中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
2期
99-103
,共5页
欧阳涵%石永兵%苏楠%李凌云
歐暘涵%石永兵%囌楠%李凌雲
구양함%석영병%소남%리릉운
红斑狼疮,系统性%白细胞介素9%糖皮质激素
紅斑狼瘡,繫統性%白細胞介素9%糖皮質激素
홍반랑창,계통성%백세포개소9%당피질격소
Lupus erythematosus,systemic%Interleukin-9%Glucocorticoid
目的 研究系统性红斑狼疮(SLE)患者外周血中白细胞介素(IL)9 mRNA、IL-9蛋白水平以及CD4+ IL-9+T细胞百分比及其与临床指标的相关性,并观察糖皮质激素治疗对血浆IL-9水平及外周血CD4+ IL-9+T细胞百分比的影响.方法 采用实时荧光定量聚合酶链反应(real-time PCR)、酶联免疫吸附试验(ELISA)及流式细胞学分别检测28例SLE患者及12名健康体检者外周血中IL-9mRNA、IL-9蛋白水平及CD4+ IL-9+T细胞百分比,比较其在各组中的差异并分析糖皮质激素治疗对其的影响.结果 (1)SLE患者外周血IL-9mRNA的表达明显高于健康对照组(P＜0.01)；SLE活动组患者血浆IL-9水平及缓解组血浆IL-9水平均明显高于健康对照组[(68±11) ng/L、(56±14)ng/L比(26 ±6) ng/L,P＜0.01及P＜0.05]；SLE活动组患者外周血中CD4+ IL-9+T细胞百分比明显高于健康对照组和缓解组(1.96％±0.31％比0.28％±0.05％、0.89％±0.13％,均P＜0.01),缓解组亦高于健康对照组(P＜0.05)；(2)血浆IL-9水平和外周血CD4+ IL-9+T细胞百分比均与SLE活动性指数(SLEDAI)呈正相关；(3)初发活动期SLE患者在接受甲泼尼龙(0.8 mg· kg-1·d-1)治疗后第1、2、3周血浆IL-9水平及外周血 CD4+ IL-9+T细胞百分比均低于治疗前.结论 IL-9及CD4+ IL-9+T细胞可能在SLE的发病中起着重要作用,其含量异常可反映SLE的活动性及严重程度.
目的 研究繫統性紅斑狼瘡(SLE)患者外週血中白細胞介素(IL)9 mRNA、IL-9蛋白水平以及CD4+ IL-9+T細胞百分比及其與臨床指標的相關性,併觀察糖皮質激素治療對血漿IL-9水平及外週血CD4+ IL-9+T細胞百分比的影響.方法 採用實時熒光定量聚閤酶鏈反應(real-time PCR)、酶聯免疫吸附試驗(ELISA)及流式細胞學分彆檢測28例SLE患者及12名健康體檢者外週血中IL-9mRNA、IL-9蛋白水平及CD4+ IL-9+T細胞百分比,比較其在各組中的差異併分析糖皮質激素治療對其的影響.結果 (1)SLE患者外週血IL-9mRNA的錶達明顯高于健康對照組(P＜0.01)；SLE活動組患者血漿IL-9水平及緩解組血漿IL-9水平均明顯高于健康對照組[(68±11) ng/L、(56±14)ng/L比(26 ±6) ng/L,P＜0.01及P＜0.05]；SLE活動組患者外週血中CD4+ IL-9+T細胞百分比明顯高于健康對照組和緩解組(1.96％±0.31％比0.28％±0.05％、0.89％±0.13％,均P＜0.01),緩解組亦高于健康對照組(P＜0.05)；(2)血漿IL-9水平和外週血CD4+ IL-9+T細胞百分比均與SLE活動性指數(SLEDAI)呈正相關；(3)初髮活動期SLE患者在接受甲潑尼龍(0.8 mg· kg-1·d-1)治療後第1、2、3週血漿IL-9水平及外週血 CD4+ IL-9+T細胞百分比均低于治療前.結論 IL-9及CD4+ IL-9+T細胞可能在SLE的髮病中起著重要作用,其含量異常可反映SLE的活動性及嚴重程度.
