中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2012年
48期
3442-3444
,共3页
耳蜗%成像,三维%体层摄影术,X线计算机%无损成像
耳蝸%成像,三維%體層攝影術,X線計算機%無損成像
이와%성상,삼유%체층섭영술,X선계산궤%무손성상
Cochlea%Imaging,three-dimensional%Tomography,X-ray computed%Nondestructive imaging
目的 使用显微CT(micro-CT)设备研究耳蜗的无损三维成像,探讨该技术在内耳形态学方面的应用.方法 戊巴比妥钠麻醉豚鼠,处死后剥离耳蜗,戊二醛固定后使用micro-CT扫描,获得2D图像并3D建模重构.结果 获得了清晰的2D图像,耳蜗前庭阶、中阶、鼓阶形态完整,前庭膜、盖膜、Corti器、螺旋神经节等内耳微结构显像,3D图像立体透视良好,可做任意轴旋转.结论 使用micro-CT成像设备对豚鼠耳蜗进行无损成像,相较于传统的组织病理学方法,不但节省了标本,而且所获图像分辨率高,操作简便,比基于同步辐射光源的无损成像方法更易普及.
目的 使用顯微CT(micro-CT)設備研究耳蝸的無損三維成像,探討該技術在內耳形態學方麵的應用.方法 戊巴比妥鈉痳醉豚鼠,處死後剝離耳蝸,戊二醛固定後使用micro-CT掃描,穫得2D圖像併3D建模重構.結果 穫得瞭清晰的2D圖像,耳蝸前庭階、中階、鼓階形態完整,前庭膜、蓋膜、Corti器、螺鏇神經節等內耳微結構顯像,3D圖像立體透視良好,可做任意軸鏇轉.結論 使用micro-CT成像設備對豚鼠耳蝸進行無損成像,相較于傳統的組織病理學方法,不但節省瞭標本,而且所穫圖像分辨率高,操作簡便,比基于同步輻射光源的無損成像方法更易普及.
목적 사용현미CT(micro-CT)설비연구이와적무손삼유성상,탐토해기술재내이형태학방면적응용.방법 무파비타납마취돈서,처사후박리이와,무이철고정후사용micro-CT소묘,획득2D도상병3D건모중구.결과 획득료청석적2D도상,이와전정계、중계、고계형태완정,전정막、개막、Corti기、라선신경절등내이미결구현상,3D도상입체투시량호,가주임의축선전.결론 사용micro-CT성상설비대돈서이와진행무손성상,상교우전통적조직병이학방법,불단절성료표본,이차소획도상분변솔고,조작간편,비기우동보복사광원적무손성상방법경역보급.
Objective To employ micro-CT equipment for nondestructive three-dimensional (3D) imaging of internal ear.Methods The guinea pigs were anesthetized by napental and bilateral cochleas harvested.Cochlea was fixed in glutaraldehyde before scanning of micro-CT.Two-dimensional (2D) images were acquired for a 3D model of reconstruction.Results The 2D images was distinct enough to visualize vestibular gallery,scala media,scala tympani,Reissner's membrane,velum,organ of Corti and spiral ganglion,etc.The 3D structure model was excellent for viewing and free to revolve in any axial direction.Conclusions Micro-CT may allow nondestructive three-dimensional imaging of internal ear.As compared with the traditional method of morphology,this approach is able to save samples,easy to operate and has a high resolution.And it is more easily popularized than the synchrotron radiation approach.
