CAJ | 학술논문

目的分析北京市HIV-1 B’/C重组型毒株的分子流行特征.方法采集北京市2006-2010年新发现B'/C重组型HIV-1感染者的抗凝全血标本,分离血浆和提取病毒RNA,用反转录/巢式聚合酶链式反应扩增病毒gag基因,并进行系统进化分析.结果成功扩增了159例标本,CRF07_BC 147例,CRF08_BC 12例,CRF07_BC系统进化树中存在3个主要的亚簇,IDU-Max亚簇包含89例样本,静脉吸毒传播占71.9％,同参考株97CN001组间离散率为2.8％;IDU-Min亚簇包含22例样本,静脉吸毒传播占77.3％,组间离散率为2.8％;MSM亚簇包含34例样本,男男同性传播占67.6％,组间离散率为3.1％;3个亚簇内均无其他国家的序列,只有IDU-Max亚簇包含1条来自中国台湾的序列;核苷酸多态性分析结果显示,以IDU-Max亚簇为参照,IDU-Min和MSM亚簇分别有32和41个位点的核苷酸组成存在著性差异.结论首次观察到北京市流行的CRF07_BC病毒株中存在3个独立的亚簇,异性传播和静脉吸毒传播序列混杂分布,而男同传播病例则聚集成1个亚簇,应当继续对B’/C重组型分子流行特征进行监测.
목적 분석북경시HIV-1 B’/C중조형독주적분자류행특정.방법 채집북경시2006-2010년신발현B'/C중조형HIV-1감염자적항응전혈표본,분리혈장화제취병독RNA,용반전록/소식취합매련식반응확증병독gag기인,병진행계통진화분석.결과 성공확증료159례표본,CRF07_BC 147례,CRF08_BC 12례,CRF07_BC계통진화수중존재3개주요적아족,IDU-Max아족포함89례양본,정맥흡독전파점71.9％,동삼고주97CN001조간리산솔위2.8％;IDU-Min아족포함22례양본,정맥흡독전파점77.3％,조간리산솔위2.8％;MSM아족포함34례양본,남남동성전파점67.6％,조간리산솔위3.1％;3개아족내균무기타국가적서렬,지유IDU-Max아족포함1조래자중국태만적서렬;핵감산다태성분석결과현시,이IDU-Max아족위삼조,IDU-Min화MSM아족분별유32화41개위점적핵감산조성존재저성차이.결론 수차관찰도북경시류행적CRF07_BC병독주중존재3개독립적아족,이성전파화정맥흡독전파서렬혼잡분포,이남동전파병례칙취집성1개아족,응당계속대B’/C중조형분자류행특정진행감측.
Objective To analyze the molecular epidemiological characteristic of HIV-1 B'/C strains prevalent in Beijing.Methods Plasma samples were collected from 200 newly diagnosed HIV-1 B'/C individuals reported during 2006 to 2010 in Beijing.The gag gene fragments were amplified from RNA template extracted from plasma with reverse transcription (RT) and nested polymerase chain reaction (PCR).And the sequences were analyzed by phylogenetic methods and Entropy analysis.Results A total of 159 sequences were successfully amplified from the gag genes of which 147 was CRF07_BC and 12 CRF08_BC.There were 3 main subclusters in CRF07_BC phylogenetic tree and they were named as subcluster IDUMax (89 sequences),subcluster IDU-Min (22 sequences) and subcluster MSM (34 sequences) based on transmission.No international reference strain was closely related with these three subclusters except for one strain identified in Taiwan.All CRF07_BC recombinant strains were remarkable for their low interpatient diversity in gag genes (3.7％,3.3％ and 2.0％ for isolates from IDU-Max,IDU-Min and MSM respectively).When compared with subcluster IDU-Max,there were 32 and 41 significantly different sites of nucleotide polymorphism compositions in subclusers IDU-Min and MSM.Conclusion This is the first report of describing the existence of three main epidemic subclusters in CRF07_BC strains prevalent in Beijing.And IDU-Max subcluster is the dominant strain.The CRF07_BC in Beijing are less diverse than other strains and may be derived from a common ancestor.