中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2013年
25期
2001-2003
,共3页
刘彬%刘志峰%任炳成%陈聪%于圣平%张斌%明浩朗%王垒垒%赵恺
劉彬%劉誌峰%任炳成%陳聰%于聖平%張斌%明浩朗%王壘壘%趙愷
류빈%류지봉%임병성%진총%우골평%장빈%명호랑%왕루루%조개
神经胶质瘤%细胞运动%高尔基体
神經膠質瘤%細胞運動%高爾基體
신경효질류%세포운동%고이기체
Glioma%Cell migration%Golgi apparatus
目的 观察高尔基体与细胞运动方向的关系,探讨高尔基体作为细胞运动方向指示的可行性及其在研究细胞迁移运动方面的生物学意义.方法 选择大鼠C6胶质瘤细胞、人脑U251和SNB19胶质瘤细胞,通过细胞免疫荧光染色观察细胞迁移实验中高尔基体与细胞运动方向的关系,计数高尔基体朝向划痕空白区的细胞所占的百分比；鬼笔环肽细胞骨架染色,观察高尔基体与细胞运动伪足的位置关系；免疫组化染色观大鼠脑胶质瘤组织标本和人脑胶质瘤组织标本中坏死区周围细胞高尔基体的定位与细胞运动方向的关系,计数高尔基体背离坏死区的细胞所占百分比.结果 细胞迁移实验中,位于划痕两侧的绝大多数C6细胞(83％±6％)、U251细胞(80％±7％)、SNB19细胞(82％±6％)的高尔基体朝向划痕空白侧；鬼笔环肽细胞骨架染色显示U251细胞和SNB19细胞中高尔基体的定位方向与细胞的伪足伸展方向一致.免疫组化染色显示,肿瘤组织标本中坏死区周围肿瘤细胞的高尔基体多数(鼠脑80％±7％；人脑82％±6％)背向坏死侧.结论 无论在体内还是在体外环境中,细胞高尔基体的定位方向对于判断细胞的迁移运动方向都具有参考意义,从而为在体研究肿瘤细胞侵袭的生物学行为和抗迁移治疗疗效的观察提供了有力的观测指标.
目的 觀察高爾基體與細胞運動方嚮的關繫,探討高爾基體作為細胞運動方嚮指示的可行性及其在研究細胞遷移運動方麵的生物學意義.方法 選擇大鼠C6膠質瘤細胞、人腦U251和SNB19膠質瘤細胞,通過細胞免疫熒光染色觀察細胞遷移實驗中高爾基體與細胞運動方嚮的關繫,計數高爾基體朝嚮劃痕空白區的細胞所佔的百分比；鬼筆環肽細胞骨架染色,觀察高爾基體與細胞運動偽足的位置關繫；免疫組化染色觀大鼠腦膠質瘤組織標本和人腦膠質瘤組織標本中壞死區週圍細胞高爾基體的定位與細胞運動方嚮的關繫,計數高爾基體揹離壞死區的細胞所佔百分比.結果 細胞遷移實驗中,位于劃痕兩側的絕大多數C6細胞(83％±6％)、U251細胞(80％±7％)、SNB19細胞(82％±6％)的高爾基體朝嚮劃痕空白側；鬼筆環肽細胞骨架染色顯示U251細胞和SNB19細胞中高爾基體的定位方嚮與細胞的偽足伸展方嚮一緻.免疫組化染色顯示,腫瘤組織標本中壞死區週圍腫瘤細胞的高爾基體多數(鼠腦80％±7％；人腦82％±6％)揹嚮壞死側.結論 無論在體內還是在體外環境中,細胞高爾基體的定位方嚮對于判斷細胞的遷移運動方嚮都具有參攷意義,從而為在體研究腫瘤細胞侵襲的生物學行為和抗遷移治療療效的觀察提供瞭有力的觀測指標.
목적 관찰고이기체여세포운동방향적관계,탐토고이기체작위세포운동방향지시적가행성급기재연구세포천이운동방면적생물학의의.방법 선택대서C6효질류세포、인뇌U251화SNB19효질류세포,통과세포면역형광염색관찰세포천이실험중고이기체여세포운동방향적관계,계수고이기체조향화흔공백구적세포소점적백분비；귀필배태세포골가염색,관찰고이기체여세포운동위족적위치관계；면역조화염색관대서뇌효질류조직표본화인뇌효질류조직표본중배사구주위세포고이기체적정위여세포운동방향적관계,계수고이기체배리배사구적세포소점백분비.결과 세포천이실험중,위우화흔량측적절대다수C6세포(83％±6％)、U251세포(80％±7％)、SNB19세포(82％±6％)적고이기체조향화흔공백측；귀필배태세포골가염색현시U251세포화SNB19세포중고이기체적정위방향여세포적위족신전방향일치.면역조화염색현시,종류조직표본중배사구주위종류세포적고이기체다수(서뇌80％±7％；인뇌82％±6％)배향배사측.결론 무론재체내환시재체외배경중,세포고이기체적정위방향대우판단세포적천이운동방향도구유삼고의의,종이위재체연구종류세포침습적생물학행위화항천이치료료효적관찰제공료유력적관측지표.
Objective To explore the relationship between Golgi apparatus and the direction of tumor cell migration in vivo and in vitro.Methods Cell migration assays were conducted with rat C6 glioma cells,human U251 and SNB19 glioma cells respectively.Then immunofluorescence was used to detect the position of Golgi apparatus in migrating cells.The percentage of cells with Golgi apparatus facing towards wound edge was calculated.Cell pseudopodium was stained with TRITC-phalloidin and the relationship between Golgi apparatus and pseudopodium detected.Immunohistochemistry was used to reveal the Golgi apparatus in tumor tissue samples.And the percentage of cells with Golgi apparatus facing opposite to the necrotic zones was calculated.Results In cells located at wound edge,the Golgi apparatus was found facing towards the wound in the vast majority of cells (C6 83％ ±6％,U251 80％ ±7％,SNB19 82％ ± 6％).In U251 and SNB19 cells,the golgi apparatus was located in the same direction with cellular pseudopodium.Immunohistochemical staining showed that in cells located around the necrotic zone,the Golgi apparatus faced opposite to the necrotic zones in most cells (rat tissue samples 80％ ± 7％,human tissue samples 82％ ± 6％).Conclusion The Golgi apparatus is closely correlated with cell migration and it may be considered as a direction indicator of cell migration.And it provides an important index for the study of tumor cell invasion both in vivo and in vitro.
