中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
7期
550-553
,共4页
申法政%王峰%杨光明%苗林%王恩杰%徐进%徐永龙%孙涛
申法政%王峰%楊光明%苗林%王恩傑%徐進%徐永龍%孫濤
신법정%왕봉%양광명%묘림%왕은걸%서진%서영룡%손도
癫痫%电刺激%海马%GABAA受体%亚基
癲癇%電刺激%海馬%GABAA受體%亞基
전간%전자격%해마%GABAA수체%아기
Epilepsy%Electrical stimulation%Hipocampus%GABAA receptors%Subunit
目的 探讨低频电刺激(LFS)海马对海人酸致癫痫大鼠突触外GABAA受体α5亚基的影响及LFS海马可能的治疗机制.方法 雄性SD大鼠成功致癫痫模型24只按随机数字表法分为癫痫组、假手术组和LFS海马治疗组,以及正常对照组大鼠8只.通过行为学、实时荧光定量聚合酶链式反应和Westen印迹,分析突触外GABAA受体α5亚基表达变化情况及其与LFS海马治疗癫痫疗效之间的关系.结果 在治疗开始后癫痫组、假手术组、LFS海马治疗组癫痫发作次数分别为(8.50 ±0.93)次、(8.88 ±0.83)次、(2.88 ±0.83)次,LFS海马治疗组癫痫发作次数明显减少(P＜0.01).实时荧光定量PCR检测显示癫痫组、假手术组、LFS海马治疗组、正常对照组α5亚基mRNA表达分别为0.30±0.16、0.31±0.16、0.74±0.20、1.10±0.23；Westen印迹显示癫痫组、假手术组、LFS海马治疗组、正常对照组α5亚基蛋白表达分别为0.22±0.08、0.26±0.08、0.75±0.09、0.88±0.09.LFS海马治疗组与癫痫组、假手术组之间α5亚基mRNA及相关蛋白比较差异有统计学意义(P＜0.01),仍低于正常对照组表达水平(P＜0.01).结论 LFS海马可以有效抑制癫痫发作,可能与α5亚基的表达水平密切相关.
目的 探討低頻電刺激(LFS)海馬對海人痠緻癲癇大鼠突觸外GABAA受體α5亞基的影響及LFS海馬可能的治療機製.方法 雄性SD大鼠成功緻癲癇模型24隻按隨機數字錶法分為癲癇組、假手術組和LFS海馬治療組,以及正常對照組大鼠8隻.通過行為學、實時熒光定量聚閤酶鏈式反應和Westen印跡,分析突觸外GABAA受體α5亞基錶達變化情況及其與LFS海馬治療癲癇療效之間的關繫.結果 在治療開始後癲癇組、假手術組、LFS海馬治療組癲癇髮作次數分彆為(8.50 ±0.93)次、(8.88 ±0.83)次、(2.88 ±0.83)次,LFS海馬治療組癲癇髮作次數明顯減少(P＜0.01).實時熒光定量PCR檢測顯示癲癇組、假手術組、LFS海馬治療組、正常對照組α5亞基mRNA錶達分彆為0.30±0.16、0.31±0.16、0.74±0.20、1.10±0.23；Westen印跡顯示癲癇組、假手術組、LFS海馬治療組、正常對照組α5亞基蛋白錶達分彆為0.22±0.08、0.26±0.08、0.75±0.09、0.88±0.09.LFS海馬治療組與癲癇組、假手術組之間α5亞基mRNA及相關蛋白比較差異有統計學意義(P＜0.01),仍低于正常對照組錶達水平(P＜0.01).結論 LFS海馬可以有效抑製癲癇髮作,可能與α5亞基的錶達水平密切相關.
목적 탐토저빈전자격(LFS)해마대해인산치전간대서돌촉외GABAA수체α5아기적영향급LFS해마가능적치료궤제.방법 웅성SD대서성공치전간모형24지안수궤수자표법분위전간조、가수술조화LFS해마치료조,이급정상대조조대서8지.통과행위학、실시형광정량취합매련식반응화Westen인적,분석돌촉외GABAA수체α5아기표체변화정황급기여LFS해마치료전간료효지간적관계.결과 재치료개시후전간조、가수술조、LFS해마치료조전간발작차수분별위(8.50 ±0.93)차、(8.88 ±0.83)차、(2.88 ±0.83)차,LFS해마치료조전간발작차수명현감소(P＜0.01).실시형광정량PCR검측현시전간조、가수술조、LFS해마치료조、정상대조조α5아기mRNA표체분별위0.30±0.16、0.31±0.16、0.74±0.20、1.10±0.23；Westen인적현시전간조、가수술조、LFS해마치료조、정상대조조α5아기단백표체분별위0.22±0.08、0.26±0.08、0.75±0.09、0.88±0.09.LFS해마치료조여전간조、가수술조지간α5아기mRNA급상관단백비교차이유통계학의의(P＜0.01),잉저우정상대조조표체수평(P＜0.01).결론 LFS해마가이유효억제전간발작,가능여α5아기적표체수평밀절상관.
Objective To explore the effects of low-frequency electric stimulus (LFS) on hippocampal of or5 subunit of extrasynaptic GABAA receptor in kainic acid-induced epilepsy rats and explore the possible mechanism of LFS on hippocampus to treat epilepsy.Methods A total of 32 male SpragueDawley rats were divided randomly into 4 groups:epileptic,pseudo-surgery,LFS treatment and control.The expression of α5 subunit of extrasynaptic GABAA receptor and the association with the curative effects of LFS were evaluated by behavioristics,real-time fluorogenic quantitative polymerase chain reaction (PCR) and Western blot.Result Ater treatment,the number of seizures in LFS group were significantly lower than that in the epileptic and pseudo-surgery groups (2.88 ± 0.83 vs 8.50 ± 0.93 and 8.88 ± 0.83)(P ＜ 0.01).The results of real-time PCR demonstrated that the expression of mRNA of α5 subunits in LFS treatment group was obviously higher than that in epileptic and pseudo-surgery groups (0.74 ± 0.20 vs 0.30 ±0.16 and 0.31 ±0.16) (P＜0.01),but was lower than 1.10 ±0.23 in control group.Western blot demonstrated that the protein expression of α5 subunits in LFS (0.75 ± 0.09) and control (0.88 ± 0.09)groups were dramatically higher than that in epileptic (0.22 ± 0.08) and pseudo-surgery (0.26 ± 0.08)groups (P ＜ 0.01).Conclusion Low-frequency electric stimulus on hippocampus is effective in controlling epileptic seizures.And the mechanism may be related with the expression level of α5 subunits of extrasynaptic GABAA receptor.