목적 연구계통성홍반랑창(SLE)환자외주혈중백세포개소(IL)9 mRNA、IL-9단백수평이급CD4+ IL-9+T세포백분비급기여림상지표적상관성,병관찰당피질격소치료대혈장IL-9수평급외주혈CD4+ IL-9+T세포백분비적영향.방법 채용실시형광정량취합매련반응(real-time PCR)、매련면역흡부시험(ELISA)급류식세포학분별검측28례SLE환자급12명건강체검자외주혈중IL-9mRNA、IL-9단백수평급CD4+ IL-9+T세포백분비,비교기재각조중적차이병분석당피질격소치료대기적영향.결과 (1)SLE환자외주혈IL-9mRNA적표체명현고우건강대조조(P＜0.01)；SLE활동조환자혈장IL-9수평급완해조혈장IL-9수평균명현고우건강대조조[(68±11) ng/L、(56±14)ng/L비(26 ±6) ng/L,P＜0.01급P＜0.05]；SLE활동조환자외주혈중CD4+ IL-9+T세포백분비명현고우건강대조조화완해조(1.96％±0.31％비0.28％±0.05％、0.89％±0.13％,균P＜0.01),완해조역고우건강대조조(P＜0.05)；(2)혈장IL-9수평화외주혈CD4+ IL-9+T세포백분비균여SLE활동성지수(SLEDAI)정정상관；(3)초발활동기SLE환자재접수갑발니룡(0.8 mg· kg-1·d-1)치료후제1、2、3주혈장IL-9수평급외주혈 CD4+ IL-9+T세포백분비균저우치료전.결론 IL-9급CD4+ IL-9+T세포가능재SLE적발병중기착중요작용,기함량이상가반영SLE적활동성급엄중정도.
Objective To examine the protein and mRNA levels of interleukin-9 (IL-9)and the frequencies of CD4 + IL-9 + T-cells in peripheral blood of patients with systemic lupus erytbematosus (SLE)and explore the roles of double positive T cells and IL-9 in the pathogenesis of SLE and the effects of glucocorticoid.Methods Twenty-eight hospitalized SLE patients were recruited and 12 healthy volunteers selected as normal controls.The mRNA levels of IL-9 in peripheral blood were measured by real timepolymerase chain reaction (RT-PCR),plasma protein of IL-9 by enzyme-linked immunosorbent assay (ELISA) and frequencies of CD4+ IL-9 + T-cells by flow cytometry.And the differences between two groups and the effects of glucocorticoid were analyzed.Results (1) The mRNA levels of IL-9 were significantly elevated in SLE patients as compared with normal controls (P ＜ 0.01).The serum levels of IL-9 were significantly higher in active and inactive SLE patients than those in healthy individuals (68 ± 11 vs 26 ± 6 ng/L,P ＜ 0.01 ; 56 ± 14 vs 26 ± 6 ng/L,P ＜ 0.05).The percentages of CD4 + IL-9 + T-cells increased in active SLE patients (1.96％ ± 0.31％) versus inactive SLE patients (0.89％ ± 0.13％,P ＜ 0.01) and healthy controls (0.28％ ±0.05％,P ＜ 0.001).And it was higher in inactive SLE patients than that in controls (P ＜ 0.05).(2) The serum levels of IL-9 and the frequencies of CD4 + IL-9 + T-cells were positively correlated with SLE disease activity index (SLEDAI).(3) The frequencies of CD4 + IL-9 + T-cells and the serum levels of IL-9 in 8 untreated active SLE patients decreased at weeks 1,2 and 3 after the therapy of methylprednisolone (0.8 mg · kg-1 · d-1) versus those at pre-treatment.Conclusion The abnormalities of IL-9 and CD4 + IL-9 + T-cells may play an important role in the pathogenesis of SLE.And the frequencies of CD4 + IL-9 + T-cells and the levels of IL-9 are evaluative parameters of SLE activity and severity.
