 全自動血細胞分析儀的變異繫數(CV)為16.2％、攜帶汙染率為4.6％；流式細胞分析儀的最少CV為35.1％、攜帶汙染率為4.8％.樣本放置30 min、2h、4h後,全自動血細胞分析儀檢測未成熟血小闆,差異無統計學意義(P ＞0.05);TO染色後30 min、1h、2h用傳統流式細胞分析儀檢測的未成熟血小闆,差異有統計學意義(P＜0.01).兩種方法檢測結果有相關性(r2=0.923,P＜0.01).全自動血細胞分析儀檢測值的正常參攷區間為1.0％～7.5％；流式細胞分析儀檢測值的正常參攷區間為3.0％～10.5％.結論 全自動血細胞分析儀法比傳統流式細胞分析儀法操作簡單,結果穩定性好,是檢測未成熟血小闆的優選方法.
목적 분석비교검측미성숙혈소판적방법학차이,병조사본지구정상삼고구간,위실험실개전미성숙혈소판검측제공의거.방법 분석사용취갑기(오)진(PO)염색적전자동혈세포분석의화용새서등(TO)염색적전통류식세포분석의검측미성숙혈소판적비내정밀도、휴대오염솔등지표,분석동일의기재불동시간검측적미성숙혈소판백분비적차이,비교량충방법적성능,검측건강체검인군(n =336)적미성숙혈소판백분비,분석량충방법적상관성,통계전자동혈세포분석의적삼고구간.결과 전자동혈세포분석의적변이계수(CV)위16.2％、휴대오염솔위4.6％；류식세포분석의적최소CV위35.1％、휴대오염솔위4.8％.양본방치30 min、2h、4h후,전자동혈세포분석의검측미성숙혈소판,차이무통계학의의(P ＞0.05);TO염색후30 min、1h、2h용전통류식세포분석의검측적미성숙혈소판,차이유통계학의의(P＜0.01).량충방법검측결과유상관성(r2=0.923,P＜0.01).전자동혈세포분석의검측치적정상삼고구간위1.0％～7.5％；류식세포분석의검측치적정상삼고구간위3.0％～10.5％.결론 전자동혈세포분석의법비전통류식세포분석의법조작간단,결과은정성호,시검측미성숙혈소판적우선방법.
Objective To compare the accuracy,stability and sample cross-contamination of two independent methods of detecting the percentage of reticulated platelets in peripheral blood and establish a local normal reference range so as to provide methodological rationales in clinical laboratory.Methods The percentages of reticulated platelets in peripheral blood of a healthy population were measured by Sysmex XE-5000 blood cell analyzer with polymethyl oxazine staining and flow cytometer with thiazole orange staining respectively.The correlation between the results of two methods was analyzed by Spearman's nonparametric correlation.Information about stability was obtained from measurements of the percentages of reticulated platelets in peripheral blood at designated timepoints.The analyses of accuracy,sample cross contamination and local normal reference range were performed routinely.Results The coefficient of variation (CV) of data was lower (16.2％) than that from flow cytometer (35.1％).The sample cross-contaminations of two methods were the same at around 5％.The percentage of reticulated platelets in peripheral blood was stable and consistent whereas the results of flow cytometer fluctuated at different timepoints within 4 h after blood sampling.The correlation of results obtained from two methods was significant (P ＜ 0.01,r2 =0.923).The local normal reference range was 1.0％-7.5％ for Sysmex XE-5000 versus 3.0％-10.5％ for flow cytometer.Conclusions Fully automalic blood cell analyze is more advanced than flow cytometer for its simple operation and stable data.And the former is an ideal first-choice for detecting the percentage of reticulated platelets in peripheral blood.
