中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
25期
1989-1992
,共4页
秦姗%阎雪%唐红娟%周英杰%刘影%沈鸿敏%李晓冬
秦姍%閻雪%唐紅娟%週英傑%劉影%瀋鴻敏%李曉鼕
진산%염설%당홍연%주영걸%류영%침홍민%리효동
多囊卵巢综合征%子宫内膜%血管紧张素转换酶2%血管紧张-(1-7)
多囊卵巢綜閤徵%子宮內膜%血管緊張素轉換酶2%血管緊張-(1-7)
다낭란소종합정%자궁내막%혈관긴장소전환매2%혈관긴장-(1-7)
Polycystic ovary syndrome%Endometrium%Angiotensin-converting enzyme type 2%Angiotensin-(1-7)
目的 检测正常月经周期子宫内膜和多囊卵巢综合征(PCOS)患者子宫内膜上肾素-血管紧张素系统(RAS)各组分的表达.方法 选取正常月经周期子宫内膜,其中增殖期和分泌期各30例,采用免疫组化SP法检测AngⅡ、AT1R、AT2R、ACE2、Ang-(1-7)、Mas在子宫内膜蛋白水平的表达.收集PCOS患者增殖期子宫内膜15例,正常增殖期子宫内膜15例.常规HE染色判定子宫内膜标本的组织学分期；实时荧光相对定量聚合酶链反应(RQ-PCR)检测AT1R、AT2R、ACE2及Mas在子宫内膜分子水平的表达.结果 正常月经周期子宫内膜AngⅡAT1R、AT2R、ACE2、Ang-(1-7)及Mas的表达呈周期性改变,趋势相似,腺上皮的表达强于间质,分泌期强于增殖期.PCOS患者增殖期子宫内膜中ACE2 mRNA、AT1R mRNA、AT2R mRNA、Mas mRNA明显高于对照组(P＜0.01).结论 宫内膜局部存在RAS系统,其各组分存在着周期性变化.PCOS患者子宫内膜中AT1R、AT2R、ACE2、Mas分子水平表达增强.
目的 檢測正常月經週期子宮內膜和多囊卵巢綜閤徵(PCOS)患者子宮內膜上腎素-血管緊張素繫統(RAS)各組分的錶達.方法 選取正常月經週期子宮內膜,其中增殖期和分泌期各30例,採用免疫組化SP法檢測AngⅡ、AT1R、AT2R、ACE2、Ang-(1-7)、Mas在子宮內膜蛋白水平的錶達.收集PCOS患者增殖期子宮內膜15例,正常增殖期子宮內膜15例.常規HE染色判定子宮內膜標本的組織學分期；實時熒光相對定量聚閤酶鏈反應(RQ-PCR)檢測AT1R、AT2R、ACE2及Mas在子宮內膜分子水平的錶達.結果 正常月經週期子宮內膜AngⅡAT1R、AT2R、ACE2、Ang-(1-7)及Mas的錶達呈週期性改變,趨勢相似,腺上皮的錶達彊于間質,分泌期彊于增殖期.PCOS患者增殖期子宮內膜中ACE2 mRNA、AT1R mRNA、AT2R mRNA、Mas mRNA明顯高于對照組(P＜0.01).結論 宮內膜跼部存在RAS繫統,其各組分存在著週期性變化.PCOS患者子宮內膜中AT1R、AT2R、ACE2、Mas分子水平錶達增彊.
목적 검측정상월경주기자궁내막화다낭란소종합정(PCOS)환자자궁내막상신소-혈관긴장소계통(RAS)각조분적표체.방법 선취정상월경주기자궁내막,기중증식기화분비기각30례,채용면역조화SP법검측AngⅡ、AT1R、AT2R、ACE2、Ang-(1-7)、Mas재자궁내막단백수평적표체.수집PCOS환자증식기자궁내막15례,정상증식기자궁내막15례.상규HE염색판정자궁내막표본적조직학분기；실시형광상대정량취합매련반응(RQ-PCR)검측AT1R、AT2R、ACE2급Mas재자궁내막분자수평적표체.결과 정상월경주기자궁내막AngⅡAT1R、AT2R、ACE2、Ang-(1-7)급Mas적표체정주기성개변,추세상사,선상피적표체강우간질,분비기강우증식기.PCOS환자증식기자궁내막중ACE2 mRNA、AT1R mRNA、AT2R mRNA、Mas mRNA명현고우대조조(P＜0.01).결론 궁내막국부존재RAS계통,기각조분존재착주기성변화.PCOS환자자궁내막중AT1R、AT2R、ACE2、Mas분자수평표체증강.
Objective To explore the expression of renin-angiotensin system component (ACE-Ang Ⅱ-AT1/AT2 and ACE2-Ang-(1-7)-Mas) in endometrium of polycystic ovary syndrome (PCOS) patients and normal controls.Methods Thirty cases of normal endometrium in proliferative and secretory phases respectively were selected for the protein levels of Ang Ⅱ,AT1,AT2,ACE2,Ang-(1-7) and Mas through immunohistochemistry.And the specimens of proliferative endometrium from 15 PCOS patients and 15 normal controls respectively were prepared for the expressions of AT1,AT2,ACE2 and Mas mRNA through relative quantitative-polymerase chain reaction (RQ-PCR).The histological phases of endometria were confirmed by hematoxylin and eosin staining under microscope.Results The expressions of Ang Ⅱ,AT1R,AT2R,ACE2,Ang-(1-7) and Mas showed periodical changes in endometrium throughout normal menstrual cycles and shared a similar trend.The expression was more pronounced in epithelial cells than that in stromal cells while it was also more dramatic in secretory phase than proliferative phase; the mRNA expressions of AT1,AT2,ACE2 and MAS were higher in PCOS endometrium than those in normal controls.Statistically significant differences existed between two groups (P ＜ 0.01).Conclusion There is local existence of RAS in endometrium; increased expressions of AT1 mRNA,AT2 mRNA,ACE2 mRNA and Mas mRNA in endometrium of PCOS may influence endometrial development and play a role in the pathological process of PCOS.
