中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
5期
385-388
,共4页
姜黄素%CIK%卵巢癌细胞%细胞凋亡
薑黃素%CIK%卵巢癌細胞%細胞凋亡
강황소%CIK%란소암세포%세포조망
Curcumin%Cytokine-induced killer cells%Ovarian cancer cells%Apoptosis
目的 观察姜黄素(Cur)联合人类细胞因子诱导的杀伤细胞(CIK)对卵巢癌SKOV3细胞诱导细胞凋亡的作用,并探讨其可能的分子机制.方法 诱导脐血CIK细胞,电镜下观察Cur、CIK细胞、Cur联合CIK细胞作用后SKOV3细胞凋亡形态,RT-PCR检测各组细胞Fas、FADD、Caspase3mRNA的表达.将不同浓度的Cur分别作用于SKOV3细胞及CIK细胞,免疫印迹检测SKOV3细胞表面Fas表达及CIK细胞表面FasL的表达.MTT法检测抗FasL单克隆抗体对CIK细胞及Cur联合CIK细胞对SKOV3细胞增殖抑制率的影响.结果 Cur联合CIK组与Cur组和CIK组相比,细胞凋亡的形态学改变最明显.Cur联合CIK组与Cur组和CIK组相比,SKOV3细胞Fas及Caspase3的mRNA表达水平增加(P＜0.05)；Cur组、CIK组和Cur联合CIK组对SKOV3细胞FADD的mRNA的表达无明显变化.Cur能够促进SKOV3细胞表面Fas蛋白表达,促进CIK细胞膜FasL蛋白表达.CIK组和Cur联合CIK组FasL抗体预先孵育能够明显抑制对SKOV3细胞的增殖抑制作用.结论 Cur与CIK细胞联合使用诱导SKOV3细胞凋亡作用增强,其机制可能为Cur促进SKOV3细胞表面Fas表达增强,CIK细胞表面FasL表达增强,从而使SKOV3细胞内Fas表达增加,最终使Caspase3表达增高.
目的 觀察薑黃素(Cur)聯閤人類細胞因子誘導的殺傷細胞(CIK)對卵巢癌SKOV3細胞誘導細胞凋亡的作用,併探討其可能的分子機製.方法 誘導臍血CIK細胞,電鏡下觀察Cur、CIK細胞、Cur聯閤CIK細胞作用後SKOV3細胞凋亡形態,RT-PCR檢測各組細胞Fas、FADD、Caspase3mRNA的錶達.將不同濃度的Cur分彆作用于SKOV3細胞及CIK細胞,免疫印跡檢測SKOV3細胞錶麵Fas錶達及CIK細胞錶麵FasL的錶達.MTT法檢測抗FasL單剋隆抗體對CIK細胞及Cur聯閤CIK細胞對SKOV3細胞增殖抑製率的影響.結果 Cur聯閤CIK組與Cur組和CIK組相比,細胞凋亡的形態學改變最明顯.Cur聯閤CIK組與Cur組和CIK組相比,SKOV3細胞Fas及Caspase3的mRNA錶達水平增加(P＜0.05)；Cur組、CIK組和Cur聯閤CIK組對SKOV3細胞FADD的mRNA的錶達無明顯變化.Cur能夠促進SKOV3細胞錶麵Fas蛋白錶達,促進CIK細胞膜FasL蛋白錶達.CIK組和Cur聯閤CIK組FasL抗體預先孵育能夠明顯抑製對SKOV3細胞的增殖抑製作用.結論 Cur與CIK細胞聯閤使用誘導SKOV3細胞凋亡作用增彊,其機製可能為Cur促進SKOV3細胞錶麵Fas錶達增彊,CIK細胞錶麵FasL錶達增彊,從而使SKOV3細胞內Fas錶達增加,最終使Caspase3錶達增高.
목적 관찰강황소(Cur)연합인류세포인자유도적살상세포(CIK)대란소암SKOV3세포유도세포조망적작용,병탐토기가능적분자궤제.방법 유도제혈CIK세포,전경하관찰Cur、CIK세포、Cur연합CIK세포작용후SKOV3세포조망형태,RT-PCR검측각조세포Fas、FADD、Caspase3mRNA적표체.장불동농도적Cur분별작용우SKOV3세포급CIK세포,면역인적검측SKOV3세포표면Fas표체급CIK세포표면FasL적표체.MTT법검측항FasL단극륭항체대CIK세포급Cur연합CIK세포대SKOV3세포증식억제솔적영향.결과 Cur연합CIK조여Cur조화CIK조상비,세포조망적형태학개변최명현.Cur연합CIK조여Cur조화CIK조상비,SKOV3세포Fas급Caspase3적mRNA표체수평증가(P＜0.05)；Cur조、CIK조화Cur연합CIK조대SKOV3세포FADD적mRNA적표체무명현변화.Cur능구촉진SKOV3세포표면Fas단백표체,촉진CIK세포막FasL단백표체.CIK조화Cur연합CIK조FasL항체예선부육능구명현억제대SKOV3세포적증식억제작용.결론 Cur여CIK세포연합사용유도SKOV3세포조망작용증강,기궤제가능위Cur촉진SKOV3세포표면Fas표체증강,CIK세포표면FasL표체증강,종이사SKOV3세포내Fas표체증가,최종사Caspase3표체증고.
Objective To observe the pro-apoptotic effects of Curcumin associated with CIK cells against SKOV3 cells of ovarian carcinoma and discusses the possible molecular mechanisms.Methods CIK cells were induced from umbilicus cord blood.The apoptotic morphology of SKOV3 cells was observed under electron microscope after treated with Cur,CIK cells and Cur associated with CIK cells.The levels of Fas protein on surface of ovarian cancer cells and FasL protein on surface of CIK cells after Curcumin treatment were determined by Western blot.The inhibition rates on proliferation of CIK cells and Cur associated with CIK cells after addition of FasL monoclonal antibody were detected by (thiazolyl blue tetrazolium bromide) MTT.Results The changes of apoptotic morphology in the group of Cur associated with CIK cells were most obvious compared with that in the group of Cur or CIK cells alone.Cur could promote the expression of Fas on surface of SKOV3 cells and FasL on membranes of CIK cells.The inhibition rates on proliferation in the group of CIK ceils and Cur associated with CIK cells could be restrained obviously after an addition of antiFasmAb.Conclusion The pro-apoptotic effects of SKOV3 cells increase with the combined use of Cur and CIK cells.The mechanism may be that Cur can promote the expression of Fas protein on cell surface of SKOV3 cells and FasL protein on cell membrane of CIK cells so as to up-regulate the expression of Fas protein in SKOV3 cells and lead ultimately to the a higher expression of Caspase3.
