CAJ | 학술논문

目的探索影像辅助式胸腔镜微创手术(VATS)建立糖尿病小型猪急性心肌梗死(AMI)模型的新方法.方法健康和糖尿病小型猪各7只,在电视放大图像指导下行胸腔镜微创手术,定点结扎冠状动脉左前降支,然后通过血清学、影像学和组织学手段评价模型.结果两组动物术后血清肌钙蛋白和肌红蛋白均增至参考值上限的10倍以上.超声心动图、磁共振成像(MRI)和组织病理分析表明,VATS后两组心肌受累部位均为心尖、左室前壁和室间隔中远段.但糖尿病小型猪的心脏功能相对低下；梗死区与梗死危险区比值更高(18.4％±5.5％比5.3％±3.9％,P=0.03)；心肌梗死透壁度更重(75％以上),符合糖尿病心肌缺血耐受性差的疾病特征.结论 VATS是一种制备糖尿病小型猪AMI模型的安全、有效方法.
목적 탐색영상보조식흉강경미창수술(VATS)건립당뇨병소형저급성심기경사(AMI)모형적신방법.방법 건강화당뇨병소형저각7지,재전시방대도상지도하행흉강경미창수술,정점결찰관상동맥좌전강지,연후통과혈청학、영상학화조직학수단평개모형.결과 량조동물술후혈청기개단백화기홍단백균증지삼고치상한적10배이상.초성심동도、자공진성상(MRI)화조직병리분석표명,VATS후량조심기수루부위균위심첨、좌실전벽화실간격중원단.단당뇨병소형저적심장공능상대저하；경사구여경사위험구비치경고(18.4％±5.5％비5.3％±3.9％,P=0.03)；심기경사투벽도경중(75％이상),부합당뇨병심기결혈내수성차적질병특정.결론 VATS시일충제비당뇨병소형저AMI모형적안전、유효방법.
Objective To explore a new method of establishing acute myocardial infarction model in diabetic miniature pigs through video-assisted thoracoscopic surgery (VATS).Methods Seven normal miniature pigs and 7 diabetic miniature pigs underwent VATS by selectively ligating left anterior descending coronary artery and then were evaluated through serology,imaging and histology.Results The serum levels of troponin (cTni) and myoglohin (MYO) in both groups significantly increased by over 10 folds of upper normal limit after VATS.Echocardiography,MRI and histopathologic analysis showed that the affected myocardial parts were apex,left ventricular wall and interventricular septum cosco section.But heart function of diabetic miniature pigs were relatively lower; infarction area/area-at-risk ratio higher(18.4％ ± 5.5％ vs 5.3％ ±3.9％,P =0.03),myocardial infarction through-wall degree more severe.These were in accordance with poor ischemic tolerance in diabetic myocardia.Conclusion VATS is a safe and effective method for establishing AMI model in diabetic miniature pigs.